Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat
IntroductionUnderstanding the dynamics of Pb tolerance across different growth stages is essential for breeding wheat varieties with comprehensive tolerance to Pb contamination throughout their life cycle. Lead (Pb) in soil affects wheat plants at all growth stages. However, the seedling stage is co...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Agronomy |
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| author | Amira M. I. Mourad Amira M. I. Mourad Ahmed Sallam Ahmed Sallam Khaled A. Farghaly Andreas Börner |
| author_facet | Amira M. I. Mourad Amira M. I. Mourad Ahmed Sallam Ahmed Sallam Khaled A. Farghaly Andreas Börner |
| author_sort | Amira M. I. Mourad |
| collection | DOAJ |
| description | IntroductionUnderstanding the dynamics of Pb tolerance across different growth stages is essential for breeding wheat varieties with comprehensive tolerance to Pb contamination throughout their life cycle. Lead (Pb) in soil affects wheat plants at all growth stages. However, the seedling stage is considered a sensitive growth stage for the tolerance of most abiotic stresses. Genetic variation in lead tolerance is poorly understood, and genetic control has still not been investigated.MaterialsIn this study, 103 highly diverse wheat genotypes were investigated under normal and lead stress conditions at the seedling stage. Different seedling growth traits were scored under each treatment. GWAS was performed via two different sets of markers: 21,750 (25K Infinium iSelect array) and 37,790 SNPs (genotyping-by-sequencing) to identify SNP markers associated with the studied seedling traits.ResultsHigh genetic variation was found among all the genotypes for all the traits. High broad-sense heritability estimates ranging from 0.25-0.93 were observed. A highly significant correlation was found among all the traits scored under normal and lead stress conditions. Low or no significant phenotypic correlations were found for lead tolerance between the seedling and adult growth stages. A set of eight genotypes were classified as lead-tolerant genotypes. The GWAS revealed a set of 222 significant markers associated with lead tolerance. Markers with pleiotropic effects were detected under Pb stress and between lead and normal conditions. Interestingly, five significant markers were found to be associated with lead tolerance at the seedling and adult growth stages.DiscussionThe results of this study provide new and novel insights into genetic control and genetic variation in lead tolerance at the early growth stage. All the genes and genotypes reported in this study will be very useful for further investigations of lead tolerance in wheat at different growth stages. |
| format | Article |
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| institution | Kabale University |
| issn | 2673-3218 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Agronomy |
| spelling | doaj-art-47d59a84b1f5439f8ad277b86be40e412025-02-06T05:21:51ZengFrontiers Media S.A.Frontiers in Agronomy2673-32182025-02-01710.3389/fagro.2025.14283661428366Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheatAmira M. I. Mourad0Amira M. I. Mourad1Ahmed Sallam2Ahmed Sallam3Khaled A. Farghaly4Andreas Börner5Genebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyDepartment of Agronomy, Faculty of Agriculture, Assiut University, Assiut, EgyptGenebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyDepartment of Genetics, Faculty of Agriculture, Assiut University, Assiut, EgyptDepartment of Soil and Water Resources, Faculty of Agriculture, Assiut University, Assiut, EgyptGenebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyIntroductionUnderstanding the dynamics of Pb tolerance across different growth stages is essential for breeding wheat varieties with comprehensive tolerance to Pb contamination throughout their life cycle. Lead (Pb) in soil affects wheat plants at all growth stages. However, the seedling stage is considered a sensitive growth stage for the tolerance of most abiotic stresses. Genetic variation in lead tolerance is poorly understood, and genetic control has still not been investigated.MaterialsIn this study, 103 highly diverse wheat genotypes were investigated under normal and lead stress conditions at the seedling stage. Different seedling growth traits were scored under each treatment. GWAS was performed via two different sets of markers: 21,750 (25K Infinium iSelect array) and 37,790 SNPs (genotyping-by-sequencing) to identify SNP markers associated with the studied seedling traits.ResultsHigh genetic variation was found among all the genotypes for all the traits. High broad-sense heritability estimates ranging from 0.25-0.93 were observed. A highly significant correlation was found among all the traits scored under normal and lead stress conditions. Low or no significant phenotypic correlations were found for lead tolerance between the seedling and adult growth stages. A set of eight genotypes were classified as lead-tolerant genotypes. The GWAS revealed a set of 222 significant markers associated with lead tolerance. Markers with pleiotropic effects were detected under Pb stress and between lead and normal conditions. Interestingly, five significant markers were found to be associated with lead tolerance at the seedling and adult growth stages.DiscussionThe results of this study provide new and novel insights into genetic control and genetic variation in lead tolerance at the early growth stage. All the genes and genotypes reported in this study will be very useful for further investigations of lead tolerance in wheat at different growth stages.https://www.frontiersin.org/articles/10.3389/fagro.2025.1428366/fullheavy metalsseedling tolerancegenome-wide association studygene enrichmentfunctional annotationgene network |
| spellingShingle | Amira M. I. Mourad Amira M. I. Mourad Ahmed Sallam Ahmed Sallam Khaled A. Farghaly Andreas Börner Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat Frontiers in Agronomy heavy metals seedling tolerance genome-wide association study gene enrichment functional annotation gene network |
| title | Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat |
| title_full | Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat |
| title_fullStr | Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat |
| title_full_unstemmed | Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat |
| title_short | Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat |
| title_sort | detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat |
| topic | heavy metals seedling tolerance genome-wide association study gene enrichment functional annotation gene network |
| url | https://www.frontiersin.org/articles/10.3389/fagro.2025.1428366/full |
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