Vaginal Microbial Environment Skews Macrophage Polarization and Contributes to Cervical Cancer Development
As a common female reproductive system malignancy, cervical cancer (CC) disturbs numerous women’s health. This study demonstrates the role of the vaginal microbial environment (Peptostreptococcus anaerobius) in cervical cancer. Functional assays, including cell proliferation assay, tube formation as...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/3525735 |
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| author | Guannan Zhou Fangyue Zhou Yuanyuan Gu Menglei Zhang Ganrong Zhang Fang Shen Keqin Hua Jingxin Ding |
| author_facet | Guannan Zhou Fangyue Zhou Yuanyuan Gu Menglei Zhang Ganrong Zhang Fang Shen Keqin Hua Jingxin Ding |
| author_sort | Guannan Zhou |
| collection | DOAJ |
| description | As a common female reproductive system malignancy, cervical cancer (CC) disturbs numerous women’s health. This study demonstrates the role of the vaginal microbial environment (Peptostreptococcus anaerobius) in cervical cancer. Functional assays, including cell proliferation assay, tube formation assay, and immunofluorescence staining, revealed the effect of Peptostreptococcus anaerobius-treated macrophages on cell proliferation and the angiogenesis process. The tube formation assay disclosed the function of Peptostreptococcus anaerobius-treated macrophages on angiogenesis. In vivo assays were also established to explore the impact of Peptostreptococcus anaerobius-treated macrophages on tumor migration. The results revealed that Peptostreptococcus anaerobius-induced macrophages boosted cervical cancer migration and angiogenesis both in vitro and in vivo. Then, this study unveiled that Peptostreptococcus anaerobius-induced macrophage secreted VEGF to stimulate the angiogenesis in cervical cancer. As a whole, Peptostreptococcus anaerobius-induced macrophage facilitates cervical cancer development through modulation of VEGF expression. |
| format | Article |
| id | doaj-art-4935c7aee2644e5abd82d2f36e5952f2 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-4935c7aee2644e5abd82d2f36e5952f22025-02-03T01:22:52ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/3525735Vaginal Microbial Environment Skews Macrophage Polarization and Contributes to Cervical Cancer DevelopmentGuannan Zhou0Fangyue Zhou1Yuanyuan Gu2Menglei Zhang3Ganrong Zhang4Fang Shen5Keqin Hua6Jingxin Ding7Department of GynecologyThe International Peace Maternity and Child Health HospitalDepartment of GynecologyDepartment of GynecologyDepartment of GynecologyDepartment of GynecologyDepartment of GynecologyDepartment of GynecologyAs a common female reproductive system malignancy, cervical cancer (CC) disturbs numerous women’s health. This study demonstrates the role of the vaginal microbial environment (Peptostreptococcus anaerobius) in cervical cancer. Functional assays, including cell proliferation assay, tube formation assay, and immunofluorescence staining, revealed the effect of Peptostreptococcus anaerobius-treated macrophages on cell proliferation and the angiogenesis process. The tube formation assay disclosed the function of Peptostreptococcus anaerobius-treated macrophages on angiogenesis. In vivo assays were also established to explore the impact of Peptostreptococcus anaerobius-treated macrophages on tumor migration. The results revealed that Peptostreptococcus anaerobius-induced macrophages boosted cervical cancer migration and angiogenesis both in vitro and in vivo. Then, this study unveiled that Peptostreptococcus anaerobius-induced macrophage secreted VEGF to stimulate the angiogenesis in cervical cancer. As a whole, Peptostreptococcus anaerobius-induced macrophage facilitates cervical cancer development through modulation of VEGF expression.http://dx.doi.org/10.1155/2022/3525735 |
| spellingShingle | Guannan Zhou Fangyue Zhou Yuanyuan Gu Menglei Zhang Ganrong Zhang Fang Shen Keqin Hua Jingxin Ding Vaginal Microbial Environment Skews Macrophage Polarization and Contributes to Cervical Cancer Development Journal of Immunology Research |
| title | Vaginal Microbial Environment Skews Macrophage Polarization and Contributes to Cervical Cancer Development |
| title_full | Vaginal Microbial Environment Skews Macrophage Polarization and Contributes to Cervical Cancer Development |
| title_fullStr | Vaginal Microbial Environment Skews Macrophage Polarization and Contributes to Cervical Cancer Development |
| title_full_unstemmed | Vaginal Microbial Environment Skews Macrophage Polarization and Contributes to Cervical Cancer Development |
| title_short | Vaginal Microbial Environment Skews Macrophage Polarization and Contributes to Cervical Cancer Development |
| title_sort | vaginal microbial environment skews macrophage polarization and contributes to cervical cancer development |
| url | http://dx.doi.org/10.1155/2022/3525735 |
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