Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment
Atherosclerosis is an inflammatory disease characterized by immunological activity, in which endothelial dysfunction represents an early event leading to subsequent inflammatory vascular damage. We investigated gene expression of the adhesion molecules (AMs) ICAM-1, VCAM-1, and β1-integrin in endoth...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Vascular Medicine |
| Online Access: | http://dx.doi.org/10.1155/2013/412761 |
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| author | Franca Marino Luigina Guasti Matteo Tozzi Laura Schembri Luana Castiglioni Elisabetta Molteni Gabriele Piffaretti Patrizio Castelli Marco Cosentino |
| author_facet | Franca Marino Luigina Guasti Matteo Tozzi Laura Schembri Luana Castiglioni Elisabetta Molteni Gabriele Piffaretti Patrizio Castelli Marco Cosentino |
| author_sort | Franca Marino |
| collection | DOAJ |
| description | Atherosclerosis is an inflammatory disease characterized by immunological activity, in which endothelial dysfunction represents an early event leading to subsequent inflammatory vascular damage. We investigated gene expression of the adhesion molecules (AMs) ICAM-1, VCAM-1, and β1-integrin in endothelial cells (ECs) isolated from venous blood (circulating EC, cEC) and purified from femoral plaques (pEC) obtained from 9 patients with peripheral artery disease (PAD) submitted to femoral artery thrombendarterectomy (FEA). In addition, in peripheral blood mononuclear cells (PBMCs) of the same subjects, we investigated gene expression of IFN-γ, IL-4, TGF-β, and IL-10. Patients were longitudinally evaluated 1 month before surgery, when statin treatment was established, at the time of surgery, and after 2 and 5 months. All AM mRNA levels, measured by means of real-time PCR, in cEC diminished during the study, up to 41–50% of initial levels at followup. AM mRNA expression was significantly higher in pEC than in cEC. During the study, in PBMCs, TGF-β and IL-10 mRNA levels remained unchanged while IFN-γ and IL-4 levels increased; however, the ratio IFN-γ/IL-4 showed no significant modification. In PAD patients, FEA and statin treatment induce a profound reduction of AM expression in cEC and affect cytokine mRNA expression in PBMCs. |
| format | Article |
| id | doaj-art-4631df48b3a34b7d97f6844a26d2b1ee |
| institution | Kabale University |
| issn | 2090-2824 2090-2832 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Vascular Medicine |
| spelling | doaj-art-4631df48b3a34b7d97f6844a26d2b1ee2025-02-03T01:01:43ZengWileyInternational Journal of Vascular Medicine2090-28242090-28322013-01-01201310.1155/2013/412761412761Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological TreatmentFranca Marino0Luigina Guasti1Matteo Tozzi2Laura Schembri3Luana Castiglioni4Elisabetta Molteni5Gabriele Piffaretti6Patrizio Castelli7Marco Cosentino8Department of Clinical and Experimental Medicine, University of Insubria, Via Ottorino Rossi No. 9, 21100 Varese, ItalyCenter of Research in Medical Pharmacology, University of Insubria, Varese, ItalyCenter of Research in Medical Pharmacology, University of Insubria, Varese, ItalyCenter of Research in Medical Pharmacology, University of Insubria, Varese, ItalyCenter of Research in Medical Pharmacology, University of Insubria, Varese, ItalyCenter of Research in Medical Pharmacology, University of Insubria, Varese, ItalyCenter of Research in Medical Pharmacology, University of Insubria, Varese, ItalyCenter of Research in Medical Pharmacology, University of Insubria, Varese, ItalyCenter of Research in Medical Pharmacology, University of Insubria, Varese, ItalyAtherosclerosis is an inflammatory disease characterized by immunological activity, in which endothelial dysfunction represents an early event leading to subsequent inflammatory vascular damage. We investigated gene expression of the adhesion molecules (AMs) ICAM-1, VCAM-1, and β1-integrin in endothelial cells (ECs) isolated from venous blood (circulating EC, cEC) and purified from femoral plaques (pEC) obtained from 9 patients with peripheral artery disease (PAD) submitted to femoral artery thrombendarterectomy (FEA). In addition, in peripheral blood mononuclear cells (PBMCs) of the same subjects, we investigated gene expression of IFN-γ, IL-4, TGF-β, and IL-10. Patients were longitudinally evaluated 1 month before surgery, when statin treatment was established, at the time of surgery, and after 2 and 5 months. All AM mRNA levels, measured by means of real-time PCR, in cEC diminished during the study, up to 41–50% of initial levels at followup. AM mRNA expression was significantly higher in pEC than in cEC. During the study, in PBMCs, TGF-β and IL-10 mRNA levels remained unchanged while IFN-γ and IL-4 levels increased; however, the ratio IFN-γ/IL-4 showed no significant modification. In PAD patients, FEA and statin treatment induce a profound reduction of AM expression in cEC and affect cytokine mRNA expression in PBMCs.http://dx.doi.org/10.1155/2013/412761 |
| spellingShingle | Franca Marino Luigina Guasti Matteo Tozzi Laura Schembri Luana Castiglioni Elisabetta Molteni Gabriele Piffaretti Patrizio Castelli Marco Cosentino Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment International Journal of Vascular Medicine |
| title | Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment |
| title_full | Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment |
| title_fullStr | Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment |
| title_full_unstemmed | Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment |
| title_short | Gene Expression of Adhesion Molecules in Endothelial Cells from Patients with Peripheral Arterial Disease Is Reduced after Surgical Revascularization and Pharmacological Treatment |
| title_sort | gene expression of adhesion molecules in endothelial cells from patients with peripheral arterial disease is reduced after surgical revascularization and pharmacological treatment |
| url | http://dx.doi.org/10.1155/2013/412761 |
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