Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain
The recruitment and activation of regulatory T cells (Tregs) in the micro-environment of malignant brain tumors has detrimental effects on antitumoral immune responses. Hence, local elimination of Tregs within the tumor micro-environment represents a highly valuable tool from both a fundamental and...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/952469 |
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| author | Wim Maes Tina Verschuere Anaïs Van Hoylandt Louis Boon Stefaan Van Gool |
| author_facet | Wim Maes Tina Verschuere Anaïs Van Hoylandt Louis Boon Stefaan Van Gool |
| author_sort | Wim Maes |
| collection | DOAJ |
| description | The recruitment and activation of regulatory T cells (Tregs) in the micro-environment of malignant brain tumors has detrimental effects on antitumoral immune responses. Hence, local elimination of Tregs within the tumor micro-environment represents a highly valuable tool from both a fundamental and clinical perspective. In the syngeneic experimental GL261 murine glioma model, Tregs were prophylactically eliminated through treatment with PC61, an anti-CD25 mAb. This resulted in specific elimination of CD4+CD25hiFoxp3+ Treg within brain-infiltrating lymphocytes and complete protection against subsequent orthotopic GL261 tumor challenge. Interestingly, PC61-treated mice also showed a pronounced infiltration of CD11b+ myeloid cells in the brain. Phenotypically, these cells could not be considered as Gr-1+ myeloid-derived suppressor cells (MDSC) but were identified as F4/80+ macrophages and granulocytes. |
| format | Article |
| id | doaj-art-47fbe53a94f84bb48565df0d76eab30e |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-47fbe53a94f84bb48565df0d76eab30e2025-02-03T06:06:25ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/952469952469Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the BrainWim Maes0Tina Verschuere1Anaïs Van Hoylandt2Louis Boon3Stefaan Van Gool4Laboratory for Thrombosis Research, Interdisciplinary Research Facility Life Sciences Kulak, E. Sabbelaan 53, 8500 Kortrijk, BelgiumDepartment of Neurosciences, Laboratory of Experimental Neurosurgery and Neuroanatomy, KU Leuven, Herestraat 49, ON1, Box 811, 3000 Leuven, BelgiumLaboratory of Pediatric Immunology, Department of Microbiology and Immunology, KU Leuven, Herestraat 49, ON1, Box 811, 3000 Leuven, BelgiumBioceros B.V., Alexander Numan Building 2nd floor, Yalelaan 46, 3584 CM Utrecht, The NetherlandsLaboratory of Pediatric Immunology, Department of Microbiology and Immunology, KU Leuven, Herestraat 49, ON1, Box 811, 3000 Leuven, BelgiumThe recruitment and activation of regulatory T cells (Tregs) in the micro-environment of malignant brain tumors has detrimental effects on antitumoral immune responses. Hence, local elimination of Tregs within the tumor micro-environment represents a highly valuable tool from both a fundamental and clinical perspective. In the syngeneic experimental GL261 murine glioma model, Tregs were prophylactically eliminated through treatment with PC61, an anti-CD25 mAb. This resulted in specific elimination of CD4+CD25hiFoxp3+ Treg within brain-infiltrating lymphocytes and complete protection against subsequent orthotopic GL261 tumor challenge. Interestingly, PC61-treated mice also showed a pronounced infiltration of CD11b+ myeloid cells in the brain. Phenotypically, these cells could not be considered as Gr-1+ myeloid-derived suppressor cells (MDSC) but were identified as F4/80+ macrophages and granulocytes.http://dx.doi.org/10.1155/2013/952469 |
| spellingShingle | Wim Maes Tina Verschuere Anaïs Van Hoylandt Louis Boon Stefaan Van Gool Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain Clinical and Developmental Immunology |
| title | Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain |
| title_full | Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain |
| title_fullStr | Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain |
| title_full_unstemmed | Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain |
| title_short | Depletion of Regulatory T Cells in a Mouse Experimental Glioma Model through Anti-CD25 Treatment Results in the Infiltration of Non-Immunosuppressive Myeloid Cells in the Brain |
| title_sort | depletion of regulatory t cells in a mouse experimental glioma model through anti cd25 treatment results in the infiltration of non immunosuppressive myeloid cells in the brain |
| url | http://dx.doi.org/10.1155/2013/952469 |
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