Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes
Telomeres, the protective DNA-protein complexes at the ends of linear chromosomes, are important for genome stability. Leukocyte or peripheral blood mononuclear cell (PBMC) telomere length is a potential biomarker for human aging that integrates genetic, environmental, and lifestyle factors and is a...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/5371050 |
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| _version_ | 1832566671981674496 |
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| author | Jue Lin Joshua Cheon Rashida Brown Michael Coccia Eli Puterman Kirstin Aschbacher Elizabeth Sinclair Elissa Epel Elizabeth H. Blackburn |
| author_facet | Jue Lin Joshua Cheon Rashida Brown Michael Coccia Eli Puterman Kirstin Aschbacher Elizabeth Sinclair Elissa Epel Elizabeth H. Blackburn |
| author_sort | Jue Lin |
| collection | DOAJ |
| description | Telomeres, the protective DNA-protein complexes at the ends of linear chromosomes, are important for genome stability. Leukocyte or peripheral blood mononuclear cell (PBMC) telomere length is a potential biomarker for human aging that integrates genetic, environmental, and lifestyle factors and is associated with mortality and risks for major diseases. However, only a limited number of studies have examined longitudinal changes of telomere length and few have reported data on sorted circulating immune cells. We examined the average telomere length (TL) in CD4+, CD8+CD28+, and CD8+CD28− T cells, B cells, and PBMCs, cross-sectionally and longitudinally, in a cohort of premenopausal women. We report that TL changes over 18 months were correlated among these three T cell types within the same participant. Additionally, PBMC TL change was also correlated with those of all three T cell types, and B cells. The rate of shortening for B cells was significantly greater than for the three T cell types. CD8+CD28− cells, despite having the shortest TL, showed significantly more rapid attrition when compared to CD8+CD28+ T cells. These results suggest systematically coordinated, yet cell type-specific responses to factors and pathways contribute to telomere length regulation. |
| format | Article |
| id | doaj-art-48a1f1ef7877424287eef9edd47c545b |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-48a1f1ef7877424287eef9edd47c545b2025-02-03T01:03:28ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/53710505371050Systematic and Cell Type-Specific Telomere Length Changes in Subsets of LymphocytesJue Lin0Joshua Cheon1Rashida Brown2Michael Coccia3Eli Puterman4Kirstin Aschbacher5Elizabeth Sinclair6Elissa Epel7Elizabeth H. Blackburn8Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USADepartment of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USADivision of Epidemiology, University of California Berkeley, Berkeley, CA 94720, USADepartment of Psychiatry, University of California San Francisco, San Francisco, CA 94118, USASchool of Kinesiology, University of British Columbia, Vancouver, BC, V6T 1Z1, CanadaDepartment of Psychiatry, University of California San Francisco, San Francisco, CA 94118, USACore Immunology Lab, Division of Experimental Medicine, University of California San Francisco, San Francisco, CA 94110, USADepartment of Psychiatry, University of California San Francisco, San Francisco, CA 94118, USADepartment of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USATelomeres, the protective DNA-protein complexes at the ends of linear chromosomes, are important for genome stability. Leukocyte or peripheral blood mononuclear cell (PBMC) telomere length is a potential biomarker for human aging that integrates genetic, environmental, and lifestyle factors and is associated with mortality and risks for major diseases. However, only a limited number of studies have examined longitudinal changes of telomere length and few have reported data on sorted circulating immune cells. We examined the average telomere length (TL) in CD4+, CD8+CD28+, and CD8+CD28− T cells, B cells, and PBMCs, cross-sectionally and longitudinally, in a cohort of premenopausal women. We report that TL changes over 18 months were correlated among these three T cell types within the same participant. Additionally, PBMC TL change was also correlated with those of all three T cell types, and B cells. The rate of shortening for B cells was significantly greater than for the three T cell types. CD8+CD28− cells, despite having the shortest TL, showed significantly more rapid attrition when compared to CD8+CD28+ T cells. These results suggest systematically coordinated, yet cell type-specific responses to factors and pathways contribute to telomere length regulation.http://dx.doi.org/10.1155/2016/5371050 |
| spellingShingle | Jue Lin Joshua Cheon Rashida Brown Michael Coccia Eli Puterman Kirstin Aschbacher Elizabeth Sinclair Elissa Epel Elizabeth H. Blackburn Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes Journal of Immunology Research |
| title | Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes |
| title_full | Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes |
| title_fullStr | Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes |
| title_full_unstemmed | Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes |
| title_short | Systematic and Cell Type-Specific Telomere Length Changes in Subsets of Lymphocytes |
| title_sort | systematic and cell type specific telomere length changes in subsets of lymphocytes |
| url | http://dx.doi.org/10.1155/2016/5371050 |
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