Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA
A zinc finger motif is an element of proteins that can specifically recognize and bind to DNA. Because they contain multiple cysteine residues, zinc finger motifs possess redox properties. Ionizing radiation generates a variety of free radicals in organisms. Zinc finger motifs, therefore, may be a t...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2016/1642064 |
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| author | XiaoHong Zhang YuJi Miao XiaoDan Hu Rui Min PeiDang Liu HaiQian Zhang |
| author_facet | XiaoHong Zhang YuJi Miao XiaoDan Hu Rui Min PeiDang Liu HaiQian Zhang |
| author_sort | XiaoHong Zhang |
| collection | DOAJ |
| description | A zinc finger motif is an element of proteins that can specifically recognize and bind to DNA. Because they contain multiple cysteine residues, zinc finger motifs possess redox properties. Ionizing radiation generates a variety of free radicals in organisms. Zinc finger motifs, therefore, may be a target of ionizing radiation. The effect of gamma radiation on the zinc finger motifs in transcription factor IIIA (TFIIIA), a zinc finger protein, was investigated. TFIIIA was exposed to different gamma doses from 60Co sources. The dose rates were 0.20 Gy/min and 800 Gy/h, respectively. The binding capacity of zinc finger motifs in TFIIIA was determined using an electrophoretic mobility shift assay. We found that 1000 Gy of gamma radiation impaired the function of the zinc finger motifs in TFIIIA. The sites of radiation-induced damage in the zinc finger were the thiol groups of cysteine residues and zinc (II) ions. The thiol groups were oxidized to form disulfide bonds and the zinc (II) ions were indicated to be reduced to zinc atoms. These results indicate that the zinc finger motif is a target domain for gamma radiation, which may decrease 5S rRNA expression via impairment of the zinc finger motifs in TFIIIA. |
| format | Article |
| id | doaj-art-483b60bd35af4ca4b46dca45114f7248 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-483b60bd35af4ca4b46dca45114f72482025-02-03T05:45:24ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2016-01-01201610.1155/2016/16420641642064Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIAXiaoHong Zhang0YuJi Miao1XiaoDan Hu2Rui Min3PeiDang Liu4HaiQian Zhang5Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, ChinaDepartment of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, ChinaDepartment of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, ChinaDivision of Radiation Medicine, Department of Naval Medicine, Second Military Medical University, Shanghai 200433, ChinaJiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing 210009, ChinaDepartment of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, ChinaA zinc finger motif is an element of proteins that can specifically recognize and bind to DNA. Because they contain multiple cysteine residues, zinc finger motifs possess redox properties. Ionizing radiation generates a variety of free radicals in organisms. Zinc finger motifs, therefore, may be a target of ionizing radiation. The effect of gamma radiation on the zinc finger motifs in transcription factor IIIA (TFIIIA), a zinc finger protein, was investigated. TFIIIA was exposed to different gamma doses from 60Co sources. The dose rates were 0.20 Gy/min and 800 Gy/h, respectively. The binding capacity of zinc finger motifs in TFIIIA was determined using an electrophoretic mobility shift assay. We found that 1000 Gy of gamma radiation impaired the function of the zinc finger motifs in TFIIIA. The sites of radiation-induced damage in the zinc finger were the thiol groups of cysteine residues and zinc (II) ions. The thiol groups were oxidized to form disulfide bonds and the zinc (II) ions were indicated to be reduced to zinc atoms. These results indicate that the zinc finger motif is a target domain for gamma radiation, which may decrease 5S rRNA expression via impairment of the zinc finger motifs in TFIIIA.http://dx.doi.org/10.1155/2016/1642064 |
| spellingShingle | XiaoHong Zhang YuJi Miao XiaoDan Hu Rui Min PeiDang Liu HaiQian Zhang Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA Bioinorganic Chemistry and Applications |
| title | Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA |
| title_full | Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA |
| title_fullStr | Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA |
| title_full_unstemmed | Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA |
| title_short | Gamma Radiation-Induced Damage in the Zinc Finger of the Transcription Factor IIIA |
| title_sort | gamma radiation induced damage in the zinc finger of the transcription factor iiia |
| url | http://dx.doi.org/10.1155/2016/1642064 |
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