Nanobiotechnological Nanocapsules Containing Polyhemoglobin-Tyrosinase: Effects on Murine B16F10 Melanoma Cell Proliferation and Attachment
We have reported previously that daily intravenous infusions of a soluble nanobiotechnological complex, polyhemoglobin-tyrosinase [polyHb-Tyr], can suppress the growth of murine B16F10 melanoma in a mouse model. In order to avoid the need for daily intravenous injections, we have now extended this f...
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Wiley
2012-01-01
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| Series: | Journal of Skin Cancer |
| Online Access: | http://dx.doi.org/10.1155/2012/673291 |
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| author | Yun Wang Thomas M. S. Chang |
| author_facet | Yun Wang Thomas M. S. Chang |
| author_sort | Yun Wang |
| collection | DOAJ |
| description | We have reported previously that daily intravenous infusions of a soluble nanobiotechnological complex, polyhemoglobin-tyrosinase [polyHb-Tyr], can suppress the growth of murine B16F10 melanoma in a mouse model. In order to avoid the need for daily intravenous injections, we have now extended this further as follows. We have prepared two types of biodegradable nanocapsules containing [polyHb-Tyr]. One type is to increase the circulation time and decrease the frequency of injection and is based on polyethyleneglycol-polylactic acid (PEG-PLA) nanocapsules containing [polyHb-Tyr]. The other type is to allow for intratumoural or local injection and is based on polylactic acid (PLA) nanocapsules containing [polyHb-Tyr]. Cell culture studies show that it can inhibit the proliferation of murine B16F10 melanoma cells in the “proliferation model”. It can also inhibit the attachment of murine B16F10 melanoma cells in the “attachment model.” This could be due to the action of tyrosinase on the depletion of tyrosine or the toxic effect of tyrosine metabolites. The other component, polyhemoglobin (polyHb), plays a smaller role in nanocapsules containing [polyHb-Tyr], and this is most likely by its depletion of nitric oxide needed for melanoma cell growth. |
| format | Article |
| id | doaj-art-45c824710917415ca6447ab8da9cd2bd |
| institution | Kabale University |
| issn | 2090-2905 2090-2913 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Skin Cancer |
| spelling | doaj-art-45c824710917415ca6447ab8da9cd2bd2025-02-03T01:06:39ZengWileyJournal of Skin Cancer2090-29052090-29132012-01-01201210.1155/2012/673291673291Nanobiotechnological Nanocapsules Containing Polyhemoglobin-Tyrosinase: Effects on Murine B16F10 Melanoma Cell Proliferation and AttachmentYun Wang0Thomas M. S. Chang1Artificial Cells and Organs Research Centre, Departments of Physiology, Medicine and Biomedical Engineering, Faulty of Medicine, McGill University, Room 1004, 3655 Drummond Street, Montreal, QC, H3G 1Y6, CanadaArtificial Cells and Organs Research Centre, Departments of Physiology, Medicine and Biomedical Engineering, Faulty of Medicine, McGill University, Room 1004, 3655 Drummond Street, Montreal, QC, H3G 1Y6, CanadaWe have reported previously that daily intravenous infusions of a soluble nanobiotechnological complex, polyhemoglobin-tyrosinase [polyHb-Tyr], can suppress the growth of murine B16F10 melanoma in a mouse model. In order to avoid the need for daily intravenous injections, we have now extended this further as follows. We have prepared two types of biodegradable nanocapsules containing [polyHb-Tyr]. One type is to increase the circulation time and decrease the frequency of injection and is based on polyethyleneglycol-polylactic acid (PEG-PLA) nanocapsules containing [polyHb-Tyr]. The other type is to allow for intratumoural or local injection and is based on polylactic acid (PLA) nanocapsules containing [polyHb-Tyr]. Cell culture studies show that it can inhibit the proliferation of murine B16F10 melanoma cells in the “proliferation model”. It can also inhibit the attachment of murine B16F10 melanoma cells in the “attachment model.” This could be due to the action of tyrosinase on the depletion of tyrosine or the toxic effect of tyrosine metabolites. The other component, polyhemoglobin (polyHb), plays a smaller role in nanocapsules containing [polyHb-Tyr], and this is most likely by its depletion of nitric oxide needed for melanoma cell growth.http://dx.doi.org/10.1155/2012/673291 |
| spellingShingle | Yun Wang Thomas M. S. Chang Nanobiotechnological Nanocapsules Containing Polyhemoglobin-Tyrosinase: Effects on Murine B16F10 Melanoma Cell Proliferation and Attachment Journal of Skin Cancer |
| title | Nanobiotechnological Nanocapsules Containing Polyhemoglobin-Tyrosinase: Effects on Murine B16F10 Melanoma Cell Proliferation and Attachment |
| title_full | Nanobiotechnological Nanocapsules Containing Polyhemoglobin-Tyrosinase: Effects on Murine B16F10 Melanoma Cell Proliferation and Attachment |
| title_fullStr | Nanobiotechnological Nanocapsules Containing Polyhemoglobin-Tyrosinase: Effects on Murine B16F10 Melanoma Cell Proliferation and Attachment |
| title_full_unstemmed | Nanobiotechnological Nanocapsules Containing Polyhemoglobin-Tyrosinase: Effects on Murine B16F10 Melanoma Cell Proliferation and Attachment |
| title_short | Nanobiotechnological Nanocapsules Containing Polyhemoglobin-Tyrosinase: Effects on Murine B16F10 Melanoma Cell Proliferation and Attachment |
| title_sort | nanobiotechnological nanocapsules containing polyhemoglobin tyrosinase effects on murine b16f10 melanoma cell proliferation and attachment |
| url | http://dx.doi.org/10.1155/2012/673291 |
| work_keys_str_mv | AT yunwang nanobiotechnologicalnanocapsulescontainingpolyhemoglobintyrosinaseeffectsonmurineb16f10melanomacellproliferationandattachment AT thomasmschang nanobiotechnologicalnanocapsulescontainingpolyhemoglobintyrosinaseeffectsonmurineb16f10melanomacellproliferationandattachment |