Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential
A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/727352 |
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| author | Robert Zajicek Vaclav Mandys Ondrej Mestak Jan Sevcik Radana Königova Eva Matouskova |
| author_facet | Robert Zajicek Vaclav Mandys Ondrej Mestak Jan Sevcik Radana Königova Eva Matouskova |
| author_sort | Robert Zajicek |
| collection | DOAJ |
| description | A number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important role in keratinocyte proliferation and formation of epidermal architecture in vitro as well as in vivo. The bioactivity of Xe-Derma was studied by a cell culture assay. We analyzed growth and differentiation of human keratinocytes cultured in vitro on Xe-Derma, and we compared the results with formation of neoepidermis in the deep dermal wounds treated with Xe-Derma. Keratinocytes cultured on Xe-Derma submerged in the culture medium achieved confluence in 7–10 days. After lifting the cultures to the air-liquid interface, the keratinocytes were stratified and differentiated within one week, forming an epidermis with basal, spinous, granular, and stratum corneum layers. Immunohistochemical detection of high-molecular weight cytokeratins (HMW CKs), CD29, p63, and involucrin confirmed the similarity of organization and differentiation of the cultured epidermal cells to the normal epidermis. The results suggest that the firm natural structure of Xe-Derma stimulates proliferation and differentiation of human primary keratinocytes and by this way improves wound healing. |
| format | Article |
| id | doaj-art-4643d5ac38d64059a6ac9aba28076faf |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4643d5ac38d64059a6ac9aba28076faf2025-02-03T06:04:55ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/727352727352Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing PotentialRobert Zajicek0Vaclav Mandys1Ondrej Mestak2Jan Sevcik3Radana Königova4Eva Matouskova5Prague Burn Centre, 3rd Faculty of Medicine, Charles University in Prague, 100 00 Prague 10, Czech RepublicDepartment of Pathology, 3rd Faculty of Medicine, Charles University in Prague, 100 00 Prague 10, Czech RepublicDepartment of Plastic Surgery, 1st Faculty of Medicine, Charles University in Prague, 180 00 Prague 2, Czech RepublicPrague Burn Centre, 3rd Faculty of Medicine, Charles University in Prague, 100 00 Prague 10, Czech RepublicPrague Burn Centre, 3rd Faculty of Medicine, Charles University in Prague, 100 00 Prague 10, Czech RepublicPrague Burn Centre, 3rd Faculty of Medicine, Charles University in Prague, 100 00 Prague 10, Czech RepublicA number of implantable biomaterials derived from animal tissues are now used in modern surgery. Xe-Derma is a dry, sterile, acellular porcine dermis. It has a remarkable healing effect on burns and other wounds. Our hypothesis was that the natural biological structure of Xe-Derma plays an important role in keratinocyte proliferation and formation of epidermal architecture in vitro as well as in vivo. The bioactivity of Xe-Derma was studied by a cell culture assay. We analyzed growth and differentiation of human keratinocytes cultured in vitro on Xe-Derma, and we compared the results with formation of neoepidermis in the deep dermal wounds treated with Xe-Derma. Keratinocytes cultured on Xe-Derma submerged in the culture medium achieved confluence in 7–10 days. After lifting the cultures to the air-liquid interface, the keratinocytes were stratified and differentiated within one week, forming an epidermis with basal, spinous, granular, and stratum corneum layers. Immunohistochemical detection of high-molecular weight cytokeratins (HMW CKs), CD29, p63, and involucrin confirmed the similarity of organization and differentiation of the cultured epidermal cells to the normal epidermis. The results suggest that the firm natural structure of Xe-Derma stimulates proliferation and differentiation of human primary keratinocytes and by this way improves wound healing.http://dx.doi.org/10.1100/2012/727352 |
| spellingShingle | Robert Zajicek Vaclav Mandys Ondrej Mestak Jan Sevcik Radana Königova Eva Matouskova Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential The Scientific World Journal |
| title | Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential |
| title_full | Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential |
| title_fullStr | Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential |
| title_full_unstemmed | Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential |
| title_short | Human Keratinocyte Growth and Differentiation on Acellular Porcine Dermal Matrix in relation to Wound Healing Potential |
| title_sort | human keratinocyte growth and differentiation on acellular porcine dermal matrix in relation to wound healing potential |
| url | http://dx.doi.org/10.1100/2012/727352 |
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