Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour Tissue
Background: The simultaneous detection of protein expression and gene copy number changes in patient samples, like paraffin-embedded tissue sections, is challenging since the procedures of immunohistochemistry (IHC) and Fluorescence in situ Hybridization (FISH) negatively influence each other which...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.3233/ACP-CLO-2010-0532 |
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| _version_ | 1832555043438460928 |
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| author | Timo Gaiser Lissa Berroa-Garcia Ralf Kemmerling Aparajita Dutta Thomas Ried Kerstin Heselmeyer-Haddad |
| author_facet | Timo Gaiser Lissa Berroa-Garcia Ralf Kemmerling Aparajita Dutta Thomas Ried Kerstin Heselmeyer-Haddad |
| author_sort | Timo Gaiser |
| collection | DOAJ |
| description | Background: The simultaneous detection of protein expression and gene copy number changes in patient samples, like paraffin-embedded tissue sections, is challenging since the procedures of immunohistochemistry (IHC) and Fluorescence in situ Hybridization (FISH) negatively influence each other which often results in suboptimal staining. Therefore, we developed a novel automated algorithm based on relocation which allows subsequent detection of protein content and gene copy number changes within the same cell. |
| format | Article |
| id | doaj-art-4893dca127924e17901960d911d2bab2 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-4893dca127924e17901960d911d2bab22025-02-03T05:49:47ZengWileyAnalytical Cellular Pathology2210-71772210-71852010-01-0133210511210.3233/ACP-CLO-2010-0532Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour TissueTimo Gaiser0Lissa Berroa-Garcia1Ralf Kemmerling2Aparajita Dutta3Thomas Ried4Kerstin Heselmeyer-Haddad5Section of Cancer Genomics, National Cancer Institute, National Institutes of Health, Bethesda, MD, USASection of Cancer Genomics, National Cancer Institute, National Institutes of Health, Bethesda, MD, USAInstitute for Pathology, Paracelsus Medical University, Salzburg, AustriaMetaSystems, Waltham, MA, USASection of Cancer Genomics, National Cancer Institute, National Institutes of Health, Bethesda, MD, USASection of Cancer Genomics, National Cancer Institute, National Institutes of Health, Bethesda, MD, USABackground: The simultaneous detection of protein expression and gene copy number changes in patient samples, like paraffin-embedded tissue sections, is challenging since the procedures of immunohistochemistry (IHC) and Fluorescence in situ Hybridization (FISH) negatively influence each other which often results in suboptimal staining. Therefore, we developed a novel automated algorithm based on relocation which allows subsequent detection of protein content and gene copy number changes within the same cell.http://dx.doi.org/10.3233/ACP-CLO-2010-0532 |
| spellingShingle | Timo Gaiser Lissa Berroa-Garcia Ralf Kemmerling Aparajita Dutta Thomas Ried Kerstin Heselmeyer-Haddad Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour Tissue Analytical Cellular Pathology |
| title | Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour Tissue |
| title_full | Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour Tissue |
| title_fullStr | Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour Tissue |
| title_full_unstemmed | Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour Tissue |
| title_short | Automated Analysis of Protein Expression and Gene Amplification within the Same Cells of Paraffin-Embedded Tumour Tissue |
| title_sort | automated analysis of protein expression and gene amplification within the same cells of paraffin embedded tumour tissue |
| url | http://dx.doi.org/10.3233/ACP-CLO-2010-0532 |
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