Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral Leishmaniasis
We performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB). After twelve months, the results obtained from IFA demonstrated that 50% were serop...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2009/680790 |
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| author | Alba Valéria Machado da Silva Adelzon Assis de Paula Daniela de Pita Pereira Reginaldo Peçanha Brazil João Carlos Araujo Carreira |
| author_facet | Alba Valéria Machado da Silva Adelzon Assis de Paula Daniela de Pita Pereira Reginaldo Peçanha Brazil João Carlos Araujo Carreira |
| author_sort | Alba Valéria Machado da Silva |
| collection | DOAJ |
| description | We performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB). After twelve months, the results obtained from IFA demonstrated that 50% were seropositive and two serological profiles were observed: the first one ranging from 1/40 to 1/80 and the second ≥1/160. By WB, it was observed that the same percentage and sera from positive dogs presented the recognition of the peptides of 29 and 32 kDa up to 8 months before IFA serum conversion. Among the positive dogs, all the sera from symptomatic ones with tissue parasitism recognized the peptide of 68.5 kDa. Our results suggest the need of modifications in the control measures regarding the elimination of the dogs. They also corroborate the high sensitivity and specificity of western blot in the diagnosis of canine leishmaniasis, suggesting the possibility of its association with IFA. |
| format | Article |
| id | doaj-art-487f9dffb43b490e9b7ffd062d552d19 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-487f9dffb43b490e9b7ffd062d552d192025-02-03T01:02:45ZengWileyJournal of Parasitology Research2090-00232090-00312009-01-01200910.1155/2009/680790680790Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral LeishmaniasisAlba Valéria Machado da Silva0Adelzon Assis de Paula1Daniela de Pita Pereira2Reginaldo Peçanha Brazil3João Carlos Araujo Carreira4Departamento de Protozoologia, FIOCRUZ, Rio de Janeiro, CEP 21040-360, BrazilCentro de Ciências de Saúde, UFRJ, Rio de Janeiro, CEP 21941-901, BrazilLaboratório de Bioquímica Molecular de Doenças Endêmicas, FIOCRUZ, Rio de Janeiro, CEP 21040-360, BrazilLaboratório de Bioquímica e Fisiologia de Insetos, FIOCRUZ, Rio de Janeiro, CEP 21040-360, BrazilDepartamento de Protozoologia, FIOCRUZ, Rio de Janeiro, CEP 21040-360, BrazilWe performed a serological, clinical, and parasitological follow-up of a dog population in an endemic area of American Visceral Leishmaniasis estimated by indirect immunofluorescent assay (IFA) and western blot (WB). After twelve months, the results obtained from IFA demonstrated that 50% were seropositive and two serological profiles were observed: the first one ranging from 1/40 to 1/80 and the second ≥1/160. By WB, it was observed that the same percentage and sera from positive dogs presented the recognition of the peptides of 29 and 32 kDa up to 8 months before IFA serum conversion. Among the positive dogs, all the sera from symptomatic ones with tissue parasitism recognized the peptide of 68.5 kDa. Our results suggest the need of modifications in the control measures regarding the elimination of the dogs. They also corroborate the high sensitivity and specificity of western blot in the diagnosis of canine leishmaniasis, suggesting the possibility of its association with IFA.http://dx.doi.org/10.1155/2009/680790 |
| spellingShingle | Alba Valéria Machado da Silva Adelzon Assis de Paula Daniela de Pita Pereira Reginaldo Peçanha Brazil João Carlos Araujo Carreira Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral Leishmaniasis Journal of Parasitology Research |
| title | Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral Leishmaniasis |
| title_full | Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral Leishmaniasis |
| title_fullStr | Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral Leishmaniasis |
| title_full_unstemmed | Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral Leishmaniasis |
| title_short | Canine Leishmaniasis in Brazil: Serological Follow-Up of a Dog Population in an Endemic Area of American Visceral Leishmaniasis |
| title_sort | canine leishmaniasis in brazil serological follow up of a dog population in an endemic area of american visceral leishmaniasis |
| url | http://dx.doi.org/10.1155/2009/680790 |
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