Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation
Purpose. To evaluate the regulating effect of Notch-Hes1 signaling on IL-17A+γδ+T cell expression and IL-17A secretion in mouse psoriasis-like skin inflammation. Materials and Methods. Experimental mice were randomly divided into control group, model group (5% imiquimod- (IMQ-) treated mice), and in...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/8297134 |
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| author | Yanqin Wang Xinxin Li Xiaoyun Xing Haibo Xue Ruiqun Qi Hong Ji Lei Ma |
| author_facet | Yanqin Wang Xinxin Li Xiaoyun Xing Haibo Xue Ruiqun Qi Hong Ji Lei Ma |
| author_sort | Yanqin Wang |
| collection | DOAJ |
| description | Purpose. To evaluate the regulating effect of Notch-Hes1 signaling on IL-17A+γδ+T cell expression and IL-17A secretion in mouse psoriasis-like skin inflammation. Materials and Methods. Experimental mice were randomly divided into control group, model group (5% imiquimod- (IMQ-) treated mice), and intervention group (IMQ and γ-secretase inhibitor DAPT cotreated mice). The severity of psoriasis-like skin inflammation was evaluated by target lesion score based on the clinical psoriasis area and severity index (PASI). Flow cytometry detected IL-17A+γδ+T cell percentage. Quantitative real-time RT-PCR detected Hes1 mRNA expression. Enzyme-linked immunosorbent assay and western blot measured IL-17A serum concentration and protein expression. Additionally, splenic single cells from model mice were treated by DAPT to further evaluate the inhibitory effect of blocking Notch-Hes1 signaling on IL-17A+γδ+T cell differentiation and IL-17A secretion. Results. The spleen index, IL-17A+γδ+T cell percentage, Hes1 mRNA expression, IL-17A serum concentration, and protein expression were all significantly higher in model mice than control mice, while dramatically reduced in intervention mice by DAPT treatment, which also obviously alleviated the target lesion score, epidermal hyperplasia, and dermal inflammatory cell infiltration of intervention mice. In vitro study demonstrated that DAPT treatment could result in dose-dependent decrease of IL-17A+γδ+T cell percentage and IL-17A secretion in splenic single cells of model mice. |
| format | Article |
| id | doaj-art-45eadffe21f44e3d84410f11ca03e869 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
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| series | Mediators of Inflammation |
| spelling | doaj-art-45eadffe21f44e3d84410f11ca03e8692025-02-03T05:44:15ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/82971348297134Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin InflammationYanqin Wang0Xinxin Li1Xiaoyun Xing2Haibo Xue3Ruiqun Qi4Hong Ji5Lei Ma6Department of Dermatology, Binzhou Medical University Hospital, 661 Second Huanghe Road, Binzhou 256603, ChinaDepartment of Dermatology, Binzhou Medical University Hospital, 661 Second Huanghe Road, Binzhou 256603, ChinaDepartment of Dermatology, Binzhou Medical University Hospital, 661 Second Huanghe Road, Binzhou 256603, ChinaDepartment of Endocrinology and Metabolism, Binzhou Medical University Hospital, 661 Second Huanghe Road, Binzhou 256603, ChinaDepartment of Dermatology, The First Affiliated Hospital of China Medical University, 155 North Nanjing Road, Shenyang 110001, ChinaDepartment of Pathology, Binzhou Medical University Hospital, 661 Second Huanghe Road, Binzhou 256603, ChinaDepartment of Dermatology, Binzhou Medical University Hospital, 661 Second Huanghe Road, Binzhou 256603, ChinaPurpose. To evaluate the regulating effect of Notch-Hes1 signaling on IL-17A+γδ+T cell expression and IL-17A secretion in mouse psoriasis-like skin inflammation. Materials and Methods. Experimental mice were randomly divided into control group, model group (5% imiquimod- (IMQ-) treated mice), and intervention group (IMQ and γ-secretase inhibitor DAPT cotreated mice). The severity of psoriasis-like skin inflammation was evaluated by target lesion score based on the clinical psoriasis area and severity index (PASI). Flow cytometry detected IL-17A+γδ+T cell percentage. Quantitative real-time RT-PCR detected Hes1 mRNA expression. Enzyme-linked immunosorbent assay and western blot measured IL-17A serum concentration and protein expression. Additionally, splenic single cells from model mice were treated by DAPT to further evaluate the inhibitory effect of blocking Notch-Hes1 signaling on IL-17A+γδ+T cell differentiation and IL-17A secretion. Results. The spleen index, IL-17A+γδ+T cell percentage, Hes1 mRNA expression, IL-17A serum concentration, and protein expression were all significantly higher in model mice than control mice, while dramatically reduced in intervention mice by DAPT treatment, which also obviously alleviated the target lesion score, epidermal hyperplasia, and dermal inflammatory cell infiltration of intervention mice. In vitro study demonstrated that DAPT treatment could result in dose-dependent decrease of IL-17A+γδ+T cell percentage and IL-17A secretion in splenic single cells of model mice.http://dx.doi.org/10.1155/2020/8297134 |
| spellingShingle | Yanqin Wang Xinxin Li Xiaoyun Xing Haibo Xue Ruiqun Qi Hong Ji Lei Ma Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation Mediators of Inflammation |
| title | Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation |
| title_full | Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation |
| title_fullStr | Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation |
| title_full_unstemmed | Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation |
| title_short | Notch-Hes1 Signaling Regulates IL-17A+γδ+T Cell Expression and IL-17A Secretion of Mouse Psoriasis-Like Skin Inflammation |
| title_sort | notch hes1 signaling regulates il 17a γδ t cell expression and il 17a secretion of mouse psoriasis like skin inflammation |
| url | http://dx.doi.org/10.1155/2020/8297134 |
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