Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniens
IntroductionIn this study, we investigated the metabolic profiles of yak (Bos grunniens) follicles during the development period from the perspective of metabolomics, aiming to screen out the differential metabolites of yak follicles in different sizes and potential pathways during yak follicle deve...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1498703/full |
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| author | Jiuru Yan Jiuru Yan Jiuru Yan Yuxin Fu Yuxin Fu Yuxin Fu Lan Lan Huizhu Zhang Huizhu Zhang Huizhu Zhang Ling Huang Ling Huang Yaying Wang Yaying Wang Xianrong Xiong Xianrong Xiong Xianrong Xiong Shi Yin Shi Yin Shi Yin Jian Li Jian Li Jian Li Honghong He Honghong He Honghong He |
| author_facet | Jiuru Yan Jiuru Yan Jiuru Yan Yuxin Fu Yuxin Fu Yuxin Fu Lan Lan Huizhu Zhang Huizhu Zhang Huizhu Zhang Ling Huang Ling Huang Yaying Wang Yaying Wang Xianrong Xiong Xianrong Xiong Xianrong Xiong Shi Yin Shi Yin Shi Yin Jian Li Jian Li Jian Li Honghong He Honghong He Honghong He |
| author_sort | Jiuru Yan |
| collection | DOAJ |
| description | IntroductionIn this study, we investigated the metabolic profiles of yak (Bos grunniens) follicles during the development period from the perspective of metabolomics, aiming to screen out the differential metabolites of yak follicles in different sizes and potential pathways during yak follicle development and to provide a basis for the study of follicle development and developmental mechanisms in the further stage of development.MethodsA total of 20 four-year-old female yaks were selected, and follicles of different sizes were collected after slaughter and divided into d < 3 mm, 3–6 mm and d > 6 mm. The follicular fluid was collected, 6 replicates per group and subjected to LC–MS assay, combined with multidimensional and unidimensional statistical analyses to screen the differential metabolites between follicles of different sizes. Differential metabolites enriched KEGG pathways to screen the pathways that might be related to follicle development.ResultsWe found that most of the metabolites were mainly enriched in amino acid metabolism pathways, energy metabolism pathways and other pathways of cofactor synthesis, and that during the development of the small follicle to the large follicle, 2-Lysophosphatidylcholine, PC (17:0/0:0), PC (16:0/0:0), and LysoPC (18:0/0:0) were down-regulated; Dioctyl succinate, P-Coumaraldehyde, ISOPRENE, L-Isoleucine, Dioctyl succinate up-regulated.ConclusionThese results suggest that amino acid metabolism, the production of steroid hormones and their metabolites, and the metabolic activity of granulosa cells play important roles in follicle development. The results provide a theoretical basis for further exploration of follicular development in yak. |
| format | Article |
| id | doaj-art-491bda9818944ba28ccc50ec0e8dae10 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-491bda9818944ba28ccc50ec0e8dae102025-01-28T06:41:21ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011210.3389/fvets.2025.14987031498703Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniensJiuru Yan0Jiuru Yan1Jiuru Yan2Yuxin Fu3Yuxin Fu4Yuxin Fu5Lan Lan6Huizhu Zhang7Huizhu Zhang8Huizhu Zhang9Ling Huang10Ling Huang11Yaying Wang12Yaying Wang13Xianrong Xiong14Xianrong Xiong15Xianrong Xiong16Shi Yin17Shi Yin18Shi Yin19Jian Li20Jian Li21Jian Li22Honghong He23Honghong He24Honghong He25Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaAnimal Husbandry Science Institute of Ganzi Tibetan Autonomous Prefecture, Kangding, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Chengdu, ChinaCollege of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, ChinaKey Laboratory of Animal Medicine, Southwest Minzu University, Chengdu, ChinaIntroductionIn this study, we investigated the metabolic profiles of yak (Bos grunniens) follicles during the development period from the perspective of metabolomics, aiming to screen out the differential metabolites of yak follicles in different sizes and potential pathways during yak follicle development and to provide a basis for the study of follicle development and developmental mechanisms in the further stage of development.MethodsA total of 20 four-year-old female yaks were selected, and follicles of different sizes were collected after slaughter and divided into d < 3 mm, 3–6 mm and d > 6 mm. The follicular fluid was collected, 6 replicates per group and subjected to LC–MS assay, combined with multidimensional and unidimensional statistical analyses to screen the differential metabolites between follicles of different sizes. Differential metabolites enriched KEGG pathways to screen the pathways that might be related to follicle development.ResultsWe found that most of the metabolites were mainly enriched in amino acid metabolism pathways, energy metabolism pathways and other pathways of cofactor synthesis, and that during the development of the small follicle to the large follicle, 2-Lysophosphatidylcholine, PC (17:0/0:0), PC (16:0/0:0), and LysoPC (18:0/0:0) were down-regulated; Dioctyl succinate, P-Coumaraldehyde, ISOPRENE, L-Isoleucine, Dioctyl succinate up-regulated.ConclusionThese results suggest that amino acid metabolism, the production of steroid hormones and their metabolites, and the metabolic activity of granulosa cells play important roles in follicle development. The results provide a theoretical basis for further exploration of follicular development in yak.https://www.frontiersin.org/articles/10.3389/fvets.2025.1498703/fullfollicular developmentmetabolomicsgranulosa cellsovaryBos grunniens |
| spellingShingle | Jiuru Yan Jiuru Yan Jiuru Yan Yuxin Fu Yuxin Fu Yuxin Fu Lan Lan Huizhu Zhang Huizhu Zhang Huizhu Zhang Ling Huang Ling Huang Yaying Wang Yaying Wang Xianrong Xiong Xianrong Xiong Xianrong Xiong Shi Yin Shi Yin Shi Yin Jian Li Jian Li Jian Li Honghong He Honghong He Honghong He Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniens Frontiers in Veterinary Science follicular development metabolomics granulosa cells ovary Bos grunniens |
| title | Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniens |
| title_full | Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniens |
| title_fullStr | Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniens |
| title_full_unstemmed | Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniens |
| title_short | Correlation between follicle diameter and pre-ovulatory metabolic profile in Bos grunniens |
| title_sort | correlation between follicle diameter and pre ovulatory metabolic profile in bos grunniens |
| topic | follicular development metabolomics granulosa cells ovary Bos grunniens |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1498703/full |
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