Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc
Spermatogonial cells are capable of transmitting genetic information to the next generation and have the potential for self-renewal. However, research on spermatogonial cells is generally limited by the paucity of cell surface markers, which are not universal among species. In this study, we develop...
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Elsevier
2025-01-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2001037025000236 |
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| author | Liangjie Liu Ya Shu Tian Liu Huilan Wei Yaxin Yang Lijing Zhang Xiaohui Ma Guoqing Li Yajuan Li Shi Wang Lingling Zhang |
| author_facet | Liangjie Liu Ya Shu Tian Liu Huilan Wei Yaxin Yang Lijing Zhang Xiaohui Ma Guoqing Li Yajuan Li Shi Wang Lingling Zhang |
| author_sort | Liangjie Liu |
| collection | DOAJ |
| description | Spermatogonial cells are capable of transmitting genetic information to the next generation and have the potential for self-renewal. However, research on spermatogonial cells is generally limited by the paucity of cell surface markers, which are not universal among species. In this study, we developed a systematic screening strategy for germline regulatory pathways and spermatogonial surface markers in non-model organisms. This was achieved by combining weighted gene co-expression network analysis (WGCNA), differential expressed gene (DEG) overrepresentation analysis, and functional annotation. This strategy was employed to identify a spermatogonia-related module, which was found to be enriched with stem cell-related pathways in the scallop Patinopecten yessoensis. This module contained a transmembrane protein, fibroblast growth factor receptor (FGFR). A single copy of Fgfr (PyFgfr) was confirmed in the P. yessoensis genome, which exhibited the canonical functional domains of FGFRs. PyFgfr was universally expressed in multiple tissues, including the testis. The highest expression was observed at the resting stage of the testis, with exclusive localization in spermatogonia. To obtain antibodies that recognize the cell surface region, the extracellular domains of PyFGFR were used as an antigen to prepare antiserum. Western blotting, immunohistochemical, and immunofluorescent analyses demonstrated that the antiserum specifically detected PyFGFR in the spermatogonia. This study demonstrates the feasibility of this strategy for screening spermatogonial surface markers in the scallop. This approach will facilitate the culture and manipulation of spermatogonia in non-model organisms, which may contribute to genetic improvement in aquaculture. |
| format | Article |
| id | doaj-art-464b881489de41aaa92af501c43bd0e9 |
| institution | Kabale University |
| issn | 2001-0370 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj-art-464b881489de41aaa92af501c43bd0e92025-01-30T05:13:54ZengElsevierComputational and Structural Biotechnology Journal2001-03702025-01-0127519530Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve molluscLiangjie Liu0Ya Shu1Tian Liu2Huilan Wei3Yaxin Yang4Lijing Zhang5Xiaohui Ma6Guoqing Li7Yajuan Li8Shi Wang9Lingling Zhang10MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology & Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China; Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, ChinaMOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology & Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao 266237, China; Corresponding author at: MOE Key Laboratory of Marine Genetics and Breeding, Ocean University of China, Qingdao 266003, China.Spermatogonial cells are capable of transmitting genetic information to the next generation and have the potential for self-renewal. However, research on spermatogonial cells is generally limited by the paucity of cell surface markers, which are not universal among species. In this study, we developed a systematic screening strategy for germline regulatory pathways and spermatogonial surface markers in non-model organisms. This was achieved by combining weighted gene co-expression network analysis (WGCNA), differential expressed gene (DEG) overrepresentation analysis, and functional annotation. This strategy was employed to identify a spermatogonia-related module, which was found to be enriched with stem cell-related pathways in the scallop Patinopecten yessoensis. This module contained a transmembrane protein, fibroblast growth factor receptor (FGFR). A single copy of Fgfr (PyFgfr) was confirmed in the P. yessoensis genome, which exhibited the canonical functional domains of FGFRs. PyFgfr was universally expressed in multiple tissues, including the testis. The highest expression was observed at the resting stage of the testis, with exclusive localization in spermatogonia. To obtain antibodies that recognize the cell surface region, the extracellular domains of PyFGFR were used as an antigen to prepare antiserum. Western blotting, immunohistochemical, and immunofluorescent analyses demonstrated that the antiserum specifically detected PyFGFR in the spermatogonia. This study demonstrates the feasibility of this strategy for screening spermatogonial surface markers in the scallop. This approach will facilitate the culture and manipulation of spermatogonia in non-model organisms, which may contribute to genetic improvement in aquaculture.http://www.sciencedirect.com/science/article/pii/S2001037025000236FgfrSurface markerWGCNASpermatogonial cellsPatinopecten yessoensis |
| spellingShingle | Liangjie Liu Ya Shu Tian Liu Huilan Wei Yaxin Yang Lijing Zhang Xiaohui Ma Guoqing Li Yajuan Li Shi Wang Lingling Zhang Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc Computational and Structural Biotechnology Journal Fgfr Surface marker WGCNA Spermatogonial cells Patinopecten yessoensis |
| title | Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc |
| title_full | Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc |
| title_fullStr | Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc |
| title_full_unstemmed | Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc |
| title_short | Systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc |
| title_sort | systematic screening and dynamic profiling of germline regulatory pathways and spermatogonial surface markers in a bivalve mollusc |
| topic | Fgfr Surface marker WGCNA Spermatogonial cells Patinopecten yessoensis |
| url | http://www.sciencedirect.com/science/article/pii/S2001037025000236 |
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