The Feature of Distribution and Clonality of TCR γ/δ Subfamilies T Cells in Patients with B-Cell Non-Hodgkin Lymphoma
Restricted T-cell receptor (TCR) Vα/Vβ repertoire expression and clonal expansion of αβ T cells especially for putative tumor-associated antigens were observed in patients with hematological malignancies. To further characterize the γδ T-cell immune status in B-cell non-Hodgkin lymphoma (B-NHL), we...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/241246 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560592624287744 |
|---|---|
| author | Liang Wang Meng Xu Chunyan Wang Lihua Zhu Junyan Hu Shaohua Chen Xiuli Wu Bo Li Yangqiu Li |
| author_facet | Liang Wang Meng Xu Chunyan Wang Lihua Zhu Junyan Hu Shaohua Chen Xiuli Wu Bo Li Yangqiu Li |
| author_sort | Liang Wang |
| collection | DOAJ |
| description | Restricted T-cell receptor (TCR) Vα/Vβ repertoire expression and clonal expansion of αβ T cells especially for putative tumor-associated antigens were observed in patients with hematological malignancies. To further characterize the γδ T-cell immune status in B-cell non-Hodgkin lymphoma (B-NHL), we investigated the distribution and clonality of TCR Vγ/Vδ repertoire in peripheral blood (PB), bone marrow (BM), and lymph node (LN) from patients with B-NHL. Four newly diagnosed B-NHL cases, including three with diffuse large B-cell lymphoma (DLBCL) and one with small lymphocytic lymphoma (SLL), were enrolled. The restrictive expression of TCR Vγ/Vδ subfamilies with different distribution patterns could be detected in PB, BM, or LN from all of four patients, and partial subfamily T cells showed clonal proliferation. At least one clonally expanded Vδ subfamily member was found in PB from each patient. However, the expression pattern and clonality of TCR Vγ/Vδ changed in different immune organs and showed individual feature in different patients. The clonally expanded Vδ5, Vδ6, and Vδ8 were detected only in PB but neither in BM nor LN while clonally expanded Vδ2 and Vδ3 could be detected in both PB and BM/LN. In conclusion, the results provide a preliminary profile of distribution and clonality of TCR γ/δ subfamilies T cells in PB, BM, and LN from B-NHL; similar clonally expanded Vδ subfamily T cells in PB and BM may be related to the same B-cell lymphoma-associated antigens, while the different reactive clonally expanded Vγ/Vδ T cells may be due to local immune response. |
| format | Article |
| id | doaj-art-4576bfa0df434505ba9e3c8299c04881 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-4576bfa0df434505ba9e3c8299c048812025-02-03T01:27:13ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/241246241246The Feature of Distribution and Clonality of TCR γ/δ Subfamilies T Cells in Patients with B-Cell Non-Hodgkin LymphomaLiang Wang0Meng Xu1Chunyan Wang2Lihua Zhu3Junyan Hu4Shaohua Chen5Xiuli Wu6Bo Li7Yangqiu Li8Department of Oncology, First Affiliated Hospital, Jinan University, Guangzhou 510632, ChinaDepartment of Oncology, First Affiliated Hospital, Jinan University, Guangzhou 510632, ChinaCentre of Oncology and Hematology, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510230, ChinaDepartment of Rheumatism and Immunology, First Affiliated Hospital, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaInstitute of Hematology, Jinan University, Guangzhou 510632, ChinaRestricted T-cell receptor (TCR) Vα/Vβ repertoire expression and clonal expansion of αβ T cells especially for putative tumor-associated antigens were observed in patients with hematological malignancies. To further characterize the γδ T-cell immune status in B-cell non-Hodgkin lymphoma (B-NHL), we investigated the distribution and clonality of TCR Vγ/Vδ repertoire in peripheral blood (PB), bone marrow (BM), and lymph node (LN) from patients with B-NHL. Four newly diagnosed B-NHL cases, including three with diffuse large B-cell lymphoma (DLBCL) and one with small lymphocytic lymphoma (SLL), were enrolled. The restrictive expression of TCR Vγ/Vδ subfamilies with different distribution patterns could be detected in PB, BM, or LN from all of four patients, and partial subfamily T cells showed clonal proliferation. At least one clonally expanded Vδ subfamily member was found in PB from each patient. However, the expression pattern and clonality of TCR Vγ/Vδ changed in different immune organs and showed individual feature in different patients. The clonally expanded Vδ5, Vδ6, and Vδ8 were detected only in PB but neither in BM nor LN while clonally expanded Vδ2 and Vδ3 could be detected in both PB and BM/LN. In conclusion, the results provide a preliminary profile of distribution and clonality of TCR γ/δ subfamilies T cells in PB, BM, and LN from B-NHL; similar clonally expanded Vδ subfamily T cells in PB and BM may be related to the same B-cell lymphoma-associated antigens, while the different reactive clonally expanded Vγ/Vδ T cells may be due to local immune response.http://dx.doi.org/10.1155/2014/241246 |
| spellingShingle | Liang Wang Meng Xu Chunyan Wang Lihua Zhu Junyan Hu Shaohua Chen Xiuli Wu Bo Li Yangqiu Li The Feature of Distribution and Clonality of TCR γ/δ Subfamilies T Cells in Patients with B-Cell Non-Hodgkin Lymphoma Journal of Immunology Research |
| title | The Feature of Distribution and Clonality of TCR γ/δ Subfamilies T Cells in Patients with B-Cell Non-Hodgkin Lymphoma |
| title_full | The Feature of Distribution and Clonality of TCR γ/δ Subfamilies T Cells in Patients with B-Cell Non-Hodgkin Lymphoma |
| title_fullStr | The Feature of Distribution and Clonality of TCR γ/δ Subfamilies T Cells in Patients with B-Cell Non-Hodgkin Lymphoma |
| title_full_unstemmed | The Feature of Distribution and Clonality of TCR γ/δ Subfamilies T Cells in Patients with B-Cell Non-Hodgkin Lymphoma |
| title_short | The Feature of Distribution and Clonality of TCR γ/δ Subfamilies T Cells in Patients with B-Cell Non-Hodgkin Lymphoma |
| title_sort | feature of distribution and clonality of tcr γ δ subfamilies t cells in patients with b cell non hodgkin lymphoma |
| url | http://dx.doi.org/10.1155/2014/241246 |
| work_keys_str_mv | AT liangwang thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT mengxu thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT chunyanwang thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT lihuazhu thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT junyanhu thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT shaohuachen thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT xiuliwu thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT boli thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT yangqiuli thefeatureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT liangwang featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT mengxu featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT chunyanwang featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT lihuazhu featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT junyanhu featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT shaohuachen featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT xiuliwu featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT boli featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma AT yangqiuli featureofdistributionandclonalityoftcrgdsubfamiliestcellsinpatientswithbcellnonhodgkinlymphoma |