Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice
Glyphosate (N-(phosphonomethyl) glycine, C3H8NO5P), a herbicide, used to control unwanted annual and perennial plants all over the world. Nevertheless, occupational and environmental exposure to pesticides can pose a threat to nontarget species including human beings. Therefore, in the present study...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2009/308985 |
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| author | Sahdeo Prasad Smita Srivastava Madhulika Singh Yogeshwer Shukla |
| author_facet | Sahdeo Prasad Smita Srivastava Madhulika Singh Yogeshwer Shukla |
| author_sort | Sahdeo Prasad |
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| description | Glyphosate (N-(phosphonomethyl) glycine, C3H8NO5P), a herbicide, used to control unwanted annual and perennial plants all over the world. Nevertheless, occupational and environmental exposure to pesticides can pose a threat to nontarget species including human beings. Therefore, in the present study, genotoxic effects of the herbicide glyphosate were analyzed by measuring chromosomal aberrations (CAs) and micronuclei (MN) in bone marrow cells of Swiss albino mice. A single dose of glyphosate was given intraperitoneally (i.p) to the animals at a concentration
of 25 and 50 mg/kg b.wt. Animals of positive control group were injected i.p. benzo(a)pyrene
(100 mg/kg b.wt., once only), whereas, animals of control (vehicle) group were injected i.p. dimethyl sulfoxide (0.2 mL). Animals from all the groups were sacrificed at sampling times of 24, 48, and 72 hours and their bone marrow was analyzed for cytogenetic and chromosomal damage. Glyphosate treatment significantly increases CAs and MN induction at both treatments and time compared with the vehicle control (P<.05). The cytotoxic effects of glyphosate were also evident, as observed by significant decrease in mitotic index (MI). The present results indicate that glyphosate is clastogenic and cytotoxic to mouse bone marrow. |
| format | Article |
| id | doaj-art-47e0cf3d44eb4139a51ab390f93215f1 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
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| series | Journal of Toxicology |
| spelling | doaj-art-47e0cf3d44eb4139a51ab390f93215f12025-02-03T01:02:17ZengWileyJournal of Toxicology1687-81911687-82052009-01-01200910.1155/2009/308985308985Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino MiceSahdeo Prasad0Smita Srivastava1Madhulika Singh2Yogeshwer Shukla3Proteomics Laboratory, Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow 226001, IndiaProteomics Laboratory, Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow 226001, IndiaProteomics Laboratory, Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow 226001, IndiaProteomics Laboratory, Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow 226001, IndiaGlyphosate (N-(phosphonomethyl) glycine, C3H8NO5P), a herbicide, used to control unwanted annual and perennial plants all over the world. Nevertheless, occupational and environmental exposure to pesticides can pose a threat to nontarget species including human beings. Therefore, in the present study, genotoxic effects of the herbicide glyphosate were analyzed by measuring chromosomal aberrations (CAs) and micronuclei (MN) in bone marrow cells of Swiss albino mice. A single dose of glyphosate was given intraperitoneally (i.p) to the animals at a concentration of 25 and 50 mg/kg b.wt. Animals of positive control group were injected i.p. benzo(a)pyrene (100 mg/kg b.wt., once only), whereas, animals of control (vehicle) group were injected i.p. dimethyl sulfoxide (0.2 mL). Animals from all the groups were sacrificed at sampling times of 24, 48, and 72 hours and their bone marrow was analyzed for cytogenetic and chromosomal damage. Glyphosate treatment significantly increases CAs and MN induction at both treatments and time compared with the vehicle control (P<.05). The cytotoxic effects of glyphosate were also evident, as observed by significant decrease in mitotic index (MI). The present results indicate that glyphosate is clastogenic and cytotoxic to mouse bone marrow.http://dx.doi.org/10.1155/2009/308985 |
| spellingShingle | Sahdeo Prasad Smita Srivastava Madhulika Singh Yogeshwer Shukla Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice Journal of Toxicology |
| title | Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice |
| title_full | Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice |
| title_fullStr | Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice |
| title_full_unstemmed | Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice |
| title_short | Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice |
| title_sort | clastogenic effects of glyphosate in bone marrow cells of swiss albino mice |
| url | http://dx.doi.org/10.1155/2009/308985 |
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