Optimization and validation of a real-time PCR method for the simultaneous detection of Lactococcus garvieae and Streptococcus agalactiae in fish

Optimization and validation of a real-time PCR method for the simultaneous detection of Lactococcus garvieae and Streptococcus agalactiae in fish

Background: Lactococcus garvieae and Streptococcus agalactiae infections contribute to heavy losses in aquaculture farms worldwide. Currently, available pathogen diagnostic tools use biochemical and microbiological methods beleaguered by very low accuracy, reproducibility and specificity. Aim: To o...

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Bibliographic Details
Main Authors: Taona Zinyakasa, Farisai Chidzwondo, Tatenda Makawa, Sitokozile Sibanda, Exnevia Gomo, Tivapasi Musa, Elizabeth Gori
Format: Article
Language:English
Published: Plovdiv University Press 2025-01-01
Series:Journal of BioScience and Biotechnology
Subjects:
diagnostic tool
qpcr
zoonotic
aquaculture
specificity
sensitivity
Online Access:https://editorial.uni-plovdiv.bg/index.php/JBB/article/view/529/393
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