In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions
Abstract Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces a novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specificall...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Discover Nano |
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| Online Access: | https://doi.org/10.1186/s11671-025-04183-0 |
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| author | Hari Prasath Nagaiah Malik Basha Samsudeen Akshaya Rani Augustus Karutha Pandian Shunmugiah |
| author_facet | Hari Prasath Nagaiah Malik Basha Samsudeen Akshaya Rani Augustus Karutha Pandian Shunmugiah |
| author_sort | Hari Prasath Nagaiah |
| collection | DOAJ |
| description | Abstract Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces a novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specifically designed to enhance antimicrobial activity and promote wound healing. The nanocomposite was thoroughly characterized using advanced analytical techniques, confirming its nanoscale structure, stability and chemical composition. The Ag+ZnO+EU nanocomposite demonstrated potent antimicrobial efficacy against a range of wound associated pathogens, including standard and clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Minimum inhibitory concentrations of Ag+ZnO+EU for standard and clinical isolates were significantly lower than those of the individual components, highlighting the synergistic effect of the nanocomposite. Time-kill assays revealed rapid microbial eradication, achieving complete sterility within 240-min. Importantly, the nanocomposite effectively eliminated persister-like cells, which are typically resistant to conventional treatments, suggesting a potential solution for persistent infections. In vitro scratch assays using human keratinocyte cells demonstrated that the Ag+ZnO+EU nanocomposite significantly accelerated wound closure, with near-complete healing observed within 24-h, indicating enhanced cell migration and tissue regeneration. Additionally, the nanocomposite showed potential antidiabetic effects by increasing glucose uptake up to 97.21% in an in vitro assay using 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose, a fluorescent glucose analog, suggesting potential applications beyond wound healing. These findings highlight the Ag+ZnO+EU nanocomposite as a promising candidate for addressing both antimicrobial resistance and impaired wound healing in diabetic contexts. Graphical Abstract |
| format | Article |
| id | doaj-art-48bf855b57b346b6b95e99e6e5881e8a |
| institution | Kabale University |
| issn | 2731-9229 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Nano |
| spelling | doaj-art-48bf855b57b346b6b95e99e6e5881e8a2025-01-26T12:48:46ZengSpringerDiscover Nano2731-92292025-01-0120113010.1186/s11671-025-04183-0In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditionsHari Prasath Nagaiah0Malik Basha Samsudeen1Akshaya Rani Augustus2Karutha Pandian Shunmugiah3Department of Biotechnology, Alagappa UniversityDepartment of Biotechnology, Alagappa UniversityDepartment of Biotechnology, Alagappa UniversityDepartment of Biotechnology, Alagappa UniversityAbstract Diabetic wounds with chronic infections present a significant challenge, exacerbated by the growing issue of antimicrobial resistance, which often leads to delayed healing and increased morbidity. This study introduces a novel silver-zinc oxide-eugenol (Ag+ZnO+EU) nanocomposite, specifically designed to enhance antimicrobial activity and promote wound healing. The nanocomposite was thoroughly characterized using advanced analytical techniques, confirming its nanoscale structure, stability and chemical composition. The Ag+ZnO+EU nanocomposite demonstrated potent antimicrobial efficacy against a range of wound associated pathogens, including standard and clinical isolates of Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Minimum inhibitory concentrations of Ag+ZnO+EU for standard and clinical isolates were significantly lower than those of the individual components, highlighting the synergistic effect of the nanocomposite. Time-kill assays revealed rapid microbial eradication, achieving complete sterility within 240-min. Importantly, the nanocomposite effectively eliminated persister-like cells, which are typically resistant to conventional treatments, suggesting a potential solution for persistent infections. In vitro scratch assays using human keratinocyte cells demonstrated that the Ag+ZnO+EU nanocomposite significantly accelerated wound closure, with near-complete healing observed within 24-h, indicating enhanced cell migration and tissue regeneration. Additionally, the nanocomposite showed potential antidiabetic effects by increasing glucose uptake up to 97.21% in an in vitro assay using 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose, a fluorescent glucose analog, suggesting potential applications beyond wound healing. These findings highlight the Ag+ZnO+EU nanocomposite as a promising candidate for addressing both antimicrobial resistance and impaired wound healing in diabetic contexts. Graphical Abstracthttps://doi.org/10.1186/s11671-025-04183-0Antimicrobial resistanceWound healingDiabetic woundsChronic infectionsGlucose uptake |
| spellingShingle | Hari Prasath Nagaiah Malik Basha Samsudeen Akshaya Rani Augustus Karutha Pandian Shunmugiah In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions Discover Nano Antimicrobial resistance Wound healing Diabetic wounds Chronic infections Glucose uptake |
| title | In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions |
| title_full | In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions |
| title_fullStr | In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions |
| title_full_unstemmed | In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions |
| title_short | In vitro evaluation of silver-zinc oxide-eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions |
| title_sort | in vitro evaluation of silver zinc oxide eugenol nanocomposite for enhanced antimicrobial and wound healing applications in diabetic conditions |
| topic | Antimicrobial resistance Wound healing Diabetic wounds Chronic infections Glucose uptake |
| url | https://doi.org/10.1186/s11671-025-04183-0 |
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