A Knotless Technique for Kidney Transplantation in the Mouse
Mouse models of kidney transplantation are important to study molecular mechanisms of organ transplant rejection as well as to develop new therapeutic strategies aimed at improving allograft survival. However, the surgical technique necessary to result in a viable allograft has traditionally proven...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Transplantation |
| Online Access: | http://dx.doi.org/10.1155/2012/127215 |
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| author | Song Rong Alfor G. Lewis Uta Kunter Hermann Haller Faikah Gueler |
| author_facet | Song Rong Alfor G. Lewis Uta Kunter Hermann Haller Faikah Gueler |
| author_sort | Song Rong |
| collection | DOAJ |
| description | Mouse models of kidney transplantation are important to study molecular mechanisms of organ transplant rejection as well as to develop new therapeutic strategies aimed at improving allograft survival. However, the surgical technique necessary to result in a viable allograft has traditionally proven to be complex and very demanding. Here, we introduce a new, simple, and rapid knotless technique for vessel anastomosis wherein the last stitch of the anastomosis is not tied to the short end of the upper tie as in the classical approach but is left free. This is a critical difference in that it allows the size of the anastomosis to be increased or decreased after graft reperfusion in order to avoid stenosis or bleeding, respectively. We compared the outcome of this new knotless technique (n=175) with the classical approach (n=122) in terms of local thrombosis or bleeding, time for anastomosis, and survival rates. By this modification of the suture technique, local thrombosis was significantly reduced (1.1% versus 6.6%), anastomosis time was less, and highly reproducible kidney graft survival was achieved (95% versus 84% with the classical approach). We believe that this knotless technique is easy to learn and will improve the success rates in the technically demanding model of mouse kidney transplantation. |
| format | Article |
| id | doaj-art-458dd1b472ff44d8abbec1a8200d3f1a |
| institution | Kabale University |
| issn | 2090-0007 2090-0015 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Transplantation |
| spelling | doaj-art-458dd1b472ff44d8abbec1a8200d3f1a2025-02-03T01:00:51ZengWileyJournal of Transplantation2090-00072090-00152012-01-01201210.1155/2012/127215127215A Knotless Technique for Kidney Transplantation in the MouseSong Rong0Alfor G. Lewis1Uta Kunter2Hermann Haller3Faikah Gueler4Division of Nephrology, Hannover Medical School, 30625 Hannover, GermanyDivision of Developmental Biology, Cincinnati Children's Hospital Research Foundation, Cincinnati, OH 45229, USADivision of Nephrology and Immunology, RWTH University of Aachen, 52062 Aachen, GermanyDivision of Nephrology, Hannover Medical School, 30625 Hannover, GermanyDivision of Nephrology, Hannover Medical School, 30625 Hannover, GermanyMouse models of kidney transplantation are important to study molecular mechanisms of organ transplant rejection as well as to develop new therapeutic strategies aimed at improving allograft survival. However, the surgical technique necessary to result in a viable allograft has traditionally proven to be complex and very demanding. Here, we introduce a new, simple, and rapid knotless technique for vessel anastomosis wherein the last stitch of the anastomosis is not tied to the short end of the upper tie as in the classical approach but is left free. This is a critical difference in that it allows the size of the anastomosis to be increased or decreased after graft reperfusion in order to avoid stenosis or bleeding, respectively. We compared the outcome of this new knotless technique (n=175) with the classical approach (n=122) in terms of local thrombosis or bleeding, time for anastomosis, and survival rates. By this modification of the suture technique, local thrombosis was significantly reduced (1.1% versus 6.6%), anastomosis time was less, and highly reproducible kidney graft survival was achieved (95% versus 84% with the classical approach). We believe that this knotless technique is easy to learn and will improve the success rates in the technically demanding model of mouse kidney transplantation.http://dx.doi.org/10.1155/2012/127215 |
| spellingShingle | Song Rong Alfor G. Lewis Uta Kunter Hermann Haller Faikah Gueler A Knotless Technique for Kidney Transplantation in the Mouse Journal of Transplantation |
| title | A Knotless Technique for Kidney Transplantation in the Mouse |
| title_full | A Knotless Technique for Kidney Transplantation in the Mouse |
| title_fullStr | A Knotless Technique for Kidney Transplantation in the Mouse |
| title_full_unstemmed | A Knotless Technique for Kidney Transplantation in the Mouse |
| title_short | A Knotless Technique for Kidney Transplantation in the Mouse |
| title_sort | knotless technique for kidney transplantation in the mouse |
| url | http://dx.doi.org/10.1155/2012/127215 |
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