A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2
Silicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate (TPP) cross-linked networks. In this study, heparin was covalently bonded t...
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| Format: | Article |
| Language: | English |
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Sciendo
2016-09-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2016-0026 |
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| author | Thanyaphoo Suphannee Kaewsrichan Jasadee |
| author_facet | Thanyaphoo Suphannee Kaewsrichan Jasadee |
| author_sort | Thanyaphoo Suphannee |
| collection | DOAJ |
| description | Silicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate (TPP) cross-linked networks. In this study, heparin was covalently bonded to the residual -NH2 groups of chitosan on the scaffold applying carbodiimide chemistry. Bonded heparin was not leached away from scaffold surfaces upon vigorous washing or extended storage. Recombinant human bone morphogenetic protein 2 (rhBMP-2) was bound to conjugated scaffolds by ionic interactions between the negatively charged SO42- clusters of heparin and positively charged amino acids of rhBMP-2. The resulting scaffolds were inspected for bone regenerative capacity by subcutaneous implanting in rats. Histological observation and mineralization assay were performed after 4 weeks of implantation. Results from both in vitro and in vivo experiments suggest the potential of the developed scaffolds for bone tissue engineering applications in the future. |
| format | Article |
| id | doaj-art-487a7521099d49c2b1523a0141bf0591 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-487a7521099d49c2b1523a0141bf05912025-02-02T09:57:48ZengSciendoActa Pharmaceutica1846-95582016-09-0166337338510.1515/acph-2016-0026acph-2016-0026A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2Thanyaphoo Suphannee0Kaewsrichan Jasadee1Department of Pharmaceutical Chemistry Faculty of Pharmaceutical Sciences Prince of Songkla University Hat-yai, Songkhla, ThailandDepartment of Pharmaceutical Chemistry Faculty of Pharmaceutical Sciences and Nanotec-PSU Center of Excellence on Drug Delivery System Prince of Songkla University Hat-yai, Songkhla, ThailandSilicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate (TPP) cross-linked networks. In this study, heparin was covalently bonded to the residual -NH2 groups of chitosan on the scaffold applying carbodiimide chemistry. Bonded heparin was not leached away from scaffold surfaces upon vigorous washing or extended storage. Recombinant human bone morphogenetic protein 2 (rhBMP-2) was bound to conjugated scaffolds by ionic interactions between the negatively charged SO42- clusters of heparin and positively charged amino acids of rhBMP-2. The resulting scaffolds were inspected for bone regenerative capacity by subcutaneous implanting in rats. Histological observation and mineralization assay were performed after 4 weeks of implantation. Results from both in vitro and in vivo experiments suggest the potential of the developed scaffolds for bone tissue engineering applications in the future.https://doi.org/10.1515/acph-2016-0026heparincarbodiimiderhbmp-2delivery scaffoldbone tissue engineering |
| spellingShingle | Thanyaphoo Suphannee Kaewsrichan Jasadee A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2 Acta Pharmaceutica heparin carbodiimide rhbmp-2 delivery scaffold bone tissue engineering |
| title | A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2 |
| title_full | A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2 |
| title_fullStr | A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2 |
| title_full_unstemmed | A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2 |
| title_short | A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2 |
| title_sort | new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2 |
| topic | heparin carbodiimide rhbmp-2 delivery scaffold bone tissue engineering |
| url | https://doi.org/10.1515/acph-2016-0026 |
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