Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection
Human leukocyte antigen-G (HLA-G) is a nonclassical HLA class I antigen that is expressed during pregnancy contributing to maternal-fetal tolerance. HLA-G can be expressed as membrane-bound and soluble forms. HLA-G expression increases strongly during viral infections such as congenital human cytome...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/3890306 |
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| author | Roberta Rizzo Liliana Gabrielli Daria Bortolotti Valentina Gentili Giulia Piccirilli Angela Chiereghin Claudia Pavia Silvia Bolzani Brunella Guerra Giuliana Simonazzi Francesca Cervi Maria Grazia Capretti Enrico Fainardi Dario Di Luca Maria Paola Landini Tiziana Lazzarotto |
| author_facet | Roberta Rizzo Liliana Gabrielli Daria Bortolotti Valentina Gentili Giulia Piccirilli Angela Chiereghin Claudia Pavia Silvia Bolzani Brunella Guerra Giuliana Simonazzi Francesca Cervi Maria Grazia Capretti Enrico Fainardi Dario Di Luca Maria Paola Landini Tiziana Lazzarotto |
| author_sort | Roberta Rizzo |
| collection | DOAJ |
| description | Human leukocyte antigen-G (HLA-G) is a nonclassical HLA class I antigen that is expressed during pregnancy contributing to maternal-fetal tolerance. HLA-G can be expressed as membrane-bound and soluble forms. HLA-G expression increases strongly during viral infections such as congenital human cytomegalovirus (HCMV) infections, with functional consequences in immunoregulation. In this work we investigated the expression of soluble (s)HLA-G and beta-2 microglobulin (component of HLA) molecules in correlation with the risk of transmission and severity of congenital HCMV infection. We analyzed 182 blood samples from 130 pregnant women and 52 nonpregnant women and 56 amniotic fluid samples from women experiencing primary HCMV infection. The median levels of sHLA-G in maternal serum of women with primary HCMV infection were higher in comparison with nonprimary and uninfected pregnant women (p<0.001). AF from HCMV symptomatic fetuses presented higher sHLA-G levels in comparison with infected asymptomatic fetuses (p<0.001), presence of HLA-G free-heavy chain, and a concentration gradient from amniotic fluid to maternal blood. No significant statistical difference of beta-2 microglobulin median levels was observed between all different groups. Our results suggest the determination of sHLA-G molecules in both maternal blood and amniotic fluid as a promising biomarker of diagnosis of maternal HCMV primary infection and fetal HCMV disease. |
| format | Article |
| id | doaj-art-4758fedd0b4a423793d815f442a75ca5 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-4758fedd0b4a423793d815f442a75ca52025-02-03T05:59:48ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/38903063890306Study of Soluble HLA-G in Congenital Human Cytomegalovirus InfectionRoberta Rizzo0Liliana Gabrielli1Daria Bortolotti2Valentina Gentili3Giulia Piccirilli4Angela Chiereghin5Claudia Pavia6Silvia Bolzani7Brunella Guerra8Giuliana Simonazzi9Francesca Cervi10Maria Grazia Capretti11Enrico Fainardi12Dario Di Luca13Maria Paola Landini14Tiziana Lazzarotto15Department of Medical Sciences, Section of Microbiology and Medical Genetics, University of Ferrara, Ferrara, ItalyOperative Unit of Clinical Microbiology, St. Orsola-Malpighi University Hospital, Bologna, ItalyDepartment of Medical Sciences, Section of Microbiology and Medical Genetics, University of Ferrara, Ferrara, ItalyDepartment of Medical Sciences, Section of Microbiology and Medical Genetics, University of Ferrara, Ferrara, ItalyOperative Unit of Clinical Microbiology, St. Orsola-Malpighi University Hospital, Bologna, ItalyOperative Unit of Clinical Microbiology, St. Orsola-Malpighi University Hospital, Bologna, ItalyOperative Unit of Clinical Microbiology, St. Orsola-Malpighi University Hospital, Bologna, ItalyDepartment of Medical Sciences, Section of Microbiology and Medical Genetics, University of Ferrara, Ferrara, ItalyOperative Unit of Obstetrics and Prenatal Medicine, Department of Medical and Surgical Sciences, St. Orsola-Malpighi University Hospital, University of Bologna, Bologna, ItalyOperative Unit of Obstetrics and Prenatal Medicine, Department of Medical and Surgical Sciences, St. Orsola-Malpighi University Hospital, University of Bologna, Bologna, ItalyOperative Unit of Obstetrics and Prenatal Medicine, Department of Medical and Surgical Sciences, St. Orsola-Malpighi University Hospital, University of Bologna, Bologna, ItalyOperative Unit of Neonatology, St. Orsola-Malpighi University Hospital, Bologna, ItalyOperative Unit of Neuroradiology, Careggi University Hospital, Firenze, ItalyDepartment of Medical Sciences, Section of Microbiology and Medical Genetics, University of Ferrara, Ferrara, ItalyOperative Unit of Clinical Microbiology, Department of Specialised, Experimental, and Diagnostic Medicine, St. Orsola-Malpighi University Hospital, University of Bologna, Bologna, ItalyOperative Unit of Clinical Microbiology, Department of Specialised, Experimental, and Diagnostic Medicine, St. Orsola-Malpighi University Hospital, University of Bologna, Bologna, ItalyHuman leukocyte antigen-G (HLA-G) is a nonclassical HLA class I antigen that is expressed during pregnancy contributing to maternal-fetal tolerance. HLA-G can be expressed as membrane-bound and soluble forms. HLA-G expression increases strongly during viral infections such as congenital human cytomegalovirus (HCMV) infections, with functional consequences in immunoregulation. In this work we investigated the expression of soluble (s)HLA-G and beta-2 microglobulin (component of HLA) molecules in correlation with the risk of transmission and severity of congenital HCMV infection. We analyzed 182 blood samples from 130 pregnant women and 52 nonpregnant women and 56 amniotic fluid samples from women experiencing primary HCMV infection. The median levels of sHLA-G in maternal serum of women with primary HCMV infection were higher in comparison with nonprimary and uninfected pregnant women (p<0.001). AF from HCMV symptomatic fetuses presented higher sHLA-G levels in comparison with infected asymptomatic fetuses (p<0.001), presence of HLA-G free-heavy chain, and a concentration gradient from amniotic fluid to maternal blood. No significant statistical difference of beta-2 microglobulin median levels was observed between all different groups. Our results suggest the determination of sHLA-G molecules in both maternal blood and amniotic fluid as a promising biomarker of diagnosis of maternal HCMV primary infection and fetal HCMV disease.http://dx.doi.org/10.1155/2016/3890306 |
| spellingShingle | Roberta Rizzo Liliana Gabrielli Daria Bortolotti Valentina Gentili Giulia Piccirilli Angela Chiereghin Claudia Pavia Silvia Bolzani Brunella Guerra Giuliana Simonazzi Francesca Cervi Maria Grazia Capretti Enrico Fainardi Dario Di Luca Maria Paola Landini Tiziana Lazzarotto Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection Journal of Immunology Research |
| title | Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection |
| title_full | Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection |
| title_fullStr | Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection |
| title_full_unstemmed | Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection |
| title_short | Study of Soluble HLA-G in Congenital Human Cytomegalovirus Infection |
| title_sort | study of soluble hla g in congenital human cytomegalovirus infection |
| url | http://dx.doi.org/10.1155/2016/3890306 |
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