Histomorphometric analysis of external apical root resorption using L-loops and different intrusive forces
Purpose This study aimed to conduct a histomorphometric analysis on the impact of varying intrusive forces on external apical root resorption (EARR) in rat molars, using an L-loop was employed to apply intrusion forces, artificially inducing EARR in the maxillary left first molar (M1) of rats.Materi...
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Taylor & Francis Group
2024-10-01
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| Series: | Clinical and Investigative Orthodontics |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/27705781.2024.2437896 |
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| author | Yosuke Miyaji Hidetoshi Morikuni Toru Tsujibayashi Yoshitomo Honda AKI Nishiura |
| author_facet | Yosuke Miyaji Hidetoshi Morikuni Toru Tsujibayashi Yoshitomo Honda AKI Nishiura |
| author_sort | Yosuke Miyaji |
| collection | DOAJ |
| description | Purpose This study aimed to conduct a histomorphometric analysis on the impact of varying intrusive forces on external apical root resorption (EARR) in rat molars, using an L-loop was employed to apply intrusion forces, artificially inducing EARR in the maxillary left first molar (M1) of rats.Materials and methods The L-loop was bent on a plaster rat model using stainless steel round wires with diameters of 0.014 and 0.016 inches. Using a universal test force measuring device, L-loops were calibrated to 4 N/mm and 6 N/mm for the 0.014-inch and 0.016-inch diameter stainless steel round wires, respectively. Fifteen-week-old Sprague-Dawley (SD) male rats were used and divided into the control (no loop) and experimental (0.014 and 0.016) groups (n = 5 for each group). The L-loop was attached to the left maxillary molar, applying an intrusive force to M1. After 15 days, the rats were euthanized. Maxillary bones were analysed using micro-computed tomography (μCT), evaluating M1 intrusive distance of vertical tooth movement and counting the number of tartrate-resistant acid phosphatase (TRAP)-positive cells on the surface of the mesial root apex and alveolar bone.Results The L-loop groups exhibited significantly increased M1 intrusive distance and TRAP-positive cell counts compared to controls. Specifically, the 0.016 group demonstrated greater M1 intrusive distance and more TRAP-positive cells than the 0.014 group. The M1 intrusive distance and TRAP-positive cells increased when intrusive forces were applied using L-loops of different diameters.Conclusion Greater intrusive forces enhance TRAP-positive cell activity, influencing EARR and vertical tooth movement. |
| format | Article |
| id | doaj-art-45d8071f300549a6b42e336841a707d8 |
| institution | Kabale University |
| issn | 2770-5781 2770-579X |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Clinical and Investigative Orthodontics |
| spelling | doaj-art-45d8071f300549a6b42e336841a707d82025-01-28T04:01:17ZengTaylor & Francis GroupClinical and Investigative Orthodontics2770-57812770-579X2024-10-0183417318110.1080/27705781.2024.2437896Histomorphometric analysis of external apical root resorption using L-loops and different intrusive forcesYosuke Miyaji0Hidetoshi Morikuni1Toru Tsujibayashi2Yoshitomo Honda3AKI Nishiura4Graduate School of Dentistry (Orthodontics), Osaka Dental University, Hirakata, Osaka, JapanDepartment of Orthodontics, Osaka Dental University, Hirakata, Osaka, JapanDepartment of Physics, Osaka Dental University, Hirakata, Osaka, JapanDepartment of Oral Anatomy, Osaka Dental University, Hirakata, Osaka, JapanDepartment of Orthodontics, Osaka Dental University, Hirakata, Osaka, JapanPurpose This study aimed to conduct a histomorphometric analysis on the impact of varying intrusive forces on external apical root resorption (EARR) in rat molars, using an L-loop was employed to apply intrusion forces, artificially inducing EARR in the maxillary left first molar (M1) of rats.Materials and methods The L-loop was bent on a plaster rat model using stainless steel round wires with diameters of 0.014 and 0.016 inches. Using a universal test force measuring device, L-loops were calibrated to 4 N/mm and 6 N/mm for the 0.014-inch and 0.016-inch diameter stainless steel round wires, respectively. Fifteen-week-old Sprague-Dawley (SD) male rats were used and divided into the control (no loop) and experimental (0.014 and 0.016) groups (n = 5 for each group). The L-loop was attached to the left maxillary molar, applying an intrusive force to M1. After 15 days, the rats were euthanized. Maxillary bones were analysed using micro-computed tomography (μCT), evaluating M1 intrusive distance of vertical tooth movement and counting the number of tartrate-resistant acid phosphatase (TRAP)-positive cells on the surface of the mesial root apex and alveolar bone.Results The L-loop groups exhibited significantly increased M1 intrusive distance and TRAP-positive cell counts compared to controls. Specifically, the 0.016 group demonstrated greater M1 intrusive distance and more TRAP-positive cells than the 0.014 group. The M1 intrusive distance and TRAP-positive cells increased when intrusive forces were applied using L-loops of different diameters.Conclusion Greater intrusive forces enhance TRAP-positive cell activity, influencing EARR and vertical tooth movement.https://www.tandfonline.com/doi/10.1080/27705781.2024.2437896L-loopexternal apical root resorptionintrusive forceintrusive distanceTrap-positive cells |
| spellingShingle | Yosuke Miyaji Hidetoshi Morikuni Toru Tsujibayashi Yoshitomo Honda AKI Nishiura Histomorphometric analysis of external apical root resorption using L-loops and different intrusive forces Clinical and Investigative Orthodontics L-loop external apical root resorption intrusive force intrusive distance Trap-positive cells |
| title | Histomorphometric analysis of external apical root resorption using L-loops and different intrusive forces |
| title_full | Histomorphometric analysis of external apical root resorption using L-loops and different intrusive forces |
| title_fullStr | Histomorphometric analysis of external apical root resorption using L-loops and different intrusive forces |
| title_full_unstemmed | Histomorphometric analysis of external apical root resorption using L-loops and different intrusive forces |
| title_short | Histomorphometric analysis of external apical root resorption using L-loops and different intrusive forces |
| title_sort | histomorphometric analysis of external apical root resorption using l loops and different intrusive forces |
| topic | L-loop external apical root resorption intrusive force intrusive distance Trap-positive cells |
| url | https://www.tandfonline.com/doi/10.1080/27705781.2024.2437896 |
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