Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells
Background and Objective. ATP-sensitive potassium (KATP) channel couples cell metabolism to excitability. To explore role of KATP channels in cellular photobiomodulation, we designed experiment to study effect of low intensity 808 nm laser irradiation on the activity of membrane KATP channel. Study...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Photoenergy |
| Online Access: | http://dx.doi.org/10.1155/2014/898752 |
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| _version_ | 1832558312206368768 |
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| author | Fu-qing Zhong Yang Li Xian-qiang Mi |
| author_facet | Fu-qing Zhong Yang Li Xian-qiang Mi |
| author_sort | Fu-qing Zhong |
| collection | DOAJ |
| description | Background and Objective. ATP-sensitive potassium (KATP) channel couples cell metabolism to excitability. To explore role of KATP channels in cellular photobiomodulation, we designed experiment to study effect of low intensity 808 nm laser irradiation on the activity of membrane KATP channel. Study Design/Materials and Methods. Plasmids encoding Kir6.2 was constructed and heterologously expressed in cultured mammalian HEK-293 cells. The patch-clamp and data acquisition systems were used to record KATP channel current before and after irradiation. A laser beam of Ga-As 808 nm at 5 mW/cm2 was used in experiments. A one-way ANOVA test followed by a post hoc Student-Newman-Keuls test was used to assess the statistical differences between data groups. Results. Obvious openings of KATP channels of Kir6.2-transfected HEK-293 cells and excised patches were recorded during and after low intensity 808 nm laser irradiation. Compared with the channels that did not undergo irradiation, open probability, current amplitude, and dwell time of KATP channels after irradiation improved. Conclusions. Low intensity 808 nm laser irradiation may activate membrane KATP channels of Kir6.2-transfected HEK-293 cells and in excised patches. |
| format | Article |
| id | doaj-art-490e8c57ee004b5eb28af9ffd5a02849 |
| institution | Kabale University |
| issn | 1110-662X 1687-529X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Photoenergy |
| spelling | doaj-art-490e8c57ee004b5eb28af9ffd5a028492025-02-03T01:32:36ZengWileyInternational Journal of Photoenergy1110-662X1687-529X2014-01-01201410.1155/2014/898752898752Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 CellsFu-qing Zhong0Yang Li1Xian-qiang Mi2College of Medical Device and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaCAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, ChinaCollege of Medical Device and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaBackground and Objective. ATP-sensitive potassium (KATP) channel couples cell metabolism to excitability. To explore role of KATP channels in cellular photobiomodulation, we designed experiment to study effect of low intensity 808 nm laser irradiation on the activity of membrane KATP channel. Study Design/Materials and Methods. Plasmids encoding Kir6.2 was constructed and heterologously expressed in cultured mammalian HEK-293 cells. The patch-clamp and data acquisition systems were used to record KATP channel current before and after irradiation. A laser beam of Ga-As 808 nm at 5 mW/cm2 was used in experiments. A one-way ANOVA test followed by a post hoc Student-Newman-Keuls test was used to assess the statistical differences between data groups. Results. Obvious openings of KATP channels of Kir6.2-transfected HEK-293 cells and excised patches were recorded during and after low intensity 808 nm laser irradiation. Compared with the channels that did not undergo irradiation, open probability, current amplitude, and dwell time of KATP channels after irradiation improved. Conclusions. Low intensity 808 nm laser irradiation may activate membrane KATP channels of Kir6.2-transfected HEK-293 cells and in excised patches.http://dx.doi.org/10.1155/2014/898752 |
| spellingShingle | Fu-qing Zhong Yang Li Xian-qiang Mi Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells International Journal of Photoenergy |
| title | Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells |
| title_full | Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells |
| title_fullStr | Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells |
| title_full_unstemmed | Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells |
| title_short | Photobiomodulation on KATP Channels of Kir6.2-Transfected HEK-293 Cells |
| title_sort | photobiomodulation on katp channels of kir6 2 transfected hek 293 cells |
| url | http://dx.doi.org/10.1155/2014/898752 |
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