A Microfluidic Biosensor for Quantitative Detection of <i>Salmonella</i> in Traditional Chinese Medicine
Microbial contamination is an important factor threatening the safety of Chinese medicine preparations, and microfluidic detection methods have demonstrated excellent advantages in the application of rapid bacterial detection. In our study, a novel optical biosensor was developed for the rapid and s...
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2024-12-01
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| Series: | Biosensors |
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| author | Yutong Wu Yang Liu Jinchen Ma Shanxi Zhu Xiaojun Zhao Huawei Mou Xuanqi Ke Zhisheng Wu Yifei Wang Sheng Lin Wuzhen Qi |
| author_facet | Yutong Wu Yang Liu Jinchen Ma Shanxi Zhu Xiaojun Zhao Huawei Mou Xuanqi Ke Zhisheng Wu Yifei Wang Sheng Lin Wuzhen Qi |
| author_sort | Yutong Wu |
| collection | DOAJ |
| description | Microbial contamination is an important factor threatening the safety of Chinese medicine preparations, and microfluidic detection methods have demonstrated excellent advantages in the application of rapid bacterial detection. In our study, a novel optical biosensor was developed for the rapid and sensitive detection of <i>Salmonella</i> in traditional Chinese medicine on a microfluidic chip. Immune gold@platinum nanocatalysts (Au@PtNCs) were utilized for specific bacterial labeling, while magnetic nano-beads (MNBs) with a novel high-gradient magnetic field were employed for the specific capture of bacteria. The immune MNBs, immune Au@PtNCs, and bacterial samples were introduced into a novel passive microfluidic micromixer for full mixing, resulting in the formation of a double-antibody sandwich structure due to antigen–antibody immune reactions. Subsequently, the mixture flowed into the reaction cell, where the MNBs-<i>Salmonella</i>-Au@PtNCs complex was captured by the magnetic field. After washing, hydrogen peroxide-tetramethylbenzidine substrate (H<sub>2</sub>O<sub>2</sub>-TMB) was added, reacting with the Au@PtNCs peroxidase to produce a blue reaction product. This entire process was automated using a portable device, and <i>Salmonella</i> concentration was analyzed via a phone application. This simple biosensor has good specificity with a detection range of 9 × 10<sup>1</sup>–9 × 10<sup>5</sup> CFU/mL and can detect <i>Salmonella</i> concentrations as low as 90 CFU/mL within 74 min. The average recoveries of the spiked samples ranged from 76.8% to 109.5% |
| format | Article |
| id | doaj-art-46fc95eaa9194af99f491dae30f23f64 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-46fc95eaa9194af99f491dae30f23f642025-01-24T13:25:25ZengMDPI AGBiosensors2079-63742024-12-011511010.3390/bios15010010A Microfluidic Biosensor for Quantitative Detection of <i>Salmonella</i> in Traditional Chinese MedicineYutong Wu0Yang Liu1Jinchen Ma2Shanxi Zhu3Xiaojun Zhao4Huawei Mou5Xuanqi Ke6Zhisheng Wu7Yifei Wang8Sheng Lin9Wuzhen Qi10School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaChina Center for Information Industry Development, Beijing 100036, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaFangshan Hospital of Beijing University of Chinese Medicine, Beijing 102400, ChinaDongzhimen Hospital of Beijing University of Chinese Medicine, Beijing 100700, ChinaSchool of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, ChinaMicrobial contamination is an important factor threatening the safety of Chinese medicine preparations, and microfluidic detection methods have demonstrated excellent advantages in the application of rapid bacterial detection. In our study, a novel optical biosensor was developed for the rapid and sensitive detection of <i>Salmonella</i> in traditional Chinese medicine on a microfluidic chip. Immune gold@platinum nanocatalysts (Au@PtNCs) were utilized for specific bacterial labeling, while magnetic nano-beads (MNBs) with a novel high-gradient magnetic field were employed for the specific capture of bacteria. The immune MNBs, immune Au@PtNCs, and bacterial samples were introduced into a novel passive microfluidic micromixer for full mixing, resulting in the formation of a double-antibody sandwich structure due to antigen–antibody immune reactions. Subsequently, the mixture flowed into the reaction cell, where the MNBs-<i>Salmonella</i>-Au@PtNCs complex was captured by the magnetic field. After washing, hydrogen peroxide-tetramethylbenzidine substrate (H<sub>2</sub>O<sub>2</sub>-TMB) was added, reacting with the Au@PtNCs peroxidase to produce a blue reaction product. This entire process was automated using a portable device, and <i>Salmonella</i> concentration was analyzed via a phone application. This simple biosensor has good specificity with a detection range of 9 × 10<sup>1</sup>–9 × 10<sup>5</sup> CFU/mL and can detect <i>Salmonella</i> concentrations as low as 90 CFU/mL within 74 min. The average recoveries of the spiked samples ranged from 76.8% to 109.5%https://www.mdpi.com/2079-6374/15/1/10microfluidicsbiosensornanocatalystsmicrobial contaminationtraditional Chinese medicine |
| spellingShingle | Yutong Wu Yang Liu Jinchen Ma Shanxi Zhu Xiaojun Zhao Huawei Mou Xuanqi Ke Zhisheng Wu Yifei Wang Sheng Lin Wuzhen Qi A Microfluidic Biosensor for Quantitative Detection of <i>Salmonella</i> in Traditional Chinese Medicine Biosensors microfluidics biosensor nanocatalysts microbial contamination traditional Chinese medicine |
| title | A Microfluidic Biosensor for Quantitative Detection of <i>Salmonella</i> in Traditional Chinese Medicine |
| title_full | A Microfluidic Biosensor for Quantitative Detection of <i>Salmonella</i> in Traditional Chinese Medicine |
| title_fullStr | A Microfluidic Biosensor for Quantitative Detection of <i>Salmonella</i> in Traditional Chinese Medicine |
| title_full_unstemmed | A Microfluidic Biosensor for Quantitative Detection of <i>Salmonella</i> in Traditional Chinese Medicine |
| title_short | A Microfluidic Biosensor for Quantitative Detection of <i>Salmonella</i> in Traditional Chinese Medicine |
| title_sort | microfluidic biosensor for quantitative detection of i salmonella i in traditional chinese medicine |
| topic | microfluidics biosensor nanocatalysts microbial contamination traditional Chinese medicine |
| url | https://www.mdpi.com/2079-6374/15/1/10 |
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