In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and Proliferation
Purpose. To evaluate the dose effect of vitamin K3 on wound healing mechanisms. Methods. Conjunctival fibroblasts were incubated for 24 hours. An artificial wound was made and the cells were incubated with fresh medium plus doses of vitamin K3 to be tested. Wound repair was monitored at 0, 18, 24, a...
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/916713 |
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| author | I. Pinilla L. B. Izaguirre F. J. Gonzalvo E. Piazuelo M. A. Garcia-Gonzalez A. I. Sanchez-Cano F. Sopeña |
| author_facet | I. Pinilla L. B. Izaguirre F. J. Gonzalvo E. Piazuelo M. A. Garcia-Gonzalez A. I. Sanchez-Cano F. Sopeña |
| author_sort | I. Pinilla |
| collection | DOAJ |
| description | Purpose. To evaluate the dose effect of vitamin K3 on wound healing mechanisms. Methods. Conjunctival fibroblasts were incubated for 24 hours. An artificial wound was made and the cells were incubated with fresh medium plus doses of vitamin K3 to be tested. Wound repair was monitored at 0, 18, 24, and 48 hours. Proliferation was measured in actively dividing cells by [3H]thymidine uptake. Six different groups were tested: group 1/no drugs added, group 2/ethanol 0.1%, group 3/vitamin K3 1 mg/L, group 4/vitamin K3 2 mg/L, group 5/vitamin K3 4 mg/L, and group 6/vitamin K3 6 mg/L. Each experiment was carried out in triplicate and 4 times. Results. There were no differences among groups at the initial time. In vitro wound repair was slower in groups 4, 5, and 6. There were no differences between control and ethanol groups and between control and vitamin K3 1 mg/L groups. Fibroblast mitogenic activity was statistically decreased in all vitamin K groups; statistical differences were found among vitamin K3 1 mg/mL and higher doses too. In groups 5 and 6, cellular toxicity was presented. Conclusions. Vitamin K3 is able to inhibit fibroblast proliferation. Vitamin K3 2 mg/L or higher doses inhibit wound healing repair, exhibiting cellular toxicity at 4 and 6 mg/L. |
| format | Article |
| id | doaj-art-47a38f5955454003b521fba413373822 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-47a38f5955454003b521fba4133738222025-02-03T01:03:08ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/916713916713In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and ProliferationI. Pinilla0L. B. Izaguirre1F. J. Gonzalvo2E. Piazuelo3M. A. Garcia-Gonzalez4A. I. Sanchez-Cano5F. Sopeña6Department of Ophthalmology, Lozano Blesa University Hospital, C/San Juan Bosco 15, 50009 Zaragoza, SpainDepartment of Ophthalmology, Hospital García Orcoyen, Navarra, 31200 Estella, SpainDepartment of Ophthalmology, Complejo Hospitalario de Navarra, Navarra, 31008 Pamplona, SpainAragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, SpainAragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, SpainAragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, SpainAragon Institute of Health Sciences (IIS Aragon), 50009 Zaragoza, SpainPurpose. To evaluate the dose effect of vitamin K3 on wound healing mechanisms. Methods. Conjunctival fibroblasts were incubated for 24 hours. An artificial wound was made and the cells were incubated with fresh medium plus doses of vitamin K3 to be tested. Wound repair was monitored at 0, 18, 24, and 48 hours. Proliferation was measured in actively dividing cells by [3H]thymidine uptake. Six different groups were tested: group 1/no drugs added, group 2/ethanol 0.1%, group 3/vitamin K3 1 mg/L, group 4/vitamin K3 2 mg/L, group 5/vitamin K3 4 mg/L, and group 6/vitamin K3 6 mg/L. Each experiment was carried out in triplicate and 4 times. Results. There were no differences among groups at the initial time. In vitro wound repair was slower in groups 4, 5, and 6. There were no differences between control and ethanol groups and between control and vitamin K3 1 mg/L groups. Fibroblast mitogenic activity was statistically decreased in all vitamin K groups; statistical differences were found among vitamin K3 1 mg/mL and higher doses too. In groups 5 and 6, cellular toxicity was presented. Conclusions. Vitamin K3 is able to inhibit fibroblast proliferation. Vitamin K3 2 mg/L or higher doses inhibit wound healing repair, exhibiting cellular toxicity at 4 and 6 mg/L.http://dx.doi.org/10.1155/2014/916713 |
| spellingShingle | I. Pinilla L. B. Izaguirre F. J. Gonzalvo E. Piazuelo M. A. Garcia-Gonzalez A. I. Sanchez-Cano F. Sopeña In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and Proliferation The Scientific World Journal |
| title | In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and Proliferation |
| title_full | In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and Proliferation |
| title_fullStr | In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and Proliferation |
| title_full_unstemmed | In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and Proliferation |
| title_short | In Vitro Vitamin K3 Effect on Conjunctival Fibroblast Migration and Proliferation |
| title_sort | in vitro vitamin k3 effect on conjunctival fibroblast migration and proliferation |
| url | http://dx.doi.org/10.1155/2014/916713 |
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