Diagnosis of mycoplasmosis in chicks by pathological and real time-pcr methods
<b>Aim:</b> The purpose of this study was to investigate the suitability of the Real Time-PCR in the diagnosis of mycoplasmosis and to determine the pathologic findings in lungs, air sacs, trachea, hearth, liver and kidney tissues.<p> <b>Materials and Methods:</b> Conju...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=852 |
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| Summary: | <b>Aim:</b> The purpose of this study was to investigate the suitability
of the Real Time-PCR in the diagnosis of mycoplasmosis
and to determine the pathologic findings in lungs, air
sacs, trachea, hearth, liver and kidney tissues.<p>
<b>Materials and Methods:</b> Conjunctiva and tracheal swab
samples were taken from broiler chicks with respiratory
disease complaints from 3 different breeders were used.
Ten chicks from three separate flock in each breeders were
collected. Trachea, air sac, lung, liver, kidney, and heart samples
were also collected from the same chicks after necropsy
in order to perform pathological, microbiological and
Real Time-PCR analyses.<p>
<b>Results:</b> Clinically, nasal and conjunctival discharge and
wheezing were observed. Macroscopic examination illustrated
gross catarrhal exudation in trachea and bronchus.
In microscopically, hyperplasia in trachea and bronchus
epithelia, mucus producing cells and mononuclear cellular
infiltration in lamina propria were observed. Mycoplasma
spp. were successfully isolated in the tracheal tissue of 8
broiler chicks. M. gallisepticum specific nucleic acid was
amplified from tissue and swab samples of 22 broiler chicks
by RT-PCR.<p>
<b>Conclusion:</b> RT-PCR seems to be an alternative method
when microbiological analyses are laborious or fails in diagnosis
of mycoplasmosis. |
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| ISSN: | 1309-6958 2146-1953 |