Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS
Microcystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatogr...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2011.17 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832545298568708096 |
|---|---|
| author | Gaodao Liang Ping Xie Jun Chen Ting Yu |
| author_facet | Gaodao Liang Ping Xie Jun Chen Ting Yu |
| author_sort | Gaodao Liang |
| collection | DOAJ |
| description | Microcystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatography combined with mass spectrometry (LC-MS). There was a remarkable difference in such relationships between MC-RR and -LR. The log DOW of MC-LR decreased from 1.63 at pH 1.0 to -1.26 at pH 6.5, and stabilized between -1.04 and -1.56 at a pH of 6.5~12.0; log DOW of MC-RR varied between -1.24 and -0.67 at a pH of 1.00~4.00, and stabilized between -1.20 and -1.54 at a pH of 4.00~12.00. The difference of hydrophobicity in acidic condition between MC-RR and -LR is important, not only for the analytical method of both toxins, but perhaps also for understanding the difference of toxicity to animals between the two toxins. |
| format | Article |
| id | doaj-art-45b1ae62b4e846f3b1fa716041dfb6ad |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-45b1ae62b4e846f3b1fa716041dfb6ad2025-02-03T07:26:08ZengWileyThe Scientific World Journal1537-744X2011-01-0111202610.1100/tsw.2011.17Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MSGaodao Liang0Ping Xie1Jun Chen2Ting Yu3Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaDonghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaDonghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaDonghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaMicrocystins (MCs) are well known worldwide as hepatotoxins produced by cyanobacteria, but little is known about the physicochemical properties of these compounds. The dependence of the n-octanol/water distribution ratio (DOW) of MC-RR and -LR to pH was measured by high-performance liquid chromatography combined with mass spectrometry (LC-MS). There was a remarkable difference in such relationships between MC-RR and -LR. The log DOW of MC-LR decreased from 1.63 at pH 1.0 to -1.26 at pH 6.5, and stabilized between -1.04 and -1.56 at a pH of 6.5~12.0; log DOW of MC-RR varied between -1.24 and -0.67 at a pH of 1.00~4.00, and stabilized between -1.20 and -1.54 at a pH of 4.00~12.00. The difference of hydrophobicity in acidic condition between MC-RR and -LR is important, not only for the analytical method of both toxins, but perhaps also for understanding the difference of toxicity to animals between the two toxins.http://dx.doi.org/10.1100/tsw.2011.17 |
| spellingShingle | Gaodao Liang Ping Xie Jun Chen Ting Yu Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS The Scientific World Journal |
| title | Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS |
| title_full | Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS |
| title_fullStr | Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS |
| title_full_unstemmed | Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS |
| title_short | Comparative Studies on the pH Dependence of DOW of Microcystin-RR and -LR Using LC-MS |
| title_sort | comparative studies on the ph dependence of dow of microcystin rr and lr using lc ms |
| url | http://dx.doi.org/10.1100/tsw.2011.17 |
| work_keys_str_mv | AT gaodaoliang comparativestudiesonthephdependenceofdowofmicrocystinrrandlrusinglcms AT pingxie comparativestudiesonthephdependenceofdowofmicrocystinrrandlrusinglcms AT junchen comparativestudiesonthephdependenceofdowofmicrocystinrrandlrusinglcms AT tingyu comparativestudiesonthephdependenceofdowofmicrocystinrrandlrusinglcms |