Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings
Early onset periodontitis (EOP) is a chronic inflammatory periodontal disease with a strong genetic link affecting individuals aged 17 to 25. In the familial studies we tested the hypothesis about the role of Th1 and Th2 cytokines in the pathogenesis of EOP disease. The study involved 6 individuals...
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/096293500411587 |
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| author | Jiřina Bártová Zuzana Krátká-Opatrná Jarmila Procházková Otakar Krejsa Jana Dušková Lubor Mrklas Helena Tlaskalová Božena Cukrowská |
| author_facet | Jiřina Bártová Zuzana Krátká-Opatrná Jarmila Procházková Otakar Krejsa Jana Dušková Lubor Mrklas Helena Tlaskalová Božena Cukrowská |
| author_sort | Jiřina Bártová |
| collection | DOAJ |
| description | Early onset periodontitis (EOP) is a chronic inflammatory periodontal disease with a strong genetic link affecting individuals aged 17 to 25. In the familial studies we tested the hypothesis about the role of Th1 and Th2 cytokines in the pathogenesis of EOP disease. The study involved 6 individuals with EOP disease and their 6 siblings with healthy periodontium. Actinobacillus actinomycetemcomitans (A. a.), a bacterium typical for EOP, was detected in all people studied. Th1 and Th2 cytokine production was measured after in vitro stimulation. Peripheral blood mononuclear cells (PBMC) were isolated and cultivated for 24 h and 7 days with PWM, A. a. or Escherichia coli. The levels of IL–4, IFN-gamma, IgA, IgG and IgM were measured by ELISA methods. After in vitro stimulation of PBMC, a significantly higher production of IL–4 and significantly lower production of IFN-gamma were found in the group of patients compared with their healthy siblings. The increased level of IL–4 in patients was in good agreement with an increased level of IgM after stimulation of lymphocytes with E. coli. These results support Seymour’s hypothesis according to which patients with progressive disease primarily activate Th2 lymphocytes while non-susceptible individuals activate Th1 lymphocytes. |
| format | Article |
| id | doaj-art-48c6f7e75bc244d8b8203fb3a690f0f0 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-48c6f7e75bc244d8b8203fb3a690f0f02025-02-03T01:27:16ZengWileyMediators of Inflammation0962-93511466-18612000-01-019211512010.1080/096293500411587Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblingsJiřina Bártová0Zuzana Krátká-Opatrná1Jarmila Procházková2Otakar Krejsa3Jana Dušková4Lubor Mrklas5Helena Tlaskalová6Božena Cukrowská7Institute of Dental Research, Vinohradská 48, Prague 2 12060, Czech RepublicInstitute of Dental Research, Vinohradská 48, Prague 2 12060, Czech RepublicInstitute of Dental Research, Vinohradská 48, Prague 2 12060, Czech RepublicInstitute of Dental Research, Vinohradská 48, Prague 2 12060, Czech RepublicInstitute of Dental Research, Vinohradská 48, Prague 2 12060, Czech RepublicInstitute of Dental Research, Vinohradská 48, Prague 2 12060, Czech RepublicInstitute of Microbiology, Czech Academy of Sciences, Videňská 1083, Prague 14000, Czech RepublicInstitute of Microbiology, Czech Academy of Sciences, Videňská 1083, Prague 14000, Czech RepublicEarly onset periodontitis (EOP) is a chronic inflammatory periodontal disease with a strong genetic link affecting individuals aged 17 to 25. In the familial studies we tested the hypothesis about the role of Th1 and Th2 cytokines in the pathogenesis of EOP disease. The study involved 6 individuals with EOP disease and their 6 siblings with healthy periodontium. Actinobacillus actinomycetemcomitans (A. a.), a bacterium typical for EOP, was detected in all people studied. Th1 and Th2 cytokine production was measured after in vitro stimulation. Peripheral blood mononuclear cells (PBMC) were isolated and cultivated for 24 h and 7 days with PWM, A. a. or Escherichia coli. The levels of IL–4, IFN-gamma, IgA, IgG and IgM were measured by ELISA methods. After in vitro stimulation of PBMC, a significantly higher production of IL–4 and significantly lower production of IFN-gamma were found in the group of patients compared with their healthy siblings. The increased level of IL–4 in patients was in good agreement with an increased level of IgM after stimulation of lymphocytes with E. coli. These results support Seymour’s hypothesis according to which patients with progressive disease primarily activate Th2 lymphocytes while non-susceptible individuals activate Th1 lymphocytes.http://dx.doi.org/10.1080/096293500411587 |
| spellingShingle | Jiřina Bártová Zuzana Krátká-Opatrná Jarmila Procházková Otakar Krejsa Jana Dušková Lubor Mrklas Helena Tlaskalová Božena Cukrowská Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings Mediators of Inflammation |
| title | Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings |
| title_full | Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings |
| title_fullStr | Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings |
| title_full_unstemmed | Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings |
| title_short | Th1 and Th2 cytokine profile in patients with early onset periodontitis and their healthy siblings |
| title_sort | th1 and th2 cytokine profile in patients with early onset periodontitis and their healthy siblings |
| url | http://dx.doi.org/10.1080/096293500411587 |
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