INVESTIGATION OF THE SPATIAL GENOME ORGANIZATION OF MOUSE SPERM AND FIBROBLASTS BY THE Hi-C METHOD
The spatial organization of an eukaryotic genome plays an important role in the control of nuclear gene expression. The new Hi-C method allows investigation of the three-dimensional architecture of whole genomes. It has not been applied to study of the spatial configuration of a germ cell genome hit...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
|
| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/256 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575291469332480 |
|---|---|
| author | N. R. Battulin V. S. Fishman A. A. Khabarova M. Yu. Pomaznoy T. A. Shnaider D. A. Afonnikov O. L. Serov |
| author_facet | N. R. Battulin V. S. Fishman A. A. Khabarova M. Yu. Pomaznoy T. A. Shnaider D. A. Afonnikov O. L. Serov |
| author_sort | N. R. Battulin |
| collection | DOAJ |
| description | The spatial organization of an eukaryotic genome plays an important role in the control of nuclear gene expression. The new Hi-C method allows investigation of the three-dimensional architecture of whole genomes. It has not been applied to study of the spatial configuration of a germ cell genome hitherto. Here we describe a protocol for production and quality control of Hi-C libraries from fibroblasts and sperm cells. Our results demonstrate that the Hi-C method can be used for studying the spatial organization of the densely packed sperm genome. |
| format | Article |
| id | doaj-art-47a9c844d85749fbba4343eeee8f557d |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-47a9c844d85749fbba4343eeee8f557d2025-02-01T09:58:00ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01182338344240INVESTIGATION OF THE SPATIAL GENOME ORGANIZATION OF MOUSE SPERM AND FIBROBLASTS BY THE Hi-C METHODN. R. Battulin0V. S. Fishman1A. A. Khabarova2M. Yu. Pomaznoy3T. A. Shnaider4D. A. Afonnikov5O. L. Serov6Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, Russia Novosibirsk National Research State University, Novosibirsk, RussiaThe spatial organization of an eukaryotic genome plays an important role in the control of nuclear gene expression. The new Hi-C method allows investigation of the three-dimensional architecture of whole genomes. It has not been applied to study of the spatial configuration of a germ cell genome hitherto. Here we describe a protocol for production and quality control of Hi-C libraries from fibroblasts and sperm cells. Our results demonstrate that the Hi-C method can be used for studying the spatial organization of the densely packed sperm genome.https://vavilov.elpub.ru/jour/article/view/256spatial genome organizationhi-cgerm cellssomatic cells |
| spellingShingle | N. R. Battulin V. S. Fishman A. A. Khabarova M. Yu. Pomaznoy T. A. Shnaider D. A. Afonnikov O. L. Serov INVESTIGATION OF THE SPATIAL GENOME ORGANIZATION OF MOUSE SPERM AND FIBROBLASTS BY THE Hi-C METHOD Вавиловский журнал генетики и селекции spatial genome organization hi-c germ cells somatic cells |
| title | INVESTIGATION OF THE SPATIAL GENOME ORGANIZATION OF MOUSE SPERM AND FIBROBLASTS BY THE Hi-C METHOD |
| title_full | INVESTIGATION OF THE SPATIAL GENOME ORGANIZATION OF MOUSE SPERM AND FIBROBLASTS BY THE Hi-C METHOD |
| title_fullStr | INVESTIGATION OF THE SPATIAL GENOME ORGANIZATION OF MOUSE SPERM AND FIBROBLASTS BY THE Hi-C METHOD |
| title_full_unstemmed | INVESTIGATION OF THE SPATIAL GENOME ORGANIZATION OF MOUSE SPERM AND FIBROBLASTS BY THE Hi-C METHOD |
| title_short | INVESTIGATION OF THE SPATIAL GENOME ORGANIZATION OF MOUSE SPERM AND FIBROBLASTS BY THE Hi-C METHOD |
| title_sort | investigation of the spatial genome organization of mouse sperm and fibroblasts by the hi c method |
| topic | spatial genome organization hi-c germ cells somatic cells |
| url | https://vavilov.elpub.ru/jour/article/view/256 |
| work_keys_str_mv | AT nrbattulin investigationofthespatialgenomeorganizationofmousespermandfibroblastsbythehicmethod AT vsfishman investigationofthespatialgenomeorganizationofmousespermandfibroblastsbythehicmethod AT aakhabarova investigationofthespatialgenomeorganizationofmousespermandfibroblastsbythehicmethod AT myupomaznoy investigationofthespatialgenomeorganizationofmousespermandfibroblastsbythehicmethod AT tashnaider investigationofthespatialgenomeorganizationofmousespermandfibroblastsbythehicmethod AT daafonnikov investigationofthespatialgenomeorganizationofmousespermandfibroblastsbythehicmethod AT olserov investigationofthespatialgenomeorganizationofmousespermandfibroblastsbythehicmethod |