Growth factors IGF-1 and KGF and adipose-derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound model
IntroductionIn the field of plastic surgery, epidermal transplantation is a potential treatment for chronic wounds that results in only minor donor site morbidity. Improving the regenerative capacities of epidermal grafts or single-cell suspensions and therefore accelerating healing processes would...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1516116/full |
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| author | Nina Stadelmann Raymund E. Horch Rafael Schmid David Ostendorf Ajay Peddi Theresa Promny Anja M. Boos Annika Kengelbach-Weigand |
| author_facet | Nina Stadelmann Raymund E. Horch Rafael Schmid David Ostendorf Ajay Peddi Theresa Promny Anja M. Boos Annika Kengelbach-Weigand |
| author_sort | Nina Stadelmann |
| collection | DOAJ |
| description | IntroductionIn the field of plastic surgery, epidermal transplantation is a potential treatment for chronic wounds that results in only minor donor site morbidity. Improving the regenerative capacities of epidermal grafts or single-cell suspensions and therefore accelerating healing processes would be of significant interest.MethodsIn the present study, we analyzed the effects of growth factors and adipose-derived stem cells (ADSCs) on keratinocyte properties. For optimum translation into the clinical setting, primary human keratinocytes and patient-matched ADSCs were isolated and used in an in vitro wound model.ResultsThe keratinocyte migration and viability increased after treatment with the growth factors insulin-like growth factor 1 (IGF-1) and keratinocyte growth factor (KGF). A similar effect was observed with the use of a concentrated ADSC-conditioned medium (ADSC-CM). It was further possible to isolate the keratinocytes in a xenogen-free medium, which is essential for clinical translation. Importantly, a patient-dependent influence on the effects of the growth factors and ADSC-CM was observed.DiscussionThis study provides potential for the improvement of epidermal transplantation in the treatment of chronic wounds using xenogen-free isolated and cultivated keratinocytes, growth factors, and ADSC. Translating these results into clinical application may help accelerate wound healing and shorten the time until patients can return to everyday life. |
| format | Article |
| id | doaj-art-486392a1522645f7adb0e5846af24a23 |
| institution | Kabale University |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj-art-486392a1522645f7adb0e5846af24a232025-02-06T07:10:21ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-02-011210.3389/fmed.2025.15161161516116Growth factors IGF-1 and KGF and adipose-derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound modelNina StadelmannRaymund E. HorchRafael SchmidDavid OstendorfAjay PeddiTheresa PromnyAnja M. BoosAnnika Kengelbach-WeigandIntroductionIn the field of plastic surgery, epidermal transplantation is a potential treatment for chronic wounds that results in only minor donor site morbidity. Improving the regenerative capacities of epidermal grafts or single-cell suspensions and therefore accelerating healing processes would be of significant interest.MethodsIn the present study, we analyzed the effects of growth factors and adipose-derived stem cells (ADSCs) on keratinocyte properties. For optimum translation into the clinical setting, primary human keratinocytes and patient-matched ADSCs were isolated and used in an in vitro wound model.ResultsThe keratinocyte migration and viability increased after treatment with the growth factors insulin-like growth factor 1 (IGF-1) and keratinocyte growth factor (KGF). A similar effect was observed with the use of a concentrated ADSC-conditioned medium (ADSC-CM). It was further possible to isolate the keratinocytes in a xenogen-free medium, which is essential for clinical translation. Importantly, a patient-dependent influence on the effects of the growth factors and ADSC-CM was observed.DiscussionThis study provides potential for the improvement of epidermal transplantation in the treatment of chronic wounds using xenogen-free isolated and cultivated keratinocytes, growth factors, and ADSC. Translating these results into clinical application may help accelerate wound healing and shorten the time until patients can return to everyday life.https://www.frontiersin.org/articles/10.3389/fmed.2025.1516116/fullkeratinocyteswound healinggrowth factorsadipose-derived stem cellsconditioned medium |
| spellingShingle | Nina Stadelmann Raymund E. Horch Rafael Schmid David Ostendorf Ajay Peddi Theresa Promny Anja M. Boos Annika Kengelbach-Weigand Growth factors IGF-1 and KGF and adipose-derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound model Frontiers in Medicine keratinocytes wound healing growth factors adipose-derived stem cells conditioned medium |
| title | Growth factors IGF-1 and KGF and adipose-derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound model |
| title_full | Growth factors IGF-1 and KGF and adipose-derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound model |
| title_fullStr | Growth factors IGF-1 and KGF and adipose-derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound model |
| title_full_unstemmed | Growth factors IGF-1 and KGF and adipose-derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound model |
| title_short | Growth factors IGF-1 and KGF and adipose-derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound model |
| title_sort | growth factors igf 1 and kgf and adipose derived stem cells promote migration and viability of primary human keratinocytes in an in vitro wound model |
| topic | keratinocytes wound healing growth factors adipose-derived stem cells conditioned medium |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1516116/full |
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