Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to co...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/962538 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545300842020864 |
|---|---|
| author | Vicente P. Martins Carina S. Pinheiro Barbara C. P. Figueiredo Natan R. G. Assis Suellen B. Morais Marcelo V. Caliari Vasco Azevedo William Castro-Borges R. Alan Wilson Sergio C. Oliveira |
| author_facet | Vicente P. Martins Carina S. Pinheiro Barbara C. P. Figueiredo Natan R. G. Assis Suellen B. Morais Marcelo V. Caliari Vasco Azevedo William Castro-Borges R. Alan Wilson Sergio C. Oliveira |
| author_sort | Vicente P. Martins |
| collection | DOAJ |
| description | The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate. |
| format | Article |
| id | doaj-art-4661a143656e4106abf7722cdbd0a90f |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-4661a143656e4106abf7722cdbd0a90f2025-02-03T07:26:07ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/962538962538Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge InfectionVicente P. Martins0Carina S. Pinheiro1Barbara C. P. Figueiredo2Natan R. G. Assis3Suellen B. Morais4Marcelo V. Caliari5Vasco Azevedo6William Castro-Borges7R. Alan Wilson8Sergio C. Oliveira9Departamento de Bioquímica, Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica, Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica, Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica, Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica, Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Patologia Geral do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Biologia Geral do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartamento de Ciências Biológicas, Universidade Federal de Ouro Preto, 35400-000 Ouro Preto, MG, BrazilCentre for Immunology & Infection, Department of Biology, University of York, Heslington, York YO10 5DD, UKDepartamento de Bioquímica, Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilThe flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate.http://dx.doi.org/10.1155/2012/962538 |
| spellingShingle | Vicente P. Martins Carina S. Pinheiro Barbara C. P. Figueiredo Natan R. G. Assis Suellen B. Morais Marcelo V. Caliari Vasco Azevedo William Castro-Borges R. Alan Wilson Sergio C. Oliveira Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection Clinical and Developmental Immunology |
| title | Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection |
| title_full | Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection |
| title_fullStr | Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection |
| title_full_unstemmed | Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection |
| title_short | Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection |
| title_sort | vaccination with enzymatically cleaved gpi anchored proteins from schistosoma mansoni induces protection against challenge infection |
| url | http://dx.doi.org/10.1155/2012/962538 |
| work_keys_str_mv | AT vicentepmartins vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT carinaspinheiro vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT barbaracpfigueiredo vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT natanrgassis vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT suellenbmorais vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT marcelovcaliari vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT vascoazevedo vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT williamcastroborges vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT ralanwilson vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection AT sergiocoliveira vaccinationwithenzymaticallycleavedgpianchoredproteinsfromschistosomamansoniinducesprotectionagainstchallengeinfection |