Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracil
Purpose of the study. Was to analyze antitumor efficacy of the XAV 939 Wnt signaling pathway inhibitor and its combination with 5 fluorouracil in subcutaneous xenografts derived from patients with colorectal cancer.Materials and methods. Antitumor efficacy of the agents and their combination was stu...
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| Format: | Article |
| Language: | Russian |
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QUASAR, LLC
2022-03-01
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| Series: | Исследования и практика в медицине |
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| Online Access: | https://www.rpmj.ru/rpmj/article/view/746 |
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| author | A. S. Goncharova A. V. Galina D. V. Khodakova G. Yu. Egorov A. Yu. Maksimov E. N. Kolesnikov E. F. Komarova A. A. Kiblitskaya E. V. Zaikina L. Z. Kurbanova M. V. Mindar |
| author_facet | A. S. Goncharova A. V. Galina D. V. Khodakova G. Yu. Egorov A. Yu. Maksimov E. N. Kolesnikov E. F. Komarova A. A. Kiblitskaya E. V. Zaikina L. Z. Kurbanova M. V. Mindar |
| author_sort | A. S. Goncharova |
| collection | DOAJ |
| description | Purpose of the study. Was to analyze antitumor efficacy of the XAV 939 Wnt signaling pathway inhibitor and its combination with 5 fluorouracil in subcutaneous xenografts derived from patients with colorectal cancer.Materials and methods. Antitumor efficacy of the agents and their combination was studied in xenografts derived from patients with colorectal cancer and subcutaneously implanted in immunodeficient Balb/c Nude mice. All animals with tumors were divided into 4 groups (n = 5): group 1 received 5 fluorouracil 25 mg/kg, group 2 – XAV 939 25 mg/kg, group 3–5 fluorouracil and XAV 939 combination at the same dosages, group 4 was control. Criteria for the efficacy of the tested agents and their combination included tumor growth rate and tumor growth inhibition rate (TGI %).Results. The mean volumes of xenografts and tumor growth rate in the group receiving a combination of 5 fluorouracil and XAV 939 were 335.2 ± 40.7 mm3 , being lower than the averages of xenografts in controls – 609.3 ± 69.5 mm3 (p < 0.05). The mean volumes of xenografts in the group receiving 5 fluorouracil monotherapy were 601.9 ± 45.5 mm3 , in the group with the XAV 939 monotherapy – 527.9 ± 258.6 mm3 . The highest TGI (44.99 %) was registered in the group receiving a combination of 5 fluorouracil and XAV 939.Conclusion. The study revealed the ability of combined XAV 939 Wnt signaling pathway inhibitor and 5 fluorouracil to inhibit the growth of subcutaneous xenografts derived from patients with colorectal cancer. |
| format | Article |
| id | doaj-art-47e3e1fb01e848bf8cf5912a5d63400a |
| institution | Kabale University |
| issn | 2410-1893 |
| language | Russian |
| publishDate | 2022-03-01 |
| publisher | QUASAR, LLC |
| record_format | Article |
| series | Исследования и практика в медицине |
| spelling | doaj-art-47e3e1fb01e848bf8cf5912a5d63400a2025-02-03T00:57:38ZrusQUASAR, LLCИсследования и практика в медицине2410-18932022-03-0191334210.17709/2410-1893-2022-9-1-3429Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracilA. S. Goncharova0A. V. Galina1D. V. Khodakova2G. Yu. Egorov3A. Yu. Maksimov4E. N. Kolesnikov5E. F. Komarova6A. A. Kiblitskaya7E. V. Zaikina8L. Z. Kurbanova9M. V. Mindar10National Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for Oncology; Rostov State Medical UniversityNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyNational Medical Research Centre for OncologyPurpose of the study. Was to analyze antitumor efficacy of the XAV 939 Wnt signaling pathway inhibitor and its combination with 5 fluorouracil in subcutaneous xenografts derived from patients with colorectal cancer.Materials and methods. Antitumor efficacy of the agents and their combination was studied in xenografts derived from patients with colorectal cancer and subcutaneously implanted in immunodeficient Balb/c Nude mice. All animals with tumors were divided into 4 groups (n = 5): group 1 received 5 fluorouracil 25 mg/kg, group 2 – XAV 939 25 mg/kg, group 3–5 fluorouracil and XAV 939 combination at the same dosages, group 4 was control. Criteria for the efficacy of the tested agents and their combination included tumor growth rate and tumor growth inhibition rate (TGI %).Results. The mean volumes of xenografts and tumor growth rate in the group receiving a combination of 5 fluorouracil and XAV 939 were 335.2 ± 40.7 mm3 , being lower than the averages of xenografts in controls – 609.3 ± 69.5 mm3 (p < 0.05). The mean volumes of xenografts in the group receiving 5 fluorouracil monotherapy were 601.9 ± 45.5 mm3 , in the group with the XAV 939 monotherapy – 527.9 ± 258.6 mm3 . The highest TGI (44.99 %) was registered in the group receiving a combination of 5 fluorouracil and XAV 939.Conclusion. The study revealed the ability of combined XAV 939 Wnt signaling pathway inhibitor and 5 fluorouracil to inhibit the growth of subcutaneous xenografts derived from patients with colorectal cancer.https://www.rpmj.ru/rpmj/article/view/746colorectal cancerxenograftmurine modelsin vivo studieswnt signaling pathwaywnt inhibitor |
| spellingShingle | A. S. Goncharova A. V. Galina D. V. Khodakova G. Yu. Egorov A. Yu. Maksimov E. N. Kolesnikov E. F. Komarova A. A. Kiblitskaya E. V. Zaikina L. Z. Kurbanova M. V. Mindar Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracil Исследования и практика в медицине colorectal cancer xenograft murine models in vivo studies wnt signaling pathway wnt inhibitor |
| title | Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracil |
| title_full | Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracil |
| title_fullStr | Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracil |
| title_full_unstemmed | Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracil |
| title_short | Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracil |
| title_sort | inhibition of growth of colorectal cancer patient derived subcutaneous xenografts using combined wnt signaling pathway inhibitor and 5 fluorouracil |
| topic | colorectal cancer xenograft murine models in vivo studies wnt signaling pathway wnt inhibitor |
| url | https://www.rpmj.ru/rpmj/article/view/746 |
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