Testing of Ferarabivac anti-rabies live vaccine for wild carnivores for its immunogenicity and protectivity
Rabies is one of the most important human and animal viral diseases, being one of the most dangerous zoonoses, causing damage to the central nervous system with an inevitable fatal outcome. This disease is of global concern, and it attracts special attention of international organizations (WHO, OIE,...
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Da Vinci Media
2020-03-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/455 |
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| author | A. V. Shishkov D. A. Lozovoy A. V. Borisov D. V. Mikhalishin |
| author_facet | A. V. Shishkov D. A. Lozovoy A. V. Borisov D. V. Mikhalishin |
| author_sort | A. V. Shishkov |
| collection | DOAJ |
| description | Rabies is one of the most important human and animal viral diseases, being one of the most dangerous zoonoses, causing damage to the central nervous system with an inevitable fatal outcome. This disease is of global concern, and it attracts special attention of international organizations (WHO, OIE, FAO, GARC) and of veterinary services in many countries around the world. A variety of anti-rabies vaccines have been used for specific rabies prevention in wild carnivores, however, the safety and effectiveness of some of them is doubtful. New, more advanced products are being developed, one of which is Ferarabivac, a live oral vaccine. The vaccine was tested for its immunogenicity and protectivity in wild carnivores. The optimal immunizing dose was 2.0 cm3, with the infectivity titre of RV-97 strain of at least 6.00 lg KKID50/cm3. Anti-rabies antibody titres detected in the blood sera of foxes and raccoon dogs 14 days post vaccination, were 0.70 ± 0.18 and 0.73 ± 0.19 IU/cm3, respectively, which provided protection against rabies virus infection (≥ 0.50 IU/cm3). Rabies virus neutralizing antibodies in foxes reached their maximum level of 4.30 ± 0.32 IU/cm3 50 days post vaccination. Antibody titres in vaccinated raccoon dogs also reached their maximum level of 4.53 ± 0.27 IU/cm3 50 days post vaccination. The minimum protective threshold levels of serum neutralizing antibodies was determined 12 months after the vaccination, and it was 0.62 ± 0.28 and 0.71 ± 0.17 IU/cm3 in foxes and raccoon dogs, respectively, which proves the necessity to perform booster vaccination one year later. No animals vaccinated against rabies with Ferarabivac live vaccine showed any clinical signs of the disease during the entire observation period following the challenge test carried out 30 days post vaccination. |
| format | Article |
| id | doaj-art-46d82b63714a4ab68f3e0004d844acfa |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Da Vinci Media |
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| series | Ветеринария сегодня |
| spelling | doaj-art-46d82b63714a4ab68f3e0004d844acfa2025-02-06T09:52:08ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592020-03-0101313710.29326/2304-196X-2020-1-32-31-37440Testing of Ferarabivac anti-rabies live vaccine for wild carnivores for its immunogenicity and protectivityA. V. Shishkov0D. A. Lozovoy1A. V. Borisov2D. V. Mikhalishin3FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)FGBI “Federal Centre for Animal Health” (FGBI “ARRIAH”)Rabies is one of the most important human and animal viral diseases, being one of the most dangerous zoonoses, causing damage to the central nervous system with an inevitable fatal outcome. This disease is of global concern, and it attracts special attention of international organizations (WHO, OIE, FAO, GARC) and of veterinary services in many countries around the world. A variety of anti-rabies vaccines have been used for specific rabies prevention in wild carnivores, however, the safety and effectiveness of some of them is doubtful. New, more advanced products are being developed, one of which is Ferarabivac, a live oral vaccine. The vaccine was tested for its immunogenicity and protectivity in wild carnivores. The optimal immunizing dose was 2.0 cm3, with the infectivity titre of RV-97 strain of at least 6.00 lg KKID50/cm3. Anti-rabies antibody titres detected in the blood sera of foxes and raccoon dogs 14 days post vaccination, were 0.70 ± 0.18 and 0.73 ± 0.19 IU/cm3, respectively, which provided protection against rabies virus infection (≥ 0.50 IU/cm3). Rabies virus neutralizing antibodies in foxes reached their maximum level of 4.30 ± 0.32 IU/cm3 50 days post vaccination. Antibody titres in vaccinated raccoon dogs also reached their maximum level of 4.53 ± 0.27 IU/cm3 50 days post vaccination. The minimum protective threshold levels of serum neutralizing antibodies was determined 12 months after the vaccination, and it was 0.62 ± 0.28 and 0.71 ± 0.17 IU/cm3 in foxes and raccoon dogs, respectively, which proves the necessity to perform booster vaccination one year later. No animals vaccinated against rabies with Ferarabivac live vaccine showed any clinical signs of the disease during the entire observation period following the challenge test carried out 30 days post vaccination.https://veterinary.arriah.ru/jour/article/view/455rabies of wild carnivorous animalsferarabivac anti-rabies live oral vaccinerabies virus neutralizing antibodies |
| spellingShingle | A. V. Shishkov D. A. Lozovoy A. V. Borisov D. V. Mikhalishin Testing of Ferarabivac anti-rabies live vaccine for wild carnivores for its immunogenicity and protectivity Ветеринария сегодня rabies of wild carnivorous animals ferarabivac anti-rabies live oral vaccine rabies virus neutralizing antibodies |
| title | Testing of Ferarabivac anti-rabies live vaccine for wild carnivores for its immunogenicity and protectivity |
| title_full | Testing of Ferarabivac anti-rabies live vaccine for wild carnivores for its immunogenicity and protectivity |
| title_fullStr | Testing of Ferarabivac anti-rabies live vaccine for wild carnivores for its immunogenicity and protectivity |
| title_full_unstemmed | Testing of Ferarabivac anti-rabies live vaccine for wild carnivores for its immunogenicity and protectivity |
| title_short | Testing of Ferarabivac anti-rabies live vaccine for wild carnivores for its immunogenicity and protectivity |
| title_sort | testing of ferarabivac anti rabies live vaccine for wild carnivores for its immunogenicity and protectivity |
| topic | rabies of wild carnivorous animals ferarabivac anti-rabies live oral vaccine rabies virus neutralizing antibodies |
| url | https://veterinary.arriah.ru/jour/article/view/455 |
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