Antibacterial activity of Streptomyces spp. crude extract on multidrug resistant Psedomonas aeruginosa isolated from clinical samples
Abstract Background: Actinomycetes are the heterogeneous group of microorganisms, which have thread-like filaments, isolated from the soil. Objectives: Isolation, identification, and partial purification of crude bioactive agents of the Actinomycetes isolates and isolation and identification of Pseu...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Medical Journal of Babylon |
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| Online Access: | https://doi.org/10.4103/MJBL.MJBL_350_22 |
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| author | Zainab Hasan Abed Eman Mohammad Jarallah |
| author_facet | Zainab Hasan Abed Eman Mohammad Jarallah |
| author_sort | Zainab Hasan Abed |
| collection | DOAJ |
| description | Abstract Background: Actinomycetes are the heterogeneous group of microorganisms, which have thread-like filaments, isolated from the soil. Objectives: Isolation, identification, and partial purification of crude bioactive agents of the Actinomycetes isolates and isolation and identification of Pseudomonas aeruginosa from clinical samples, and to study antibiotics susceptibility for these isolates. Materials and Methods: Forty soil samples were collected from different agriculture locations in Hilla city. Soil samples were air dried and pre-treated with calcium carbonate (1%). The best selective medium for growth Actinomycetes is yeast malt extract agar medium (International Streptomyces project—ISP2) with 1 mg/mL Nystatin as antifungal agent. Results: A total of 68 actinomycetes species were isolated and 23 Streptomyces spp. isolates were recovered after molecular identification. By cross- streaking method , the antibacterial activity for these isolated were tested against 30 clinical isolates of P. aeruginosa that showed resistance to 17 antibiotic types of streptomyces isolates demonstrated potential antibacterial activity were mass cultured and secondary metabolites were extracted using ethyl acetate. Among the 23 isolated strains, seven isolates (St4, St5, St6, St8, St11, St12, and St14) demonstrated a significant antibacterial activity with the zone of inhibition ranging from 0 to 22 mm against P. aeruginosa, according to the primary and secondary screening. Conclusion: The isolates St6 and St12 showed the higher antimicrobial metabolites activity, so these isolates were elected for sequencing. |
| format | Article |
| id | doaj-art-4709667bc9c444b98c86e50c0b63cc17 |
| institution | Kabale University |
| issn | 1812-156X 2312-6760 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Babylon |
| spelling | doaj-art-4709667bc9c444b98c86e50c0b63cc172025-01-25T10:14:50ZengWolters Kluwer Medknow PublicationsMedical Journal of Babylon1812-156X2312-67602024-01-01211152410.4103/MJBL.MJBL_350_22Antibacterial activity of Streptomyces spp. crude extract on multidrug resistant Psedomonas aeruginosa isolated from clinical samplesZainab Hasan AbedEman Mohammad JarallahAbstract Background: Actinomycetes are the heterogeneous group of microorganisms, which have thread-like filaments, isolated from the soil. Objectives: Isolation, identification, and partial purification of crude bioactive agents of the Actinomycetes isolates and isolation and identification of Pseudomonas aeruginosa from clinical samples, and to study antibiotics susceptibility for these isolates. Materials and Methods: Forty soil samples were collected from different agriculture locations in Hilla city. Soil samples were air dried and pre-treated with calcium carbonate (1%). The best selective medium for growth Actinomycetes is yeast malt extract agar medium (International Streptomyces project—ISP2) with 1 mg/mL Nystatin as antifungal agent. Results: A total of 68 actinomycetes species were isolated and 23 Streptomyces spp. isolates were recovered after molecular identification. By cross- streaking method , the antibacterial activity for these isolated were tested against 30 clinical isolates of P. aeruginosa that showed resistance to 17 antibiotic types of streptomyces isolates demonstrated potential antibacterial activity were mass cultured and secondary metabolites were extracted using ethyl acetate. Among the 23 isolated strains, seven isolates (St4, St5, St6, St8, St11, St12, and St14) demonstrated a significant antibacterial activity with the zone of inhibition ranging from 0 to 22 mm against P. aeruginosa, according to the primary and secondary screening. Conclusion: The isolates St6 and St12 showed the higher antimicrobial metabolites activity, so these isolates were elected for sequencing.https://doi.org/10.4103/MJBL.MJBL_350_22actinomycetesantibioticmetabolismpseudomonas aeruginosa |
| spellingShingle | Zainab Hasan Abed Eman Mohammad Jarallah Antibacterial activity of Streptomyces spp. crude extract on multidrug resistant Psedomonas aeruginosa isolated from clinical samples Medical Journal of Babylon actinomycetes antibiotic metabolism pseudomonas aeruginosa |
| title | Antibacterial activity of Streptomyces spp. crude extract on multidrug resistant Psedomonas aeruginosa isolated from clinical samples |
| title_full | Antibacterial activity of Streptomyces spp. crude extract on multidrug resistant Psedomonas aeruginosa isolated from clinical samples |
| title_fullStr | Antibacterial activity of Streptomyces spp. crude extract on multidrug resistant Psedomonas aeruginosa isolated from clinical samples |
| title_full_unstemmed | Antibacterial activity of Streptomyces spp. crude extract on multidrug resistant Psedomonas aeruginosa isolated from clinical samples |
| title_short | Antibacterial activity of Streptomyces spp. crude extract on multidrug resistant Psedomonas aeruginosa isolated from clinical samples |
| title_sort | antibacterial activity of streptomyces spp crude extract on multidrug resistant psedomonas aeruginosa isolated from clinical samples |
| topic | actinomycetes antibiotic metabolism pseudomonas aeruginosa |
| url | https://doi.org/10.4103/MJBL.MJBL_350_22 |
| work_keys_str_mv | AT zainabhasanabed antibacterialactivityofstreptomycessppcrudeextractonmultidrugresistantpsedomonasaeruginosaisolatedfromclinicalsamples AT emanmohammadjarallah antibacterialactivityofstreptomycessppcrudeextractonmultidrugresistantpsedomonasaeruginosaisolatedfromclinicalsamples |