PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver
To investigate the functions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) in the goose fatty liver, a total of 30 healthy 63-day-old male Landes geese were selected and randomly assigned to control group and overfeeding group. The overexpression or RNA interferenc...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011957 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591835461058560 |
|---|---|
| author | Jiahui Li Mengqing Lv Zijin Yuan Jing Ge Tuoyu Geng Daoqing Gong Minmeng Zhao |
| author_facet | Jiahui Li Mengqing Lv Zijin Yuan Jing Ge Tuoyu Geng Daoqing Gong Minmeng Zhao |
| author_sort | Jiahui Li |
| collection | DOAJ |
| description | To investigate the functions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) in the goose fatty liver, a total of 30 healthy 63-day-old male Landes geese were selected and randomly assigned to control group and overfeeding group. The overexpression or RNA interference assay of PGC-1α was performed in goose primary hepatocytes. Our data showed that the PGC-1α expression was increased in fatty liver. The abundance of mitochondrial biosynthesis-related and energy metabolism-related genes, including mitochondrial transcription factor A (TFAM), mitochondrial transcription factor B1 (TFB1M), mitochondrial transcription factor B2 (TFB2M), nuclear respiratory factor 1 (NRF1), DNA topoisomerase I mitochondrial (TOP1MT), peroxisome proliferator-activated receptor gamma coactivator 1-beta (PGC-1β), sirtuin 3 (SIRT3), mitochondrially encoded cytochrome B (CYTB), and AMP-activated protein kinase alpha (AMPKα) were significantly increased in fatty liver. The abundance of TFAM, TFB1M, TFB2M, NRF1, and TOP1MT transcript was induced by PGC-1α overexpression, but inhibited by PGC-1α interference in primary hepatocytes. The mRNA expression levels of PGC-1β, SIRT3, SIRT5, CYTB, and AMPKα were significantly enhanced after PGC-1α overexpression. However, the mRNA expression levels of PGC-1β, SIRT5 and AMPKα were decreased after PGC-1α interference. Furthermore, we observed a significant increase in the mitochondrial DNA (mtDNA) copy number, the activity of mitochondrial respiratory chain complex Ⅳ (MRCC Ⅳ), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), and the NAD+/NADH ratio in fatty liver. But the activity of MRCC Ⅴ, as well as the levels of ADP and ATP in fatty liver were reduced. Additionally, the mtDNA copy number, the activity of MRCC Ⅰ, MRCC Ⅲ-Ⅴ, SDH, and MDH, and NAD+/NADH ratio were enhanced by PGC-1α overexpression; Whereas the mtDNA copy number, the activity of MRCC Ⅰ, SDH, and MDH, and the ratio of NAD+/NADH were inhibited by PGC-1α interference. In conclusion, these findings suggest that PGC-1α improves mitochondrial biosynthesis and energy metabolism in goose fatty liver, which may be an adaptive mechanism for goose fatty liver to cope with steatosis. |
| format | Article |
| id | doaj-art-489aa6160299404dab14083f04cec073 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-489aa6160299404dab14083f04cec0732025-01-22T05:40:44ZengElsevierPoultry Science0032-57912025-01-011041104617PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liverJiahui Li0Mengqing Lv1Zijin Yuan2Jing Ge3Tuoyu Geng4Daoqing Gong5Minmeng Zhao6College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China; Corresponding author: No. 48 Wenhui East Road, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, PR China.To investigate the functions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) in the goose fatty liver, a total of 30 healthy 63-day-old male Landes geese were selected and randomly assigned to control group and overfeeding group. The overexpression or RNA interference assay of PGC-1α was performed in goose primary hepatocytes. Our data showed that the PGC-1α expression was increased in fatty liver. The abundance of mitochondrial biosynthesis-related and energy metabolism-related genes, including mitochondrial transcription factor A (TFAM), mitochondrial transcription factor B1 (TFB1M), mitochondrial transcription factor B2 (TFB2M), nuclear respiratory factor 1 (NRF1), DNA topoisomerase I mitochondrial (TOP1MT), peroxisome proliferator-activated receptor gamma coactivator 1-beta (PGC-1β), sirtuin 3 (SIRT3), mitochondrially encoded cytochrome B (CYTB), and AMP-activated protein kinase alpha (AMPKα) were significantly increased in fatty liver. The abundance of TFAM, TFB1M, TFB2M, NRF1, and TOP1MT transcript was induced by PGC-1α overexpression, but inhibited by PGC-1α interference in primary hepatocytes. The mRNA expression levels of PGC-1β, SIRT3, SIRT5, CYTB, and AMPKα were significantly enhanced after PGC-1α overexpression. However, the mRNA expression levels of PGC-1β, SIRT5 and AMPKα were decreased after PGC-1α interference. Furthermore, we observed a significant increase in the mitochondrial DNA (mtDNA) copy number, the activity of mitochondrial respiratory chain complex Ⅳ (MRCC Ⅳ), succinate dehydrogenase (SDH), malate dehydrogenase (MDH), and the NAD+/NADH ratio in fatty liver. But the activity of MRCC Ⅴ, as well as the levels of ADP and ATP in fatty liver were reduced. Additionally, the mtDNA copy number, the activity of MRCC Ⅰ, MRCC Ⅲ-Ⅴ, SDH, and MDH, and NAD+/NADH ratio were enhanced by PGC-1α overexpression; Whereas the mtDNA copy number, the activity of MRCC Ⅰ, SDH, and MDH, and the ratio of NAD+/NADH were inhibited by PGC-1α interference. In conclusion, these findings suggest that PGC-1α improves mitochondrial biosynthesis and energy metabolism in goose fatty liver, which may be an adaptive mechanism for goose fatty liver to cope with steatosis.http://www.sciencedirect.com/science/article/pii/S0032579124011957PGC-1αGoose fatty liverMitochondriaMitochondrial biosynthesisEnergy metabolism |
| spellingShingle | Jiahui Li Mengqing Lv Zijin Yuan Jing Ge Tuoyu Geng Daoqing Gong Minmeng Zhao PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver Poultry Science PGC-1α Goose fatty liver Mitochondria Mitochondrial biosynthesis Energy metabolism |
| title | PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver |
| title_full | PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver |
| title_fullStr | PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver |
| title_full_unstemmed | PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver |
| title_short | PGC-1α Promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver |
| title_sort | pgc 1α promotes mitochondrial biosynthesis and energy metabolism of goose fatty liver |
| topic | PGC-1α Goose fatty liver Mitochondria Mitochondrial biosynthesis Energy metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011957 |
| work_keys_str_mv | AT jiahuili pgc1apromotesmitochondrialbiosynthesisandenergymetabolismofgoosefattyliver AT mengqinglv pgc1apromotesmitochondrialbiosynthesisandenergymetabolismofgoosefattyliver AT zijinyuan pgc1apromotesmitochondrialbiosynthesisandenergymetabolismofgoosefattyliver AT jingge pgc1apromotesmitochondrialbiosynthesisandenergymetabolismofgoosefattyliver AT tuoyugeng pgc1apromotesmitochondrialbiosynthesisandenergymetabolismofgoosefattyliver AT daoqinggong pgc1apromotesmitochondrialbiosynthesisandenergymetabolismofgoosefattyliver AT minmengzhao pgc1apromotesmitochondrialbiosynthesisandenergymetabolismofgoosefattyliver |