Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureus
Abstract Background The excessive use of antibiotics is a major contributor to the global issue of antimicrobial resistance (AMR), a significant threat to human and animal health. Hence, assessing new strategies for managing Multi-Drug Resistant (MDR) microorganisms is vital. In this study, the use...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12866-025-03747-5 |
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| author | Muhammed Abdelhameed Ismael Alcici Salma Waheed Abdelhaleem Karima Mogahed Fahim Neveen Mohamed Saleh Heba Saeed Farag |
| author_facet | Muhammed Abdelhameed Ismael Alcici Salma Waheed Abdelhaleem Karima Mogahed Fahim Neveen Mohamed Saleh Heba Saeed Farag |
| author_sort | Muhammed Abdelhameed Ismael Alcici |
| collection | DOAJ |
| description | Abstract Background The excessive use of antibiotics is a major contributor to the global issue of antimicrobial resistance (AMR), a significant threat to human and animal health. Hence, assessing new strategies for managing Multi-Drug Resistant (MDR) microorganisms is vital. In this study, the use of mechanically isolated mature adipose cells (MIMACs) and their lysate (Adipolysate) as a new sustainable antimicrobial agent was assessed against Methicillin-resistant Staphylococcus aureus (MRSA). Conclusions The minimum volume of MIMACs achieved complete bacterial inhibition (Minimum Lethal volume) was 75 µl and 100 µl for bacterial concentration of 1010 and 1012 cfu/ml, respectively. Direct bacterial membrane attachment and intracellular capture was visualized under light and electron microscopy. Adipolysate was characterized via GC–MS, the fatty acid profile demonstrated several components with known antimicrobial properties. The tested Adipolysate revealed inhibition zone of diameter 25.33 ± 0.88 mm against the tested S. aureus strain, compared with the inhibition zone of Vancomycin (24.0 ± 0.00 mm) and Erythromycin (30.0 ± 0.00). The study revealed the potential effects of MIMACs and Adipolysate as sustainable, natural, and robust antimicrobial agents. However, these preliminary results will be further investigated to understand the mechanism of action and explore possible applications in various fields. |
| format | Article |
| id | doaj-art-45f4a2398ad54563abfc605c4005ca17 |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-45f4a2398ad54563abfc605c4005ca172025-02-02T12:11:10ZengBMCBMC Microbiology1471-21802025-01-0125111110.1186/s12866-025-03747-5Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureusMuhammed Abdelhameed Ismael Alcici0Salma Waheed Abdelhaleem1Karima Mogahed Fahim2Neveen Mohamed Saleh3Heba Saeed Farag4Department of Virology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Food Hygiene and Control, Faculty of Veterinary Medicine, Cairo UniversityEgyptian Drug Authority (former National Organization for Drug Control and Research), University of Nebraska Medical Center (UNMC)Department of Internal Medicine and Infectious Diseases (Infectious Diseases), Faulty of Veterinary Medicine, Cairo UniversityAbstract Background The excessive use of antibiotics is a major contributor to the global issue of antimicrobial resistance (AMR), a significant threat to human and animal health. Hence, assessing new strategies for managing Multi-Drug Resistant (MDR) microorganisms is vital. In this study, the use of mechanically isolated mature adipose cells (MIMACs) and their lysate (Adipolysate) as a new sustainable antimicrobial agent was assessed against Methicillin-resistant Staphylococcus aureus (MRSA). Conclusions The minimum volume of MIMACs achieved complete bacterial inhibition (Minimum Lethal volume) was 75 µl and 100 µl for bacterial concentration of 1010 and 1012 cfu/ml, respectively. Direct bacterial membrane attachment and intracellular capture was visualized under light and electron microscopy. Adipolysate was characterized via GC–MS, the fatty acid profile demonstrated several components with known antimicrobial properties. The tested Adipolysate revealed inhibition zone of diameter 25.33 ± 0.88 mm against the tested S. aureus strain, compared with the inhibition zone of Vancomycin (24.0 ± 0.00 mm) and Erythromycin (30.0 ± 0.00). The study revealed the potential effects of MIMACs and Adipolysate as sustainable, natural, and robust antimicrobial agents. However, these preliminary results will be further investigated to understand the mechanism of action and explore possible applications in various fields.https://doi.org/10.1186/s12866-025-03747-5MIMACsAdipocytesAdipolysateAntimicrobial ResistanceMRSASustainability |
| spellingShingle | Muhammed Abdelhameed Ismael Alcici Salma Waheed Abdelhaleem Karima Mogahed Fahim Neveen Mohamed Saleh Heba Saeed Farag Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureus BMC Microbiology MIMACs Adipocytes Adipolysate Antimicrobial Resistance MRSA Sustainability |
| title | Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureus |
| title_full | Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureus |
| title_fullStr | Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureus |
| title_short | Sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against Methicillin-resistant Staphylococcus aureus |
| title_sort | sustainable utilization of bovine adipose tissue derivatives as robust antimicrobial agents against methicillin resistant staphylococcus aureus |
| topic | MIMACs Adipocytes Adipolysate Antimicrobial Resistance MRSA Sustainability |
| url | https://doi.org/10.1186/s12866-025-03747-5 |
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