Narciclasine inhibits vaccinia virus infection by activating the RhoA signaling pathway
In 2022, a sharp rise in global cases of mpox virus (MPXV) led the World Health Organization (WHO) to declare it a public health emergency of international concern. However, progress in developing drugs targeting MPXV has been slow. Here, we investigate the natural alkaloid narciclasine as a potenti...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Biosafety and Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590053624001332 |
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| author | Ting Xu Zhengyang Pan Xue Li Mengyang Zhao Zichen Li Leiliang Zhang |
| author_facet | Ting Xu Zhengyang Pan Xue Li Mengyang Zhao Zichen Li Leiliang Zhang |
| author_sort | Ting Xu |
| collection | DOAJ |
| description | In 2022, a sharp rise in global cases of mpox virus (MPXV) led the World Health Organization (WHO) to declare it a public health emergency of international concern. However, progress in developing drugs targeting MPXV has been slow. Here, we investigate the natural alkaloid narciclasine as a potential inhibitor of poxviruses. Our investigation demonstrates that narciclasine at 40 nmol/L (nM) to 160 nM dosages effectively blocks vaccinia virus (VACV), a representative poxvirus. Specifically, narciclasine disrupts the production of extracellular enveloped virus (EEV), which is crucial for viral spread. Narciclasine’s antiviral impact is probably attributed to its activation of the RhoA signaling pathway. This study highlights narciclasine’s potential as a promising new therapeutic candidate against poxviruses, offering prospects for its development into a potent antiviral agent that is essential for combating emerging poxvirus outbreaks. |
| format | Article |
| id | doaj-art-4785c6c82b334066b0410bb965a8957d |
| institution | DOAJ |
| issn | 2590-0536 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosafety and Health |
| spelling | doaj-art-4785c6c82b334066b0410bb965a8957d2025-08-20T02:57:35ZengElsevierBiosafety and Health2590-05362024-12-016634134910.1016/j.bsheal.2024.11.002Narciclasine inhibits vaccinia virus infection by activating the RhoA signaling pathwayTing Xu0Zhengyang Pan1Xue Li2Mengyang Zhao3Zichen Li4Leiliang Zhang5Department of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan 250014, China; Department of Pathogen Biology, School of Clinical and Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, ChinaDepartment of Pathogen Biology, School of Clinical and Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, ChinaDepartment of Pathogen Biology, School of Clinical and Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, ChinaDepartment of Pathogen Biology, School of Clinical and Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, ChinaDepartment of Pathogen Biology, School of Clinical and Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, ChinaDepartment of Clinical Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan 250014, China; Department of Pathogen Biology, School of Clinical and Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China; Corresponding author: Department of Pathogen Biology, School of Clinical and Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250117, China.In 2022, a sharp rise in global cases of mpox virus (MPXV) led the World Health Organization (WHO) to declare it a public health emergency of international concern. However, progress in developing drugs targeting MPXV has been slow. Here, we investigate the natural alkaloid narciclasine as a potential inhibitor of poxviruses. Our investigation demonstrates that narciclasine at 40 nmol/L (nM) to 160 nM dosages effectively blocks vaccinia virus (VACV), a representative poxvirus. Specifically, narciclasine disrupts the production of extracellular enveloped virus (EEV), which is crucial for viral spread. Narciclasine’s antiviral impact is probably attributed to its activation of the RhoA signaling pathway. This study highlights narciclasine’s potential as a promising new therapeutic candidate against poxviruses, offering prospects for its development into a potent antiviral agent that is essential for combating emerging poxvirus outbreaks.http://www.sciencedirect.com/science/article/pii/S2590053624001332Mpox virus (MPXV)Vaccinia virus (VACA)NarciclasineRhoAExtracellular enveloped virus (EEV) |
| spellingShingle | Ting Xu Zhengyang Pan Xue Li Mengyang Zhao Zichen Li Leiliang Zhang Narciclasine inhibits vaccinia virus infection by activating the RhoA signaling pathway Biosafety and Health Mpox virus (MPXV) Vaccinia virus (VACA) Narciclasine RhoA Extracellular enveloped virus (EEV) |
| title | Narciclasine inhibits vaccinia virus infection by activating the RhoA signaling pathway |
| title_full | Narciclasine inhibits vaccinia virus infection by activating the RhoA signaling pathway |
| title_fullStr | Narciclasine inhibits vaccinia virus infection by activating the RhoA signaling pathway |
| title_full_unstemmed | Narciclasine inhibits vaccinia virus infection by activating the RhoA signaling pathway |
| title_short | Narciclasine inhibits vaccinia virus infection by activating the RhoA signaling pathway |
| title_sort | narciclasine inhibits vaccinia virus infection by activating the rhoa signaling pathway |
| topic | Mpox virus (MPXV) Vaccinia virus (VACA) Narciclasine RhoA Extracellular enveloped virus (EEV) |
| url | http://www.sciencedirect.com/science/article/pii/S2590053624001332 |
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