In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible Fungi
Protein-polysaccharide complexes of Agaricus bisporus, Stropharia rugosa and Morchella esculenta were prepared and their protein and polysaccharide contents as well as amino acid and monosaccharide composition were evaluated. The in vitro and in vivo lipid-lowering effects of the complexes on oleic...
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China Food Publishing Company
2024-12-01
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-014.pdf |
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| author | GAO Chen, LI Wen, CHEN Wanchao, WU Di, ZHANG Zhong, LIU Peng, XIA Yongjun, YANG Yan |
| author_facet | GAO Chen, LI Wen, CHEN Wanchao, WU Di, ZHANG Zhong, LIU Peng, XIA Yongjun, YANG Yan |
| author_sort | GAO Chen, LI Wen, CHEN Wanchao, WU Di, ZHANG Zhong, LIU Peng, XIA Yongjun, YANG Yan |
| collection | DOAJ |
| description | Protein-polysaccharide complexes of Agaricus bisporus, Stropharia rugosa and Morchella esculenta were prepared and their protein and polysaccharide contents as well as amino acid and monosaccharide composition were evaluated. The in vitro and in vivo lipid-lowering effects of the complexes on oleic acid-induced liver LO-2 cells and high-fat diet fed mice were investigated. The results showed that the protein contents of the three complexes were (55.56 ± 0.51)%, (52.41 ± 0.31)%, and (54.96 ± 0.47)%, respectively, and the polysaccharide contents were (21.98 ± 1.17)%, (31.95 ± 0.52)%, and (15.55 ± 0.78)%, respectively. In vitro cell experiments showed that all the complexes significantly inhibited the accumulation of lipid droplets in hepatocytes. Animal experiments showed that they significantly inhibited the increase in body mass, triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) levels, blocked the swelling of the swelling of hepatocytes, and inhibited hepatic lipid deposition in mice fed a high-fat diet. In the high-dose group, the hepatic lipid content of mice with hyperlipidemia was reduced from (30.59 ± 0.38)% to (13.74 ± 1.57)%, (9.68 ± 0.73)%, and (7.15 ± 0.39)%, respectively, by the complexes from A. bisporus, S. rugosa and M. esculenta, and the inhibition rates of hepatic lipid droplets by these complexes were (55.09 ± 5.12)%, (68.34 ± 2.37)% and (76.64 ± 1.28)%, respectively. In addition, the protein-polysaccharide complexes alleviated liver function damage caused by long-term high-fat diet. In summary, it can be concluded that A. bisporus, S. rugosa and M. esculenta protein-polysaccharide complexes can regulate lipid metabolism, alleviate liver injury and hyperlipidemia, which provides a reference for the development of functional products based on edible fungi. |
| format | Article |
| id | doaj-art-4907ac0c56ae4441baa177897c0b2c94 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-4907ac0c56ae4441baa177897c0b2c942025-02-05T09:08:01ZengChina Food Publishing CompanyShipin Kexue1002-66302024-12-01452411712710.7506/spkx1002-6630-20240524-202In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible FungiGAO Chen, LI Wen, CHEN Wanchao, WU Di, ZHANG Zhong, LIU Peng, XIA Yongjun, YANG Yan0(1. School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; 2. Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture and Rural Affairs, National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China)Protein-polysaccharide complexes of Agaricus bisporus, Stropharia rugosa and Morchella esculenta were prepared and their protein and polysaccharide contents as well as amino acid and monosaccharide composition were evaluated. The in vitro and in vivo lipid-lowering effects of the complexes on oleic acid-induced liver LO-2 cells and high-fat diet fed mice were investigated. The results showed that the protein contents of the three complexes were (55.56 ± 0.51)%, (52.41 ± 0.31)%, and (54.96 ± 0.47)%, respectively, and the polysaccharide contents were (21.98 ± 1.17)%, (31.95 ± 0.52)%, and (15.55 ± 0.78)%, respectively. In vitro cell experiments showed that all the complexes significantly inhibited the accumulation of lipid droplets in hepatocytes. Animal experiments showed that they significantly inhibited the increase in body mass, triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) levels, blocked the swelling of the swelling of hepatocytes, and inhibited hepatic lipid deposition in mice fed a high-fat diet. In the high-dose group, the hepatic lipid content of mice with hyperlipidemia was reduced from (30.59 ± 0.38)% to (13.74 ± 1.57)%, (9.68 ± 0.73)%, and (7.15 ± 0.39)%, respectively, by the complexes from A. bisporus, S. rugosa and M. esculenta, and the inhibition rates of hepatic lipid droplets by these complexes were (55.09 ± 5.12)%, (68.34 ± 2.37)% and (76.64 ± 1.28)%, respectively. In addition, the protein-polysaccharide complexes alleviated liver function damage caused by long-term high-fat diet. In summary, it can be concluded that A. bisporus, S. rugosa and M. esculenta protein-polysaccharide complexes can regulate lipid metabolism, alleviate liver injury and hyperlipidemia, which provides a reference for the development of functional products based on edible fungi.https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-014.pdfedible fungi; protein; polysaccharide; hypolipidemia |
| spellingShingle | GAO Chen, LI Wen, CHEN Wanchao, WU Di, ZHANG Zhong, LIU Peng, XIA Yongjun, YANG Yan In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible Fungi Shipin Kexue edible fungi; protein; polysaccharide; hypolipidemia |
| title | In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible Fungi |
| title_full | In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible Fungi |
| title_fullStr | In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible Fungi |
| title_full_unstemmed | In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible Fungi |
| title_short | In Vitro and in Vivo Hypolipidemic Effects of Protein-Polysaccharide Complexes from Three Species of Edible Fungi |
| title_sort | in vitro and in vivo hypolipidemic effects of protein polysaccharide complexes from three species of edible fungi |
| topic | edible fungi; protein; polysaccharide; hypolipidemia |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2024-45-24-014.pdf |
| work_keys_str_mv | AT gaochenliwenchenwanchaowudizhangzhongliupengxiayongjunyangyan invitroandinvivohypolipidemiceffectsofproteinpolysaccharidecomplexesfromthreespeciesofediblefungi |