Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1
Smokers frequently suffer from impaired fracture healing often due to poor bone quality and stability. Cigarette smoking harms bone cells and their homeostasis by increased formation of reactive oxygen species (ROS). The aim of this study was to investigate whether Quercetin, a naturally occurring a...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2011/471426 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567994218184704 |
|---|---|
| author | Karl F. Braun Sabrina Ehnert Thomas Freude José T. Egaña Thilo L. Schenck Arne Buchholz Andreas Schmitt Sebastian Siebenlist Lilianna Schyschka Markus Neumaier Ulrich Stöckle Andreas K. Nussler |
| author_facet | Karl F. Braun Sabrina Ehnert Thomas Freude José T. Egaña Thilo L. Schenck Arne Buchholz Andreas Schmitt Sebastian Siebenlist Lilianna Schyschka Markus Neumaier Ulrich Stöckle Andreas K. Nussler |
| author_sort | Karl F. Braun |
| collection | DOAJ |
| description | Smokers frequently suffer from impaired fracture healing often due to poor bone quality and stability. Cigarette smoking harms bone cells and their homeostasis by increased formation of reactive oxygen species (ROS). The aim of this study was to investigate whether Quercetin, a naturally occurring antioxidant, can protect osteoblasts from the toxic effects of smoking. Human osteoblasts exposed to cigarette smoke medium (CSM) rapidly produced ROS and their viability decreased concentration- and time-dependently. Co-, pre- and postincubation with Quercetin dose-dependently improved their viability. Quercetin increased the expression of the anti-oxidative enzymes heme-oxygenase- (HO-) 1 and superoxide-dismutase- (SOD-) 1. Inhibiting HO-1 activity abolished the protective effect of Quercetin. Our results demonstrate that CSM damages human osteoblasts by accumulation of ROS. Quercetin can diminish this damage by scavenging the radicals and by upregulating the expression of HO-1 and SOD-1. Thus, a dietary supplementation with Quercetin could improve bone matter, stability and even fracture healing in smokers. |
| format | Article |
| id | doaj-art-4607f4c9b6694bc794ec388806eec1ad |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-4607f4c9b6694bc794ec388806eec1ad2025-02-03T00:59:56ZengWileyThe Scientific World Journal1537-744X2011-01-01112348235710.1100/2011/471426471426Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1Karl F. Braun0Sabrina Ehnert1Thomas Freude2José T. Egaña3Thilo L. Schenck4Arne Buchholz5Andreas Schmitt6Sebastian Siebenlist7Lilianna Schyschka8Markus Neumaier9Ulrich Stöckle10Andreas K. Nussler11Department of Traumatology, MRI, Techincal University of Munich, 80333 Munich, GermanyDepartment of Traumatology, University of Tübingen, Schnarrenbergstrße 95, 72076 Tübingen, GermanyDepartment of Traumatology, University of Tübingen, Schnarrenbergstrße 95, 72076 Tübingen, GermanyDepartment of Plastic Surgery and Hand Surgery, Techincal University of Munich, 80333 Munich, GermanyDepartment of Plastic Surgery and Hand Surgery, Techincal University of Munich, 80333 Munich, GermanyDepartment of Traumatology, MRI, Techincal University of Munich, 80333 Munich, GermanyDepartment of Traumatology, MRI, Techincal University of Munich, 80333 Munich, GermanyDepartment of Traumatology, MRI, Techincal University of Munich, 80333 Munich, GermanyDepartment of Traumatology, MRI, Techincal University of Munich, 80333 Munich, GermanyDepartment of Traumatology, MRI, Techincal University of Munich, 80333 Munich, GermanyDepartment of Traumatology, University of Tübingen, Schnarrenbergstrße 95, 72076 Tübingen, GermanyDepartment of Traumatology, MRI, Techincal University of Munich, 80333 Munich, GermanySmokers frequently suffer from impaired fracture healing often due to poor bone quality and stability. Cigarette smoking harms bone cells and their homeostasis by increased formation of reactive oxygen species (ROS). The aim of this study was to investigate whether Quercetin, a naturally occurring antioxidant, can protect osteoblasts from the toxic effects of smoking. Human osteoblasts exposed to cigarette smoke medium (CSM) rapidly produced ROS and their viability decreased concentration- and time-dependently. Co-, pre- and postincubation with Quercetin dose-dependently improved their viability. Quercetin increased the expression of the anti-oxidative enzymes heme-oxygenase- (HO-) 1 and superoxide-dismutase- (SOD-) 1. Inhibiting HO-1 activity abolished the protective effect of Quercetin. Our results demonstrate that CSM damages human osteoblasts by accumulation of ROS. Quercetin can diminish this damage by scavenging the radicals and by upregulating the expression of HO-1 and SOD-1. Thus, a dietary supplementation with Quercetin could improve bone matter, stability and even fracture healing in smokers.http://dx.doi.org/10.1100/2011/471426 |
| spellingShingle | Karl F. Braun Sabrina Ehnert Thomas Freude José T. Egaña Thilo L. Schenck Arne Buchholz Andreas Schmitt Sebastian Siebenlist Lilianna Schyschka Markus Neumaier Ulrich Stöckle Andreas K. Nussler Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1 The Scientific World Journal |
| title | Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1 |
| title_full | Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1 |
| title_fullStr | Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1 |
| title_full_unstemmed | Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1 |
| title_short | Quercetin Protects Primary Human Osteoblasts Exposed to Cigarette Smoke through Activation of the Antioxidative Enzymes HO-1 and SOD-1 |
| title_sort | quercetin protects primary human osteoblasts exposed to cigarette smoke through activation of the antioxidative enzymes ho 1 and sod 1 |
| url | http://dx.doi.org/10.1100/2011/471426 |
| work_keys_str_mv | AT karlfbraun quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT sabrinaehnert quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT thomasfreude quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT josetegana quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT thilolschenck quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT arnebuchholz quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT andreasschmitt quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT sebastiansiebenlist quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT liliannaschyschka quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT markusneumaier quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT ulrichstockle quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 AT andreasknussler quercetinprotectsprimaryhumanosteoblastsexposedtocigarettesmokethroughactivationoftheantioxidativeenzymesho1andsod1 |