Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages
During our ongoing screening program designed to determine the anti-inflammatory potential of natural compounds, we isolated sargachromenol from Sargassum micracanthum. In the present study, we investigated the anti-inflammatory effects of sargachromenol on lipopolysaccharide (LPS)-induced inflammat...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/712303 |
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| author | Eun-Jin Yang Young Min Ham Kyong-Wol Yang Nam Ho Lee Chang-Gu Hyun |
| author_facet | Eun-Jin Yang Young Min Ham Kyong-Wol Yang Nam Ho Lee Chang-Gu Hyun |
| author_sort | Eun-Jin Yang |
| collection | DOAJ |
| description | During our ongoing screening program designed to determine the anti-inflammatory potential of natural compounds, we isolated sargachromenol from Sargassum micracanthum. In the present study, we investigated the anti-inflammatory effects of sargachromenol on lipopolysaccharide (LPS)-induced inflammation in murine RAW 264.7 macrophage cells and the underlying mechanisms. Sargachromenol significantly inhibited the LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in a dose-dependent manner. It also significantly inhibited the protein expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner in LPS-stimulated macrophage cells. Further analyses showed that sargachromenol decreased the cytoplasmic loss of inhibitor κBα (IκBα) protein. These results suggest that sargachromenol may exert its anti-inflammatory effects on LPS-stimulated macrophage cells by inhibiting the activation of the NF-κB signaling pathway. In conclusion, to our knowledge, this is the first study to show that sargachromenol isolated from S. micracanthum has an effective anti-inflammatory activity. Therefore, sargachromenol might be useful for cosmetic, food, or medical applications requiring anti-inflammatory properties. |
| format | Article |
| id | doaj-art-48332e09b31e45e0a2b7ab30190189d9 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-48332e09b31e45e0a2b7ab30190189d92025-02-03T01:12:49ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/712303712303Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 MacrophagesEun-Jin Yang0Young Min Ham1Kyong-Wol Yang2Nam Ho Lee3Chang-Gu Hyun4Jeju Biodiversity Research Institute (JBRI), Jeju Technopark, Jeju 699-943, Republic of KoreaJeju Biodiversity Research Institute (JBRI), Jeju Technopark, Jeju 699-943, Republic of KoreaJeju Love Co., Ltd., 542-5 Haengwon-ri, Gujwa-eup, Jeju 695-975, Republic of KoreaCosmetic Science Center, Department of Chemistry, Jeju National University, Jeju 690-756, Republic of KoreaCosmetic Science Center, Department of Chemistry, Jeju National University, Jeju 690-756, Republic of KoreaDuring our ongoing screening program designed to determine the anti-inflammatory potential of natural compounds, we isolated sargachromenol from Sargassum micracanthum. In the present study, we investigated the anti-inflammatory effects of sargachromenol on lipopolysaccharide (LPS)-induced inflammation in murine RAW 264.7 macrophage cells and the underlying mechanisms. Sargachromenol significantly inhibited the LPS-induced production of nitric oxide (NO) and prostaglandin E2 (PGE2) in a dose-dependent manner. It also significantly inhibited the protein expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner in LPS-stimulated macrophage cells. Further analyses showed that sargachromenol decreased the cytoplasmic loss of inhibitor κBα (IκBα) protein. These results suggest that sargachromenol may exert its anti-inflammatory effects on LPS-stimulated macrophage cells by inhibiting the activation of the NF-κB signaling pathway. In conclusion, to our knowledge, this is the first study to show that sargachromenol isolated from S. micracanthum has an effective anti-inflammatory activity. Therefore, sargachromenol might be useful for cosmetic, food, or medical applications requiring anti-inflammatory properties.http://dx.doi.org/10.1155/2013/712303 |
| spellingShingle | Eun-Jin Yang Young Min Ham Kyong-Wol Yang Nam Ho Lee Chang-Gu Hyun Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages The Scientific World Journal |
| title | Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages |
| title_full | Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages |
| title_fullStr | Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages |
| title_full_unstemmed | Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages |
| title_short | Sargachromenol from Sargassum micracanthum Inhibits the Lipopolysaccharide-Induced Production of Inflammatory Mediators in RAW 264.7 Macrophages |
| title_sort | sargachromenol from sargassum micracanthum inhibits the lipopolysaccharide induced production of inflammatory mediators in raw 264 7 macrophages |
| url | http://dx.doi.org/10.1155/2013/712303 |
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