Whole Genome Identification and Integrated Analysis of Long Non-Coding RNAs Responding ABA-Mediated Drought Stress in <i>Panax ginseng</i> C.A. Meyer
<i>Panax ginseng</i> C.A. Meyer is a perennial herb that is used worldwide for a number of medical purposes. Long non-coding RNAs (lncRNAs) play a crucial role in diverse biological processes but still remain poorly understood in ginseng, which has limited the application of molecular br...
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2024-12-01
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| author | Peng Chen Cheng Chang Lingyao Kong |
| author_facet | Peng Chen Cheng Chang Lingyao Kong |
| author_sort | Peng Chen |
| collection | DOAJ |
| description | <i>Panax ginseng</i> C.A. Meyer is a perennial herb that is used worldwide for a number of medical purposes. Long non-coding RNAs (lncRNAs) play a crucial role in diverse biological processes but still remain poorly understood in ginseng, which has limited the application of molecular breeding in this plant. In this study, we identified 17,478 lncRNAs and 3106 novel mRNAs from ginseng by high-throughput illumine sequencing. 50 and 257 differentially expressed genes (DEGs) and DE lncRNAs (DELs) were detected under drought + ABA vs. drought conditions, respectively. The DEGs and DELs target genes main enrichment is focused on the “biosynthesis of secondary metabolites”, “starch and sucrose metabolism”, and “carbon metabolism” pathways under drought + ABA vs. drought conditions according to KEGG pathway enrichment analysis, suggesting that these secondary metabolites biosynthesis pathways might be crucial for ABA-mediated drought stress response in ginseng. Together, we identified drought stress response lncRNAs in ginseng for the first time and found that the target genes of these lncRNAs mainly regulate the biosynthesis of secondary metabolites pathway to response to drought stress. These findings also open up a new visual for molecular breeding in ginseng. |
| format | Article |
| id | doaj-art-45b87afa2b394c4fb916a134a1253f6d |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-45b87afa2b394c4fb916a134a1253f6d2025-01-24T13:27:22ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-01471510.3390/cimb47010005Whole Genome Identification and Integrated Analysis of Long Non-Coding RNAs Responding ABA-Mediated Drought Stress in <i>Panax ginseng</i> C.A. MeyerPeng Chen0Cheng Chang1Lingyao Kong2College of Life Sciences, Qingdao University, Qingdao 266071, ChinaCollege of Life Sciences, Qingdao University, Qingdao 266071, ChinaCollege of Life Sciences, Qingdao University, Qingdao 266071, China<i>Panax ginseng</i> C.A. Meyer is a perennial herb that is used worldwide for a number of medical purposes. Long non-coding RNAs (lncRNAs) play a crucial role in diverse biological processes but still remain poorly understood in ginseng, which has limited the application of molecular breeding in this plant. In this study, we identified 17,478 lncRNAs and 3106 novel mRNAs from ginseng by high-throughput illumine sequencing. 50 and 257 differentially expressed genes (DEGs) and DE lncRNAs (DELs) were detected under drought + ABA vs. drought conditions, respectively. The DEGs and DELs target genes main enrichment is focused on the “biosynthesis of secondary metabolites”, “starch and sucrose metabolism”, and “carbon metabolism” pathways under drought + ABA vs. drought conditions according to KEGG pathway enrichment analysis, suggesting that these secondary metabolites biosynthesis pathways might be crucial for ABA-mediated drought stress response in ginseng. Together, we identified drought stress response lncRNAs in ginseng for the first time and found that the target genes of these lncRNAs mainly regulate the biosynthesis of secondary metabolites pathway to response to drought stress. These findings also open up a new visual for molecular breeding in ginseng.https://www.mdpi.com/1467-3045/47/1/5long non-coding RNAhigh-throughput sequencing<i>Panax ginseng</i>ABAdrought stress |
| spellingShingle | Peng Chen Cheng Chang Lingyao Kong Whole Genome Identification and Integrated Analysis of Long Non-Coding RNAs Responding ABA-Mediated Drought Stress in <i>Panax ginseng</i> C.A. Meyer Current Issues in Molecular Biology long non-coding RNA high-throughput sequencing <i>Panax ginseng</i> ABA drought stress |
| title | Whole Genome Identification and Integrated Analysis of Long Non-Coding RNAs Responding ABA-Mediated Drought Stress in <i>Panax ginseng</i> C.A. Meyer |
| title_full | Whole Genome Identification and Integrated Analysis of Long Non-Coding RNAs Responding ABA-Mediated Drought Stress in <i>Panax ginseng</i> C.A. Meyer |
| title_fullStr | Whole Genome Identification and Integrated Analysis of Long Non-Coding RNAs Responding ABA-Mediated Drought Stress in <i>Panax ginseng</i> C.A. Meyer |
| title_full_unstemmed | Whole Genome Identification and Integrated Analysis of Long Non-Coding RNAs Responding ABA-Mediated Drought Stress in <i>Panax ginseng</i> C.A. Meyer |
| title_short | Whole Genome Identification and Integrated Analysis of Long Non-Coding RNAs Responding ABA-Mediated Drought Stress in <i>Panax ginseng</i> C.A. Meyer |
| title_sort | whole genome identification and integrated analysis of long non coding rnas responding aba mediated drought stress in i panax ginseng i c a meyer |
| topic | long non-coding RNA high-throughput sequencing <i>Panax ginseng</i> ABA drought stress |
| url | https://www.mdpi.com/1467-3045/47/1/5 |
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