Increased IL-21 Expression Induces Granzyme B in Peripheral CD5+ B Cells as a Potential Counter-Regulatory Effect in Primary Sjögren’s Syndrome
Recently, we reported elevated proportions of circulating follicular T helper cells and higher levels of interleukin- (IL-) 21 in primary Sjögren’s syndrome (pSS). Interaction of invariant natural killer T (iNKT) cells with B cells and granzyme B (GrB) production may be also important in pSS. Thirty...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/4328372 |
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| author | Gábor Papp Edit Gyimesi Krisztina Szabó Éva Zöld Margit Zeher |
| author_facet | Gábor Papp Edit Gyimesi Krisztina Szabó Éva Zöld Margit Zeher |
| author_sort | Gábor Papp |
| collection | DOAJ |
| description | Recently, we reported elevated proportions of circulating follicular T helper cells and higher levels of interleukin- (IL-) 21 in primary Sjögren’s syndrome (pSS). Interaction of invariant natural killer T (iNKT) cells with B cells and granzyme B (GrB) production may be also important in pSS. Thirty-two pSS patients and 24 healthy controls were enrolled in our study. We investigated the expression of intracellular GrB and IL-21 receptor (IL-21R) of CD19+CD5+ and CD19+CD5− B cells; furthermore, we determined the IL-21 expression of iNKT cells as well. We also assessed the proportion of transitional (CD19+CD24highCD38high), mature (CD19+CD24intCD38int) and primarily memory (CD19+CD24highCD38-) B cells. CD5+ but not CD5− B cells showed elevated GrB and IL-21R expression in pSS; additionally IL-21 expression of iNKT cells was also elevated. The ratios of transitional and mature B cells were elevated in pSS, while primarily memory B cell percentages were decreased, which correlated with GrB and IL-21R expression of CD19+ B cells. Our results suggest that enhanced IL-21R expression of CD19+CD5+ B cells and production of IL-21 by iNKT cells may play an important role in the pathogenesis of pSS by regulating CD19+CD5+ B cell functions and increasing GrB production, presumably leading to a counter-regulatory effect in the disease. |
| format | Article |
| id | doaj-art-476cb5835f384695a61dd3c8af5eb281 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-476cb5835f384695a61dd3c8af5eb2812025-02-03T01:26:29ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/43283724328372Increased IL-21 Expression Induces Granzyme B in Peripheral CD5+ B Cells as a Potential Counter-Regulatory Effect in Primary Sjögren’s SyndromeGábor Papp0Edit Gyimesi1Krisztina Szabó2Éva Zöld3Margit Zeher4Division of Clinical Immunology, Faculty of Medicine, University of Debrecen, Móricz Zsigmond Street 22, Debrecen 4032, HungaryDivision of Clinical Immunology, Faculty of Medicine, University of Debrecen, Móricz Zsigmond Street 22, Debrecen 4032, HungaryDivision of Clinical Immunology, Faculty of Medicine, University of Debrecen, Móricz Zsigmond Street 22, Debrecen 4032, HungaryDivision of Clinical Immunology, Faculty of Medicine, University of Debrecen, Móricz Zsigmond Street 22, Debrecen 4032, HungaryDivision of Clinical Immunology, Faculty of Medicine, University of Debrecen, Móricz Zsigmond Street 22, Debrecen 4032, HungaryRecently, we reported elevated proportions of circulating follicular T helper cells and higher levels of interleukin- (IL-) 21 in primary Sjögren’s syndrome (pSS). Interaction of invariant natural killer T (iNKT) cells with B cells and granzyme B (GrB) production may be also important in pSS. Thirty-two pSS patients and 24 healthy controls were enrolled in our study. We investigated the expression of intracellular GrB and IL-21 receptor (IL-21R) of CD19+CD5+ and CD19+CD5− B cells; furthermore, we determined the IL-21 expression of iNKT cells as well. We also assessed the proportion of transitional (CD19+CD24highCD38high), mature (CD19+CD24intCD38int) and primarily memory (CD19+CD24highCD38-) B cells. CD5+ but not CD5− B cells showed elevated GrB and IL-21R expression in pSS; additionally IL-21 expression of iNKT cells was also elevated. The ratios of transitional and mature B cells were elevated in pSS, while primarily memory B cell percentages were decreased, which correlated with GrB and IL-21R expression of CD19+ B cells. Our results suggest that enhanced IL-21R expression of CD19+CD5+ B cells and production of IL-21 by iNKT cells may play an important role in the pathogenesis of pSS by regulating CD19+CD5+ B cell functions and increasing GrB production, presumably leading to a counter-regulatory effect in the disease.http://dx.doi.org/10.1155/2016/4328372 |
| spellingShingle | Gábor Papp Edit Gyimesi Krisztina Szabó Éva Zöld Margit Zeher Increased IL-21 Expression Induces Granzyme B in Peripheral CD5+ B Cells as a Potential Counter-Regulatory Effect in Primary Sjögren’s Syndrome Mediators of Inflammation |
| title | Increased IL-21 Expression Induces Granzyme B in Peripheral CD5+ B Cells as a Potential Counter-Regulatory Effect in Primary Sjögren’s Syndrome |
| title_full | Increased IL-21 Expression Induces Granzyme B in Peripheral CD5+ B Cells as a Potential Counter-Regulatory Effect in Primary Sjögren’s Syndrome |
| title_fullStr | Increased IL-21 Expression Induces Granzyme B in Peripheral CD5+ B Cells as a Potential Counter-Regulatory Effect in Primary Sjögren’s Syndrome |
| title_full_unstemmed | Increased IL-21 Expression Induces Granzyme B in Peripheral CD5+ B Cells as a Potential Counter-Regulatory Effect in Primary Sjögren’s Syndrome |
| title_short | Increased IL-21 Expression Induces Granzyme B in Peripheral CD5+ B Cells as a Potential Counter-Regulatory Effect in Primary Sjögren’s Syndrome |
| title_sort | increased il 21 expression induces granzyme b in peripheral cd5 b cells as a potential counter regulatory effect in primary sjogren s syndrome |
| url | http://dx.doi.org/10.1155/2016/4328372 |
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