Bone Formation with Deproteinized Bovine Bone Mineral or Biphasic Calcium Phosphate in the Presence of Autologous Platelet Lysate: Comparative Investigation in Rabbit
Bone substitutes alone or supplemented with platelet-derived concentrates are widely used to promote bone regeneration but their potency remains controversial. The aim of this study was, therefore, to compare the regenerative potential of preparations containing autologous platelet lysate (APL) and...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2014/367265 |
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| author | Carole Chakar Nada Naaman Emmanuel Soffer Nicolas Cohen Nada El Osta Hervé Petite Fani Anagnostou |
| author_facet | Carole Chakar Nada Naaman Emmanuel Soffer Nicolas Cohen Nada El Osta Hervé Petite Fani Anagnostou |
| author_sort | Carole Chakar |
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| description | Bone substitutes alone or supplemented with platelet-derived concentrates are widely used to promote bone regeneration but their potency remains controversial. The aim of this study was, therefore, to compare the regenerative potential of preparations containing autologous platelet lysate (APL) and particles of either deproteinized bovine bone mineral (DBBM) or biphasic calcium phosphate (BCP), two bone substitutes with different resorption patterns. Rabbit APL was prepared by freeze-thawing a platelet suspension. Critical-size defects in rabbit femoral condyle were filled with DBBM or DBBM+APL and BCP or BCP+APL. Rabbits were sacrificed after six weeks and newly formed bone and residual implanted material were evaluated using nondemineralized histology and histomorphometry. New bone was observed around particles of all fillers tested. In the defects filled with BCP, the newly formed bone area was greater (70%; P<0.001) while the residual material area was lower (60%; P<0.001) than that observed in those filled with DBBM. New bone and residual material area of defects filled with either APL+DBBM or APL+BCP were similar to those observed in those filled with the material alone. In summary, osteoconductivity and resorption of BCP were greater than those of DBBM, while APL associated with either DBBM or BCP did not have an additional benefit. |
| format | Article |
| id | doaj-art-47c7dfba1d414eb592bc9eda75705b88 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | International Journal of Biomaterials |
| spelling | doaj-art-47c7dfba1d414eb592bc9eda75705b882025-02-03T01:02:55ZengWileyInternational Journal of Biomaterials1687-87871687-87952014-01-01201410.1155/2014/367265367265Bone Formation with Deproteinized Bovine Bone Mineral or Biphasic Calcium Phosphate in the Presence of Autologous Platelet Lysate: Comparative Investigation in RabbitCarole Chakar0Nada Naaman1Emmanuel Soffer2Nicolas Cohen3Nada El Osta4Hervé Petite5Fani Anagnostou6Department of Periodontology, School of Dentistry, Saint-Joseph University, P.O. Box 11-5076, Riad el Solh, Beirut 1107 2050, LebanonDepartment of Periodontology, School of Dentistry, Saint-Joseph University, P.O. Box 11-5076, Riad el Solh, Beirut 1107 2050, LebanonLaboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, FranceLaboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, FranceDepartment of Prosthetic Dentistry, School of Dentistry, Saint-Joseph University, P.O. Box 11-5076, Riad el Solh, Beirut 1107 2050, LebanonLaboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, FranceLaboratoire de Bioingénierie et Biomécanique Ostéo-Articulaires, UMR CNRS 7052, PRES Sorbonne Paris Cité, 75010 Paris, FranceBone substitutes alone or supplemented with platelet-derived concentrates are widely used to promote bone regeneration but their potency remains controversial. The aim of this study was, therefore, to compare the regenerative potential of preparations containing autologous platelet lysate (APL) and particles of either deproteinized bovine bone mineral (DBBM) or biphasic calcium phosphate (BCP), two bone substitutes with different resorption patterns. Rabbit APL was prepared by freeze-thawing a platelet suspension. Critical-size defects in rabbit femoral condyle were filled with DBBM or DBBM+APL and BCP or BCP+APL. Rabbits were sacrificed after six weeks and newly formed bone and residual implanted material were evaluated using nondemineralized histology and histomorphometry. New bone was observed around particles of all fillers tested. In the defects filled with BCP, the newly formed bone area was greater (70%; P<0.001) while the residual material area was lower (60%; P<0.001) than that observed in those filled with DBBM. New bone and residual material area of defects filled with either APL+DBBM or APL+BCP were similar to those observed in those filled with the material alone. In summary, osteoconductivity and resorption of BCP were greater than those of DBBM, while APL associated with either DBBM or BCP did not have an additional benefit.http://dx.doi.org/10.1155/2014/367265 |
| spellingShingle | Carole Chakar Nada Naaman Emmanuel Soffer Nicolas Cohen Nada El Osta Hervé Petite Fani Anagnostou Bone Formation with Deproteinized Bovine Bone Mineral or Biphasic Calcium Phosphate in the Presence of Autologous Platelet Lysate: Comparative Investigation in Rabbit International Journal of Biomaterials |
| title | Bone Formation with Deproteinized Bovine Bone Mineral or Biphasic Calcium Phosphate in the Presence of Autologous Platelet Lysate: Comparative Investigation in Rabbit |
| title_full | Bone Formation with Deproteinized Bovine Bone Mineral or Biphasic Calcium Phosphate in the Presence of Autologous Platelet Lysate: Comparative Investigation in Rabbit |
| title_fullStr | Bone Formation with Deproteinized Bovine Bone Mineral or Biphasic Calcium Phosphate in the Presence of Autologous Platelet Lysate: Comparative Investigation in Rabbit |
| title_full_unstemmed | Bone Formation with Deproteinized Bovine Bone Mineral or Biphasic Calcium Phosphate in the Presence of Autologous Platelet Lysate: Comparative Investigation in Rabbit |
| title_short | Bone Formation with Deproteinized Bovine Bone Mineral or Biphasic Calcium Phosphate in the Presence of Autologous Platelet Lysate: Comparative Investigation in Rabbit |
| title_sort | bone formation with deproteinized bovine bone mineral or biphasic calcium phosphate in the presence of autologous platelet lysate comparative investigation in rabbit |
| url | http://dx.doi.org/10.1155/2014/367265 |
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