Complete Genome Sequence Analysis of Bacillus subtilis MC4-2 Strain That against Tobacco Black Shank Disease
The MC4-2 bacterium strain was isolated and purified from the Periplaneta americana intestine as a biocontrol agent with good antagonistic effect against the pathogens of a soil-borne disease called tobacco black shank. The MC4-2 strain was found to have good broad-spectrum inhibition by plate stand...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2024/8846747 |
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| author | Chunlan Shi Shuquan Zeng Xi Gao Mehboob Hussain Mingchuan He Xurong Niu Congcong Wei Rui Yang Mingxian Lan Yonghui Xie Zhijiang Wang Guoxing Wu Ping Tang |
| author_facet | Chunlan Shi Shuquan Zeng Xi Gao Mehboob Hussain Mingchuan He Xurong Niu Congcong Wei Rui Yang Mingxian Lan Yonghui Xie Zhijiang Wang Guoxing Wu Ping Tang |
| author_sort | Chunlan Shi |
| collection | DOAJ |
| description | The MC4-2 bacterium strain was isolated and purified from the Periplaneta americana intestine as a biocontrol agent with good antagonistic effect against the pathogens of a soil-borne disease called tobacco black shank. The MC4-2 strain was found to have good broad-spectrum inhibition by plate stand-off test. Based on 16S rRNA and gyrB genes, ANI analysis, and other comparative genomics methods, it was determined that the MC4-2 strain was Bacillus subtilis. The complete genome sequence showed that the genome size was 4,076,630 bp, the average GC content was 43.78%, and the total number of CDSs was 4,207. Genomic prediction analysis revealed that a total of 145 genes were annotated by the CAZy, containing mainly GH and CE enzymes that break down carbohydrates such as glucose, chitin, starch, and alginate, and a large number of enzymes involved in glycosylation were present. A total of ten secondary metabolite clusters were predicted, six clusters of which were annotated as surfactin, bacillaene, fengycin, bacillibactin, subtilosin A, and bacilysin. The present investigation found the biological control mechanism of B. subtilis MC4-2, which provides a strong theoretical basis for the best use of this strain in biological control methods and provides a reference for the subsequent development of agents of this bacterium. |
| format | Article |
| id | doaj-art-4889823f21854d5e8605569d1b611135 |
| institution | Kabale University |
| issn | 2314-4378 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-4889823f21854d5e8605569d1b6111352025-02-03T06:14:53ZengWileyInternational Journal of Genomics2314-43782024-01-01202410.1155/2024/8846747Complete Genome Sequence Analysis of Bacillus subtilis MC4-2 Strain That against Tobacco Black Shank DiseaseChunlan Shi0Shuquan Zeng1Xi Gao2Mehboob Hussain3Mingchuan He4Xurong Niu5Congcong Wei6Rui Yang7Mingxian Lan8Yonghui Xie9Zhijiang Wang10Guoxing Wu11Ping Tang12State Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanYunnan Tobacco Company Kunming CompanyYunnan Tobacco Company Kunming CompanyState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanState Key Laboratory for Conservation and Utilization of Bio-Resources in YunnanThe MC4-2 bacterium strain was isolated and purified from the Periplaneta americana intestine as a biocontrol agent with good antagonistic effect against the pathogens of a soil-borne disease called tobacco black shank. The MC4-2 strain was found to have good broad-spectrum inhibition by plate stand-off test. Based on 16S rRNA and gyrB genes, ANI analysis, and other comparative genomics methods, it was determined that the MC4-2 strain was Bacillus subtilis. The complete genome sequence showed that the genome size was 4,076,630 bp, the average GC content was 43.78%, and the total number of CDSs was 4,207. Genomic prediction analysis revealed that a total of 145 genes were annotated by the CAZy, containing mainly GH and CE enzymes that break down carbohydrates such as glucose, chitin, starch, and alginate, and a large number of enzymes involved in glycosylation were present. A total of ten secondary metabolite clusters were predicted, six clusters of which were annotated as surfactin, bacillaene, fengycin, bacillibactin, subtilosin A, and bacilysin. The present investigation found the biological control mechanism of B. subtilis MC4-2, which provides a strong theoretical basis for the best use of this strain in biological control methods and provides a reference for the subsequent development of agents of this bacterium.http://dx.doi.org/10.1155/2024/8846747 |
| spellingShingle | Chunlan Shi Shuquan Zeng Xi Gao Mehboob Hussain Mingchuan He Xurong Niu Congcong Wei Rui Yang Mingxian Lan Yonghui Xie Zhijiang Wang Guoxing Wu Ping Tang Complete Genome Sequence Analysis of Bacillus subtilis MC4-2 Strain That against Tobacco Black Shank Disease International Journal of Genomics |
| title | Complete Genome Sequence Analysis of Bacillus subtilis MC4-2 Strain That against Tobacco Black Shank Disease |
| title_full | Complete Genome Sequence Analysis of Bacillus subtilis MC4-2 Strain That against Tobacco Black Shank Disease |
| title_fullStr | Complete Genome Sequence Analysis of Bacillus subtilis MC4-2 Strain That against Tobacco Black Shank Disease |
| title_full_unstemmed | Complete Genome Sequence Analysis of Bacillus subtilis MC4-2 Strain That against Tobacco Black Shank Disease |
| title_short | Complete Genome Sequence Analysis of Bacillus subtilis MC4-2 Strain That against Tobacco Black Shank Disease |
| title_sort | complete genome sequence analysis of bacillus subtilis mc4 2 strain that against tobacco black shank disease |
| url | http://dx.doi.org/10.1155/2024/8846747 |
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