Control of Opportunistic Oral Cavity Infections Using Postbiotics Secreted by Aerobic Oral Flora, with Minimal Impact on Host Cells
Introduction: Postbiotics derived from the natural oral microbiome offer a potential solution for chemotherapy-induced oral dysbiosis by restoring microbial balance. These compounds hold promise for managing oral infections, particularly in vulnerable populations, by restoring microbial balance,...
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2024-09-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| Online Access: | https://jommid.pasteur.ac.ir/article-1-687-en.html |
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| author | Haranoush Ghafouri1 Parnian Mashayekhi1 Maryam Davoudi1* |
| author_facet | Haranoush Ghafouri1 Parnian Mashayekhi1 Maryam Davoudi1* |
| author_sort | Haranoush Ghafouri1 |
| collection | DOAJ |
| description | Introduction: Postbiotics derived from the natural oral microbiome offer a
potential solution for chemotherapy-induced oral dysbiosis by restoring
microbial balance. These compounds hold promise for managing oral
infections, particularly in vulnerable populations, by restoring microbial
balance, directly inhibiting pathogen growth, and promoting a healthy
mucosal immune response. This study evaluates the antimicrobial activity of
postbiotics derived from aerobic oral bacteria against Staphylococcus aureus
and assesses their cytotoxicity in human cells, contributing to the
development of new therapeutic strategies for oral infections. Methods:
Specific strains of aerobic oral bacteria were isolated and cultured in nutrient
broth at 37°C in a 5% CO2 atmosphere. After 24 h of incubation, the
supernatant containing postbiotics was collected and centrifuged, followed
by filtration to isolate the postbiotics. The antibacterial activity of these
postbiotics was assessed against S. aureus isolated from oral wounds using
the agar well diffusion method on Mueller-Hinton agar plates. In parallel,
the cytotoxic effects on Normal Human Dermal Fibroblasts (NHDF) were
evaluated through MTT viability assays to measure metabolic activity, and
SYBR Green staining to quantify DNA content, and assess cell membrane
integrity. Statistical analysis was performed using one-way ANOVA
followed by Tukey's post-hoc test, with significance set at P < 0.05. Results:
Postbiotics exhibited significant antimicrobial activity against S. aureus, as
evidenced by a mean zone of inhibition of 17.0 mm (±1.4 mm), while no
inhibition was observed in saline controls. Furthermore, postbiotics
treatment resulted in NHDF cell viability of 94% (±1.6%) compared to the
untreated controls 75.2% (±0.7%); P < 0.0001, demonstrating their
biocompatibility. Conclusion: This study demonstrates the promising
therapeutic potential of postbiotics for combating opportunistic oral
infections, exhibiting negligible cytotoxicity towards host cells. Future
research will explore isolating and identifying the active components of
these postbiotics and evaluating their efficacy in vivo models and human oral
cell lines. |
| format | Article |
| id | doaj-art-48a24ae2ce6943f1b9f21e633e7bd54f |
| institution | OA Journals |
| issn | 2345-5349 2345-5330 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj-art-48a24ae2ce6943f1b9f21e633e7bd54f2025-08-20T02:15:17ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302024-09-0112321722310.61186/JoMMID.12.3.217Control of Opportunistic Oral Cavity Infections Using Postbiotics Secreted by Aerobic Oral Flora, with Minimal Impact on Host CellsHaranoush Ghafouri10https://orcid.org/0009-0008-0546-6703Parnian Mashayekhi11https://orcid.org/0009-0002-0540-9393Maryam Davoudi1*2https://orcid.org/0000-0002-1048-12371Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran1Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran1Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, IranIntroduction: Postbiotics derived from the natural oral microbiome offer a potential solution for chemotherapy-induced oral dysbiosis by restoring microbial balance. These compounds hold promise for managing oral infections, particularly in vulnerable populations, by restoring microbial balance, directly inhibiting pathogen growth, and promoting a healthy mucosal immune response. This study evaluates the antimicrobial activity of postbiotics derived from aerobic oral bacteria against Staphylococcus aureus and assesses their cytotoxicity in human cells, contributing to the development of new therapeutic strategies for oral infections. Methods: Specific strains of aerobic oral bacteria were isolated and cultured in nutrient broth at 37°C in a 5% CO2 atmosphere. After 24 h of incubation, the supernatant containing postbiotics was collected and centrifuged, followed by filtration to isolate the postbiotics. The antibacterial activity of these postbiotics was assessed against S. aureus isolated from oral wounds using the agar well diffusion method on Mueller-Hinton agar plates. In parallel, the cytotoxic effects on Normal Human Dermal Fibroblasts (NHDF) were evaluated through MTT viability assays to measure metabolic activity, and SYBR Green staining to quantify DNA content, and assess cell membrane integrity. Statistical analysis was performed using one-way ANOVA followed by Tukey's post-hoc test, with significance set at P < 0.05. Results: Postbiotics exhibited significant antimicrobial activity against S. aureus, as evidenced by a mean zone of inhibition of 17.0 mm (±1.4 mm), while no inhibition was observed in saline controls. Furthermore, postbiotics treatment resulted in NHDF cell viability of 94% (±1.6%) compared to the untreated controls 75.2% (±0.7%); P < 0.0001, demonstrating their biocompatibility. Conclusion: This study demonstrates the promising therapeutic potential of postbiotics for combating opportunistic oral infections, exhibiting negligible cytotoxicity towards host cells. Future research will explore isolating and identifying the active components of these postbiotics and evaluating their efficacy in vivo models and human oral cell lines.https://jommid.pasteur.ac.ir/article-1-687-en.htmlpostbioticsopportunistic infectionsoral microbiomeoral cavitystaphylococcus aureusagar well diffusionmtt assaysybr green staining |
| spellingShingle | Haranoush Ghafouri1 Parnian Mashayekhi1 Maryam Davoudi1* Control of Opportunistic Oral Cavity Infections Using Postbiotics Secreted by Aerobic Oral Flora, with Minimal Impact on Host Cells Journal of Medical Microbiology and Infectious Diseases postbiotics opportunistic infections oral microbiome oral cavity staphylococcus aureus agar well diffusion mtt assay sybr green staining |
| title | Control of Opportunistic Oral Cavity Infections Using Postbiotics Secreted by Aerobic Oral Flora, with Minimal Impact on Host Cells |
| title_full | Control of Opportunistic Oral Cavity Infections Using Postbiotics Secreted by Aerobic Oral Flora, with Minimal Impact on Host Cells |
| title_fullStr | Control of Opportunistic Oral Cavity Infections Using Postbiotics Secreted by Aerobic Oral Flora, with Minimal Impact on Host Cells |
| title_full_unstemmed | Control of Opportunistic Oral Cavity Infections Using Postbiotics Secreted by Aerobic Oral Flora, with Minimal Impact on Host Cells |
| title_short | Control of Opportunistic Oral Cavity Infections Using Postbiotics Secreted by Aerobic Oral Flora, with Minimal Impact on Host Cells |
| title_sort | control of opportunistic oral cavity infections using postbiotics secreted by aerobic oral flora with minimal impact on host cells |
| topic | postbiotics opportunistic infections oral microbiome oral cavity staphylococcus aureus agar well diffusion mtt assay sybr green staining |
| url | https://jommid.pasteur.ac.ir/article-1-687-en.html |
| work_keys_str_mv | AT haranoushghafouri1 controlofopportunisticoralcavityinfectionsusingpostbioticssecretedbyaerobicoralflorawithminimalimpactonhostcells AT parnianmashayekhi1 controlofopportunisticoralcavityinfectionsusingpostbioticssecretedbyaerobicoralflorawithminimalimpactonhostcells AT maryamdavoudi1 controlofopportunisticoralcavityinfectionsusingpostbioticssecretedbyaerobicoralflorawithminimalimpactonhostcells |