NLRP3 is a BMI-independent mediator of stable COPD
Abstract Purpose The inflammatory response in animal models of chronic obstructive pulmonary disease (COPD) is activated by the NLR-family-pyrin-domain-containing-3 (NLRP3) inflammasome pathway, which is also known to play a role in obesity-related inflammation. The NLRP3/caspase-1/interleukin (IL)-...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | BMC Pulmonary Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12890-024-03435-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832586051714023424 |
|---|---|
| author | Yonca Gungor Selin Ercan Saliha Selin Özuygur Ermiş Yiğit Kozalı Gizem Kursunluoglu Ceyda Sahan Aylin Ozgen Alpaydin Hulya Ayar Kayali |
| author_facet | Yonca Gungor Selin Ercan Saliha Selin Özuygur Ermiş Yiğit Kozalı Gizem Kursunluoglu Ceyda Sahan Aylin Ozgen Alpaydin Hulya Ayar Kayali |
| author_sort | Yonca Gungor |
| collection | DOAJ |
| description | Abstract Purpose The inflammatory response in animal models of chronic obstructive pulmonary disease (COPD) is activated by the NLR-family-pyrin-domain-containing-3 (NLRP3) inflammasome pathway, which is also known to play a role in obesity-related inflammation. The NLRP3/caspase-1/interleukin (IL)-1β pathway might be involved in the progression of COPD with increasing body mass index. To our knowledge, no previous studies have explored the role of NLRP3 inflammasome markers in linking COPD and obesity. Here, we aim to investigate this potential connection by examining levels of NLRP3, caspase-1, IL-1β, and IL-17A and to provide additional data on the expression of these molecules in relation to smoking status and COPD severity. Methods A case‒control study was conducted between July 2020 and March 2023. Peripheral blood mononuclear cells were isolated, and total RNA was extracted for real-time quantitative polymerase chain reaction (qPCR) analysis to measure the expression levels of inflammasome molecules. Results 29 subjects who were diagnosed with stable COPD and 32 controls were included in the data analysis. NLRP3 and IL-17A but not caspase-1 or IL-1β expression was significantly greater in the COPD group than in the control group. We detected a significant increase in NLRP3 levels in the smoker COPD group (p = 0.009) and nonsmoker COPD group (p = 0.045) compared with those in the nonsmoker control group. There was no significant correlation between BMI and the inflammasome markers. Conclusion As proinflammatory biomarkers, NLRP3 and IL-17A are prominent in stable COPD patients. Smoking may trigger NLRP3-mediated inflammation in stable COPD patients. The expression levels of NLRP3 inflammasome molecules did not differ in terms of disease severity or BMI. Graphical Abstract |
| format | Article |
| id | doaj-art-472060a8e36d4028a804c6efcd2b3fcd |
| institution | Kabale University |
| issn | 1471-2466 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pulmonary Medicine |
| spelling | doaj-art-472060a8e36d4028a804c6efcd2b3fcd2025-01-26T12:13:01ZengBMCBMC Pulmonary Medicine1471-24662025-01-0125111010.1186/s12890-024-03435-6NLRP3 is a BMI-independent mediator of stable COPDYonca Gungor0Selin Ercan1Saliha Selin Özuygur Ermiş2Yiğit Kozalı3Gizem Kursunluoglu4Ceyda Sahan5Aylin Ozgen Alpaydin6Hulya Ayar Kayali7İzmir International Biomedicine and Genome Institute, Dokuz Eylül UniversityKrefting Research Centre, Institute of Medicine, Sahlgrenska Academy, University of GothenburgKrefting Research Centre, Institute of Medicine, Sahlgrenska Academy, University of Gothenburgİzmir Biomedicine and Genome CenterERFARMA Drug Application and Research Center, Erciyes UniversityDepartment of Public Health, Dokuz Eylul UniversityDepartment of Chest Diseases, Faculty of Medicine, Dokuz Eylül Universityİzmir International Biomedicine and Genome Institute, Dokuz Eylül UniversityAbstract Purpose The inflammatory response in animal models of chronic obstructive pulmonary disease (COPD) is activated by the NLR-family-pyrin-domain-containing-3 (NLRP3) inflammasome pathway, which is also known to play a role in obesity-related inflammation. The NLRP3/caspase-1/interleukin (IL)-1β pathway might be involved in the progression of COPD with increasing body mass index. To our knowledge, no previous studies have explored the role of NLRP3 inflammasome markers in linking COPD and obesity. Here, we aim to investigate this potential connection by examining levels of NLRP3, caspase-1, IL-1β, and IL-17A and to provide additional data on the expression of these molecules in relation to smoking status and COPD severity. Methods A case‒control study was conducted between July 2020 and March 2023. Peripheral blood mononuclear cells were isolated, and total RNA was extracted for real-time quantitative polymerase chain reaction (qPCR) analysis to measure the expression levels of inflammasome molecules. Results 29 subjects who were diagnosed with stable COPD and 32 controls were included in the data analysis. NLRP3 and IL-17A but not caspase-1 or IL-1β expression was significantly greater in the COPD group than in the control group. We detected a significant increase in NLRP3 levels in the smoker COPD group (p = 0.009) and nonsmoker COPD group (p = 0.045) compared with those in the nonsmoker control group. There was no significant correlation between BMI and the inflammasome markers. Conclusion As proinflammatory biomarkers, NLRP3 and IL-17A are prominent in stable COPD patients. Smoking may trigger NLRP3-mediated inflammation in stable COPD patients. The expression levels of NLRP3 inflammasome molecules did not differ in terms of disease severity or BMI. Graphical Abstracthttps://doi.org/10.1186/s12890-024-03435-6COPDSmokingInflammasomeNLRP3IL-17AObesity |
| spellingShingle | Yonca Gungor Selin Ercan Saliha Selin Özuygur Ermiş Yiğit Kozalı Gizem Kursunluoglu Ceyda Sahan Aylin Ozgen Alpaydin Hulya Ayar Kayali NLRP3 is a BMI-independent mediator of stable COPD BMC Pulmonary Medicine COPD Smoking Inflammasome NLRP3 IL-17A Obesity |
| title | NLRP3 is a BMI-independent mediator of stable COPD |
| title_full | NLRP3 is a BMI-independent mediator of stable COPD |
| title_fullStr | NLRP3 is a BMI-independent mediator of stable COPD |
| title_full_unstemmed | NLRP3 is a BMI-independent mediator of stable COPD |
| title_short | NLRP3 is a BMI-independent mediator of stable COPD |
| title_sort | nlrp3 is a bmi independent mediator of stable copd |
| topic | COPD Smoking Inflammasome NLRP3 IL-17A Obesity |
| url | https://doi.org/10.1186/s12890-024-03435-6 |
| work_keys_str_mv | AT yoncagungor nlrp3isabmiindependentmediatorofstablecopd AT selinercan nlrp3isabmiindependentmediatorofstablecopd AT salihaselinozuygurermis nlrp3isabmiindependentmediatorofstablecopd AT yigitkozalı nlrp3isabmiindependentmediatorofstablecopd AT gizemkursunluoglu nlrp3isabmiindependentmediatorofstablecopd AT ceydasahan nlrp3isabmiindependentmediatorofstablecopd AT aylinozgenalpaydin nlrp3isabmiindependentmediatorofstablecopd AT hulyaayarkayali nlrp3isabmiindependentmediatorofstablecopd |