Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating Hemostasis
Most of the existing hydrogel dressings have inadequacies in mechanical performance, biological activities, compatibility, or versatility, which results in the development of rapid, green, and cost-effective approaches for hydrogels in biochemical and biomedical applications becoming a top-priority...
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MDPI AG
2025-01-01
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| author | Hui-Fang Lin Yue-Yue Wang Feng-Zhen Liu Zi-Wei Yang Hao Cui Si-Yu Hu Feng-He Li Pei Pan |
| author_facet | Hui-Fang Lin Yue-Yue Wang Feng-Zhen Liu Zi-Wei Yang Hao Cui Si-Yu Hu Feng-He Li Pei Pan |
| author_sort | Hui-Fang Lin |
| collection | DOAJ |
| description | Most of the existing hydrogel dressings have inadequacies in mechanical performance, biological activities, compatibility, or versatility, which results in the development of rapid, green, and cost-effective approaches for hydrogels in biochemical and biomedical applications becoming a top-priority task. Herein, inspired by the inherent bioactivity, water retention properties, and biocompatibility of natural polysaccharide hydrogels, we have prepared self-healing gels. Using <i>Bletilla striata</i> polysaccharide (BSP), carboxymethyl chitosan (CMCS), and borax via borate ester linkages, we created hemostatic and self-healing Chinese herbal medicine hydrogels in varying concentrations (2.5%, 3.0%, and 4.0%). A rotational rheometer was used to describe the hydrogels’ shape and rheological characteristics. At all concentrations, it was found that the hydrogels’ elastic modulus (G′) consistently and significantly outperformed their viscous modulus (G″), suggesting a robust internal structure. All of the hydrogels had cell viability levels as high as 100% and hemolysis rates below 1%, indicating the hydrogels’ outstanding biocompatibility. Furthermore, the hydrogels demonstrated superior hemostatic qualities in an in vivo mouse tail amputation model, as well as in in vitro coagulation tests. The results show that the hydrogel possesses excellent self-healing properties, as well as a good biocompatibility and hemostatic performance, thus paving the way for the development of a potential hemostatic green hydrogel. |
| format | Article |
| id | doaj-art-4628e05c4f264a458724d278543112f0 |
| institution | Kabale University |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-4628e05c4f264a458724d278543112f02025-01-24T13:33:54ZengMDPI AGGels2310-28612025-01-011114810.3390/gels11010048Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating HemostasisHui-Fang Lin0Yue-Yue Wang1Feng-Zhen Liu2Zi-Wei Yang3Hao Cui4Si-Yu Hu5Feng-He Li6Pei Pan7School of Pharmacy, Anhui Medical University, Hefei 230032, ChinaSchool of Pharmacy, Anhui Medical University, Hefei 230032, ChinaSchool of Pharmacy, Anhui Medical University, Hefei 230032, ChinaSchool of Pharmacy, Anhui Medical University, Hefei 230032, ChinaSchool of Pharmacy, Anhui Medical University, Hefei 230032, ChinaSchool of Pharmacy, Anhui Medical University, Hefei 230032, ChinaSchool of Pharmacy, Anhui Medical University, Hefei 230032, ChinaSchool of Pharmacy, Anhui Medical University, Hefei 230032, ChinaMost of the existing hydrogel dressings have inadequacies in mechanical performance, biological activities, compatibility, or versatility, which results in the development of rapid, green, and cost-effective approaches for hydrogels in biochemical and biomedical applications becoming a top-priority task. Herein, inspired by the inherent bioactivity, water retention properties, and biocompatibility of natural polysaccharide hydrogels, we have prepared self-healing gels. Using <i>Bletilla striata</i> polysaccharide (BSP), carboxymethyl chitosan (CMCS), and borax via borate ester linkages, we created hemostatic and self-healing Chinese herbal medicine hydrogels in varying concentrations (2.5%, 3.0%, and 4.0%). A rotational rheometer was used to describe the hydrogels’ shape and rheological characteristics. At all concentrations, it was found that the hydrogels’ elastic modulus (G′) consistently and significantly outperformed their viscous modulus (G″), suggesting a robust internal structure. All of the hydrogels had cell viability levels as high as 100% and hemolysis rates below 1%, indicating the hydrogels’ outstanding biocompatibility. Furthermore, the hydrogels demonstrated superior hemostatic qualities in an in vivo mouse tail amputation model, as well as in in vitro coagulation tests. The results show that the hydrogel possesses excellent self-healing properties, as well as a good biocompatibility and hemostatic performance, thus paving the way for the development of a potential hemostatic green hydrogel.https://www.mdpi.com/2310-2861/11/1/48<i>Bletilla striata</i> polysaccharidehydrogelsnaturalborate ester bondshemostasis |
| spellingShingle | Hui-Fang Lin Yue-Yue Wang Feng-Zhen Liu Zi-Wei Yang Hao Cui Si-Yu Hu Feng-He Li Pei Pan Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating Hemostasis Gels <i>Bletilla striata</i> polysaccharide hydrogels natural borate ester bonds hemostasis |
| title | Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating Hemostasis |
| title_full | Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating Hemostasis |
| title_fullStr | Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating Hemostasis |
| title_full_unstemmed | Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating Hemostasis |
| title_short | Natural <i>Bletilla striata</i> Polysaccharide-Based Hydrogels for Accelerating Hemostasis |
| title_sort | natural i bletilla striata i polysaccharide based hydrogels for accelerating hemostasis |
| topic | <i>Bletilla striata</i> polysaccharide hydrogels natural borate ester bonds hemostasis |
| url | https://www.mdpi.com/2310-2861/11/1/48 |
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