Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis
Human amniotic fluid stem cells have become an attractive stem cell source for potential applications in regenerative medicine and tissue engineering. The aim of this study was to characterize amniotic fluid-derived mesenchymal stem cells (AF-MSCs) from second- and third-trimester of gestation. Usin...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/319238 |
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| author | Jurate Savickiene Grazina Treigyte Sandra Baronaite Giedre Valiuliene Algirdas Kaupinis Mindaugas Valius Audrone Arlauskiene Ruta Navakauskiene |
| author_facet | Jurate Savickiene Grazina Treigyte Sandra Baronaite Giedre Valiuliene Algirdas Kaupinis Mindaugas Valius Audrone Arlauskiene Ruta Navakauskiene |
| author_sort | Jurate Savickiene |
| collection | DOAJ |
| description | Human amniotic fluid stem cells have become an attractive stem cell source for potential applications in regenerative medicine and tissue engineering. The aim of this study was to characterize amniotic fluid-derived mesenchymal stem cells (AF-MSCs) from second- and third-trimester of gestation. Using two-stage protocol, MSCs were successfully cultured and exhibited typical stem cell morphological, specific cell surface, and pluripotency markers characteristics. AF-MSCs differentiated into adipocytes, osteocytes, chondrocytes, myocytes, and neuronal cells, as determined by morphological changes, cell staining, and RT-qPCR showing the tissue-specific gene presence for differentiated cell lineages. Using SYNAPT G2 High Definition Mass Spectrometry technique approach, we performed for the first time the comparative proteomic analysis between undifferentiated AF-MSCs from late trimester of gestation and differentiated into myogenic, adipogenic, osteogenic, and neurogenic lineages. The analysis of the functional and expression patterns of 250 high abundance proteins selected from more than 1400 demonstrated the similar proteome of cultured and differentiated AF-MSCs but the unique changes in their expression profile during cell differentiation that may help the identification of key markers in differentiated cells. Our results provide evidence that human amniotic fluid of second- and third-trimester contains stem cells with multilineage potential and may be attractive source for clinical applications. |
| format | Article |
| id | doaj-art-481537ba80724eb3ba0e0eeaacee246f |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-481537ba80724eb3ba0e0eeaacee246f2025-02-03T06:11:10ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/319238319238Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome AnalysisJurate Savickiene0Grazina Treigyte1Sandra Baronaite2Giedre Valiuliene3Algirdas Kaupinis4Mindaugas Valius5Audrone Arlauskiene6Ruta Navakauskiene7Department of Molecular Cell Biology, Vilnius University, LT-08662 Vilnius, LithuaniaDepartment of Molecular Cell Biology, Vilnius University, LT-08662 Vilnius, LithuaniaDepartment of Molecular Cell Biology, Vilnius University, LT-08662 Vilnius, LithuaniaDepartment of Molecular Cell Biology, Vilnius University, LT-08662 Vilnius, LithuaniaProteomic Center, Institute of Biochemistry, Vilnius University, LT-08662 Vilnius, LithuaniaProteomic Center, Institute of Biochemistry, Vilnius University, LT-08662 Vilnius, LithuaniaClinics of Obstetrics and Gynecology, Faculty of Medicine, Vilnius University, M. K. Čiurlionio Gatvė 21, LT-03101 Vilnius, LithuaniaDepartment of Molecular Cell Biology, Vilnius University, LT-08662 Vilnius, LithuaniaHuman amniotic fluid stem cells have become an attractive stem cell source for potential applications in regenerative medicine and tissue engineering. The aim of this study was to characterize amniotic fluid-derived mesenchymal stem cells (AF-MSCs) from second- and third-trimester of gestation. Using two-stage protocol, MSCs were successfully cultured and exhibited typical stem cell morphological, specific cell surface, and pluripotency markers characteristics. AF-MSCs differentiated into adipocytes, osteocytes, chondrocytes, myocytes, and neuronal cells, as determined by morphological changes, cell staining, and RT-qPCR showing the tissue-specific gene presence for differentiated cell lineages. Using SYNAPT G2 High Definition Mass Spectrometry technique approach, we performed for the first time the comparative proteomic analysis between undifferentiated AF-MSCs from late trimester of gestation and differentiated into myogenic, adipogenic, osteogenic, and neurogenic lineages. The analysis of the functional and expression patterns of 250 high abundance proteins selected from more than 1400 demonstrated the similar proteome of cultured and differentiated AF-MSCs but the unique changes in their expression profile during cell differentiation that may help the identification of key markers in differentiated cells. Our results provide evidence that human amniotic fluid of second- and third-trimester contains stem cells with multilineage potential and may be attractive source for clinical applications.http://dx.doi.org/10.1155/2015/319238 |
| spellingShingle | Jurate Savickiene Grazina Treigyte Sandra Baronaite Giedre Valiuliene Algirdas Kaupinis Mindaugas Valius Audrone Arlauskiene Ruta Navakauskiene Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis Stem Cells International |
| title | Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis |
| title_full | Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis |
| title_fullStr | Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis |
| title_full_unstemmed | Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis |
| title_short | Human Amniotic Fluid Mesenchymal Stem Cells from Second- and Third-Trimester Amniocentesis: Differentiation Potential, Molecular Signature, and Proteome Analysis |
| title_sort | human amniotic fluid mesenchymal stem cells from second and third trimester amniocentesis differentiation potential molecular signature and proteome analysis |
| url | http://dx.doi.org/10.1155/2015/319238 |
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