A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae
Plant growth-promoting bacteria (PGPB) have emerged as sustainable tool for managing plant diseases. This study investigates the potential of a Pseudomonas-based biocontrol agent to manage bacterial blight (BB) in pomegranate. This major disease is caused by Xanthomonas axonopodis pv. punicae (Xap)...
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Elsevier
2025-02-01
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| Series: | Biological Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1049964424002512 |
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| author | Pavan Kumar Giandomenico Corrado Girigowda Manjunatha Suma Ramegowda Pradeep Kumar Boris Basile Ganadalu Puttaswamy Mutthuraju Baggana Girish Jasmitha |
| author_facet | Pavan Kumar Giandomenico Corrado Girigowda Manjunatha Suma Ramegowda Pradeep Kumar Boris Basile Ganadalu Puttaswamy Mutthuraju Baggana Girish Jasmitha |
| author_sort | Pavan Kumar |
| collection | DOAJ |
| description | Plant growth-promoting bacteria (PGPB) have emerged as sustainable tool for managing plant diseases. This study investigates the potential of a Pseudomonas-based biocontrol agent to manage bacterial blight (BB) in pomegranate. This major disease is caused by Xanthomonas axonopodis pv. punicae (Xap) and it is traditionally controlled with antibiotics. Of the 151 bacterial isolates obtained from the pomegranate rhizosphere, three (UHSPS15A, UHSPS33, and UHSPS54) demonstrated the strongest inhibitory effects against Xap in vitro, and their identification as Pseudomonas was confirmed through DNA analysis. Greenhouse trials with Xap-inoculated plants revealed that preventive application of each of the three isolates was more effective than curative, with UHSPS15A providing the highest protection. A talc-based formulation was developed using UHSPS15A. After evaluating its stability and efficacy in greenhouse Xap-inoculated pomegranates plants, open-fields trials indicated that among the three different treatment modes tested, the combined soil and foliar application achieved the highest disease protection and fruit yield, topping the standard antibiotic control. These findings recommend that integrating Pseudomonas-based bio-formulations into disease management strategies could significantly reduce reliance on synthetic chemicals, offering a sustainable alternative for controlling BB in pomegranate. |
| format | Article |
| id | doaj-art-48eefc72f0a44b6493ac4eddea3c1e61 |
| institution | Kabale University |
| issn | 1049-9644 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biological Control |
| spelling | doaj-art-48eefc72f0a44b6493ac4eddea3c1e612025-02-05T04:31:09ZengElsevierBiological Control1049-96442025-02-01201105686A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicaePavan Kumar0Giandomenico Corrado1Girigowda Manjunatha2Suma Ramegowda3Pradeep Kumar4Boris Basile5Ganadalu Puttaswamy Mutthuraju6Baggana Girish Jasmitha7Research and Development Division, Sea6 Energy Private Limited, Centre for Cellular and Molecular Platforms, NCBS-TIFR Campus, Bengaluru, India; College of Horticulture, Mysore, University of Horticultural Sciences, Bagalkot 587104, IndiaDepartment of Agricultural Sciences, University of Naples Federico II, 80055, Portici, ItalyCollege of Horticulture, Mysore, University of Horticultural Sciences, Bagalkot 587104, India; Corresponding authors.College of Agriculture, Mandya, University of Agricultural Sciences, Bangalore, IndiaICAR-Central Arid Zone Research Institute, Jodhpur 342003, IndiaDepartment of Agricultural Sciences, University of Naples Federico II, 80055, Portici, Italy; Corresponding authors.College of Horticulture, Mysore, University of Horticultural Sciences, Bagalkot 587104, IndiaCollege of Horticulture, Mysore, University of Horticultural Sciences, Bagalkot 587104, IndiaPlant growth-promoting bacteria (PGPB) have emerged as sustainable tool for managing plant diseases. This study investigates the potential of a Pseudomonas-based biocontrol agent to manage bacterial blight (BB) in pomegranate. This major disease is caused by Xanthomonas axonopodis pv. punicae (Xap) and it is traditionally controlled with antibiotics. Of the 151 bacterial isolates obtained from the pomegranate rhizosphere, three (UHSPS15A, UHSPS33, and UHSPS54) demonstrated the strongest inhibitory effects against Xap in vitro, and their identification as Pseudomonas was confirmed through DNA analysis. Greenhouse trials with Xap-inoculated plants revealed that preventive application of each of the three isolates was more effective than curative, with UHSPS15A providing the highest protection. A talc-based formulation was developed using UHSPS15A. After evaluating its stability and efficacy in greenhouse Xap-inoculated pomegranates plants, open-fields trials indicated that among the three different treatment modes tested, the combined soil and foliar application achieved the highest disease protection and fruit yield, topping the standard antibiotic control. These findings recommend that integrating Pseudomonas-based bio-formulations into disease management strategies could significantly reduce reliance on synthetic chemicals, offering a sustainable alternative for controlling BB in pomegranate.http://www.sciencedirect.com/science/article/pii/S1049964424002512Punica granatumPlant growth-promoting bacteriaSustainable disease managementBiological controlYield |
| spellingShingle | Pavan Kumar Giandomenico Corrado Girigowda Manjunatha Suma Ramegowda Pradeep Kumar Boris Basile Ganadalu Puttaswamy Mutthuraju Baggana Girish Jasmitha A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae Biological Control Punica granatum Plant growth-promoting bacteria Sustainable disease management Biological control Yield |
| title | A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae |
| title_full | A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae |
| title_fullStr | A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae |
| title_full_unstemmed | A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae |
| title_short | A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae |
| title_sort | pseudomonas based bio formulation to control bacterial blight of pomegranate caused by xanthomonas axonopodis pv punicae |
| topic | Punica granatum Plant growth-promoting bacteria Sustainable disease management Biological control Yield |
| url | http://www.sciencedirect.com/science/article/pii/S1049964424002512 |
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