Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis
Cross protection can undermine the effectiveness of control measures on foodborne pathogens, and therefore brings major implications for food safety. In this work, the capacity of Salmonella Enteritidis to mount ethanol tolerance following acid adaptation was characterized by analysis of cell viabil...
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| Language: | English |
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302200252X |
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| author | Shoukui He Beining Ye Zengfeng Zhang Yan Cui Siyun Wang Xianming Shi |
| author_facet | Shoukui He Beining Ye Zengfeng Zhang Yan Cui Siyun Wang Xianming Shi |
| author_sort | Shoukui He |
| collection | DOAJ |
| description | Cross protection can undermine the effectiveness of control measures on foodborne pathogens, and therefore brings major implications for food safety. In this work, the capacity of Salmonella Enteritidis to mount ethanol tolerance following acid adaptation was characterized by analysis of cell viability and cell membrane property. It was observed that preadaptation to pH 4.5 significantly (P < 0.05) increased the tolerance of log-phase cells to ethanol; in contrast, stationary-phase cells displayed reduced ethanol tolerance after acid adaptation. However, acid adaptation did not cause cell leakage and morphological change in both log-phase and stationary-phase S. Enteritidis. Fatty acid analysis further revealed that the amount of C14:0, C17:0 cyclo and C19:0 cyclo fatty acids was increased, while that of C16:1ω7c and C18:1ω7c fatty acids was decreased, respectively, in response to acid adaptation, regardless of bacterial growth phase. Notably, acid adaptation significantly (P < 0.05) increased the proportion of C16:0 fatty acid in log-phase cells, but this effect did not occur in stationary-phase cells. Moreover, exogenous addition of C16:0 fatty acid to stationary-phase acid-adapted cultures was able to enhance bacterial ethanol tolerance. Taken together, C16:0 fatty acid is involved in the growth-phase-dependent protective effect of acid adaptation on ethanol tolerance in S. Enteritidis. |
| format | Article |
| id | doaj-art-4647217d0ce2491da996f5a11802b557 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-4647217d0ce2491da996f5a11802b5572025-02-03T07:06:24ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112414021407Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella EnteritidisShoukui He0Beining Ye1Zengfeng Zhang2Yan Cui3Siyun Wang4Xianming Shi5MOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Lab of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Lab of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Lab of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaMOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Lab of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, ChinaFood, Nutrition and Health, Faculty of Land and Food Systems, The University of British Columbia, Vancouver BC V6T 1Z4, CanadaMOST-USDA Joint Research Center for Food Safety, School of Agriculture and Biology, State Key Lab of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China; Corresponding author at: Shanghai Jiao Tong University, Shanghai 200240, China. Fax: 86-21-3420-6616.Cross protection can undermine the effectiveness of control measures on foodborne pathogens, and therefore brings major implications for food safety. In this work, the capacity of Salmonella Enteritidis to mount ethanol tolerance following acid adaptation was characterized by analysis of cell viability and cell membrane property. It was observed that preadaptation to pH 4.5 significantly (P < 0.05) increased the tolerance of log-phase cells to ethanol; in contrast, stationary-phase cells displayed reduced ethanol tolerance after acid adaptation. However, acid adaptation did not cause cell leakage and morphological change in both log-phase and stationary-phase S. Enteritidis. Fatty acid analysis further revealed that the amount of C14:0, C17:0 cyclo and C19:0 cyclo fatty acids was increased, while that of C16:1ω7c and C18:1ω7c fatty acids was decreased, respectively, in response to acid adaptation, regardless of bacterial growth phase. Notably, acid adaptation significantly (P < 0.05) increased the proportion of C16:0 fatty acid in log-phase cells, but this effect did not occur in stationary-phase cells. Moreover, exogenous addition of C16:0 fatty acid to stationary-phase acid-adapted cultures was able to enhance bacterial ethanol tolerance. Taken together, C16:0 fatty acid is involved in the growth-phase-dependent protective effect of acid adaptation on ethanol tolerance in S. Enteritidis.http://www.sciencedirect.com/science/article/pii/S221345302200252XSalmonella EnteritidisAcid adaptationEthanol toleranceGrowth phaseFatty acid |
| spellingShingle | Shoukui He Beining Ye Zengfeng Zhang Yan Cui Siyun Wang Xianming Shi Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis Food Science and Human Wellness Salmonella Enteritidis Acid adaptation Ethanol tolerance Growth phase Fatty acid |
| title | Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis |
| title_full | Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis |
| title_fullStr | Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis |
| title_full_unstemmed | Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis |
| title_short | Cross-protective effect of acid adaptation on ethanol tolerance in Salmonella Enteritidis |
| title_sort | cross protective effect of acid adaptation on ethanol tolerance in salmonella enteritidis |
| topic | Salmonella Enteritidis Acid adaptation Ethanol tolerance Growth phase Fatty acid |
| url | http://www.sciencedirect.com/science/article/pii/S221345302200252X |
| work_keys_str_mv | AT shoukuihe crossprotectiveeffectofacidadaptationonethanoltoleranceinsalmonellaenteritidis AT beiningye crossprotectiveeffectofacidadaptationonethanoltoleranceinsalmonellaenteritidis AT zengfengzhang crossprotectiveeffectofacidadaptationonethanoltoleranceinsalmonellaenteritidis AT yancui crossprotectiveeffectofacidadaptationonethanoltoleranceinsalmonellaenteritidis AT siyunwang crossprotectiveeffectofacidadaptationonethanoltoleranceinsalmonellaenteritidis AT xianmingshi crossprotectiveeffectofacidadaptationonethanoltoleranceinsalmonellaenteritidis |