MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injury
Objectives: Deep-venous thrombosis (DVT) refers to abnormal blood clotting in the deep vein cavity, and post-thrombotic syndrome (PTS) is the most frequent complication. The study explored the impact of microRNA 181b-5p on DVT progression based on human umbilical vein endothelial cells (HUVECs).Meth...
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Taylor & Francis Group
2024-12-01
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| Series: | Hematology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16078454.2024.2423438 |
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| author | Dawei Zhang Cheng Cheng Meiying Yang Xiuyin Zhang Xinming Yu Min Wang |
| author_facet | Dawei Zhang Cheng Cheng Meiying Yang Xiuyin Zhang Xinming Yu Min Wang |
| author_sort | Dawei Zhang |
| collection | DOAJ |
| description | Objectives: Deep-venous thrombosis (DVT) refers to abnormal blood clotting in the deep vein cavity, and post-thrombotic syndrome (PTS) is the most frequent complication. The study explored the impact of microRNA 181b-5p on DVT progression based on human umbilical vein endothelial cells (HUVECs).Methods: Levels of miR-181b-5p were examined in 150 cases with acute lower extremity DVT. ROC curve and K-M plot were drawn for clinical value assessment. The role of miR-181b-5p in HUVECs viability, migration, apoptosis, inflammatory response and adhesion factors’ release was investigated. Target gene of miR-181b-5p was predicted, and its role in cell function was explored.Results: Low-expressed miR-181b-5p showed favorable diagnostic performance in differentiating DVT with the AUC of 0.948. Patients with low miR-181b-5p had a high incidence of PTS. miR-181b-5p overexpression promoted HUVECs’ viability and migration, while inhibiting cell apoptosis and release of inflammatory and adhesion cytokines. As the target gene of miR-181b-5p, HEY2 overexpression reversed the role of miR-181b-5p in HUVECs.Conclusion: MiR-181b-5p serves as a potential biomarker for DVT diagnosis and PTS development. Overexpression of this miRNA targeted HEY2 to alleviate endothelial cell damage. |
| format | Article |
| id | doaj-art-47cfdcd2cf5e4e70a65d174fd6e2bf7c |
| institution | OA Journals |
| issn | 1607-8454 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Hematology |
| spelling | doaj-art-47cfdcd2cf5e4e70a65d174fd6e2bf7c2025-08-20T02:34:31ZengTaylor & Francis GroupHematology1607-84542024-12-0129110.1080/16078454.2024.2423438MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injuryDawei Zhang0Cheng Cheng1Meiying Yang2Xiuyin Zhang3Xinming Yu4Min Wang5Department of Orthopedics, Zibo Central Hospital, Zibo, People’s Republic of ChinaDepartment of Cardiovascular Medicine, Zibo Central Hospital, Zibo, People’s Republic of ChinaDepartment of Orthopedics, Zibo Central Hospital, Zibo, People’s Republic of ChinaDepartment of Burn Plastic Surgery, Zibo Central Hospital, Zibo, People’s Republic of ChinaDepartment of Vascular Surgery, Zibo Central Hospital, Zibo, People’s Republic of ChinaDepartment of Vascular Surgery, Zibo Central Hospital, Zibo, People’s Republic of ChinaObjectives: Deep-venous thrombosis (DVT) refers to abnormal blood clotting in the deep vein cavity, and post-thrombotic syndrome (PTS) is the most frequent complication. The study explored the impact of microRNA 181b-5p on DVT progression based on human umbilical vein endothelial cells (HUVECs).Methods: Levels of miR-181b-5p were examined in 150 cases with acute lower extremity DVT. ROC curve and K-M plot were drawn for clinical value assessment. The role of miR-181b-5p in HUVECs viability, migration, apoptosis, inflammatory response and adhesion factors’ release was investigated. Target gene of miR-181b-5p was predicted, and its role in cell function was explored.Results: Low-expressed miR-181b-5p showed favorable diagnostic performance in differentiating DVT with the AUC of 0.948. Patients with low miR-181b-5p had a high incidence of PTS. miR-181b-5p overexpression promoted HUVECs’ viability and migration, while inhibiting cell apoptosis and release of inflammatory and adhesion cytokines. As the target gene of miR-181b-5p, HEY2 overexpression reversed the role of miR-181b-5p in HUVECs.Conclusion: MiR-181b-5p serves as a potential biomarker for DVT diagnosis and PTS development. Overexpression of this miRNA targeted HEY2 to alleviate endothelial cell damage.https://www.tandfonline.com/doi/10.1080/16078454.2024.2423438miR-181b-5pdeep-venous thrombosishuman umbilical vein endothelial cellspost-thrombotic syndrome |
| spellingShingle | Dawei Zhang Cheng Cheng Meiying Yang Xiuyin Zhang Xinming Yu Min Wang MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injury Hematology miR-181b-5p deep-venous thrombosis human umbilical vein endothelial cells post-thrombotic syndrome |
| title | MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injury |
| title_full | MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injury |
| title_fullStr | MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injury |
| title_full_unstemmed | MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injury |
| title_short | MicroRNA-181b-5p/HEY2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injury |
| title_sort | microrna 181b 5p hey2 axis is involved in the progress of deep venous thrombosis via mediating vascular endothelial injury |
| topic | miR-181b-5p deep-venous thrombosis human umbilical vein endothelial cells post-thrombotic syndrome |
| url | https://www.tandfonline.com/doi/10.1080/16078454.2024.2423438 |
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